2,3,7,8-tetrachlorodibenzo-p-dioxin exposure influence the expression of glutamate transporter GLT-1 in C6 glioma cells via the Ca2+/protein kinase C pathway

2016 ◽  
Vol 36 (11) ◽  
pp. 1409-1417 ◽  
Author(s):  
Jianya Zhao ◽  
Yan Zhang ◽  
Jianmei Zhao ◽  
Cheng Wang ◽  
Jiamin Mao ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Glutamate Transporter ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Kinase C ◽  
Tetrachlorodibenzo P Dioxin ◽  
Dioxin Exposure

Isoflurane Enhances the Expression and Activity of Glutamate Transporter Type 3 in C6 Glioma Cells

2003 ◽  
Vol 99 (6) ◽  
pp. 1346-1353 ◽  
Author(s):  
Yueming Huang ◽  
Zhiyi Zuo
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Glutamate Transporter ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
C6 Cells ◽  
Phosphatidylinositol 3 Kinase ◽  
Kinase C ◽  
Type 3

Background Glutamate transporters play an important role in maintaining extracellular glutamate homeostasis. Volatile anesthetics have been shown to affect glutamate transporter activity acutely (within minutes after the exposure). It is not known whether volatile anesthetics affect the expression of glutamate transporters. Methods Rat cultured C6 glioma cells that express excitatory amino acid transporter type 3 (EAAT3) were exposed to isoflurane at various concentrations (0.5-4.0%) or for different periods (1-24 h) at 37 degrees C. EAAT3 mRNA, proteins, and activity were quantified. Results Isoflurane induced a time- and concentration-dependent increase in the mRNA and protein levels of EAAT3 in C6 cells. The maximal increase was induced by 2% isoflurane, and the cells incubated with 2% isoflurane for 3 and 7 h expressed the highest levels of EAAT3 mRNA and proteins, respectively. Similarly, glutamate uptake was higher in C6 cells exposed to 2% isoflurane for 7 h than in control cells. Actinomycin D and cycloheximide, inhibitors for mRNA and protein synthesis, respectively, did not affect the isoflurane-induced increase in EAAT3 mRNA and protein expression. Phorbol 12-myristate 13-acetate, a protein kinase C activator, also enhanced EAAT3 expression. The combination of 2% isoflurane and phorbol 12-myristate 13-acetate caused a higher level of EAAT3 expression than that induced by 2% isoflurane alone. Neither staurosporine, a protein kinase C inhibitor, nor wortmannin, a phosphatidylinositol 3 kinase inhibitor, inhibited the isoflurane-induced increase in EAAT3 expression. Conclusions The results of this study suggest that isoflurane increases the expression and activity of EAAT3 by stabilizing EAAT3 mRNA and proteins via protein kinase C- and phosphatidylinositol 3 kinase-independent pathways.

scholarly journals 2adrenoreceptor agonist regulates protein kinase C-induced heat shock protein 27 phosphorylation in C6 glioma cells

2008 ◽  
Vol 106 (2) ◽  
pp. 519-528 ◽  
Author(s):  
Kumiko Tanabe ◽  
Shinji Takai ◽  
Rie Matsushima-Nishiwaki ◽  
Kanefusa Kato ◽  
Shuji Dohi ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Heat Shock ◽  
Protein Kinase ◽  
Heat Shock Protein ◽  
Glioma Cells ◽  
Heat Shock Protein 27 ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Kinase C

scholarly journals Pinus densiflora Gnarl Inhibits Migration through Suppression of Protein Kinase C in C6 Glioma Cells

2015 ◽  
Vol 32 (2) ◽  
pp. 51-58
Author(s):  
Ilguk Min ◽  
Kangpa Lee ◽  
Haeryong Chang ◽  
Jinyoung Moon
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Pinus Densiflora ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Kinase C

Protein kinase C isoform ? overexpression in C6 glioma cells and its role in cell proliferation

1995 ◽  
Vol 24 (3) ◽  
pp. 241-250 ◽  
Author(s):  
Gordon H. Baltuch ◽  
Nora P. Dooley ◽  
Klara M. Rostworowski ◽  
Jean -Guy Villemure ◽  
Voon Wee Yong
Keyword(s):  
Cell Proliferation ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Kinase C

scholarly journals Inhibitors of actin polymerization and calmodulin binding enhance protein kinase C-induced translocation of MARCKS in C6 glioma cells

1997 ◽  
Vol 1356 (2) ◽  
pp. 121-130 ◽  
Author(s):  
Donna N Douglas ◽  
Horst-Siegfried Fink ◽  
Sergio D Rosé ◽  
Neale D Ridgway ◽  
Harold W Cook ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Actin Polymerization ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Calmodulin Binding ◽  
Kinase C

Protein expression of the α, γ, δ and ε subspecies of protein kinase C changes as C6 glioma cells become contact inhibited and quiescent in the presence of serum

FEBS Letters ◽  
1995 ◽  
Vol 372 (1) ◽  
pp. 33-38 ◽  
Author(s):  
Kevin Moreton ◽  
Rebecca Turner ◽  
Nicola Blake ◽  
Alex Paton ◽  
Nigel Groome ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Protein Expression ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Kinase C

Protein kinase C activation of phospholipase D in C6 glioma cells

1993 ◽  
Vol 21 (4) ◽  
pp. 354S-354S ◽  
Author(s):  
Barbara S. Mallon ◽  
Helen J. M. Thompson ◽  
Rebecca A. Turner ◽  
Caroline M. Holden ◽  
Martin G. Rumsby
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Phospholipase D ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Kinase C ◽  
Protein Kinase C Activation
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