Effects of water soluble PM2.5 extracts exposure on human lung epithelial cells (A549): A proteomic study

2013 ◽  
Vol 34 (6) ◽  
pp. 675-687 ◽  
Author(s):  
Qingyu Huang ◽  
Jie Zhang ◽  
Siyuan Peng ◽  
Meiping Tian ◽  
Jinsheng Chen ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Lung ◽  
Water Soluble ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Proteomic Study ◽  
Human Lung Epithelial Cells

scholarly journals Chemical Characterization of Seasonal PM2.5 Samples and Their Cytotoxicity in Human Lung Epithelial Cells (A549)

2020 ◽  
Vol 17 (12) ◽  
pp. 4599 ◽  
Author(s):  
Ao Di ◽  
Yun Wu ◽  
Mindong Chen ◽  
Dongyang Nie ◽  
Xinlei Ge
Keyword(s):  
Heavy Metals ◽  
Epithelial Cells ◽  
Human Lung ◽  
Chemical Characterization ◽  
Inorganic Ions ◽  
Water Soluble ◽  
Lung Epithelial Cells ◽  
Ros Production ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

In order to study the toxicity of fine particulate matter (PM2.5) sourced from different seasons on human health, we collected PM2.5 samples quarterly from March 2016 to February 2017 in Nanjing, China. The component analysis results showed that high proportions of water-soluble organic carbon (WSOC), SO42−, Ca2+ and Mg2+ were found in the summer samples, while high proportions of NO3−, NH4+ and heavy metals were observed in the spring and winter samples. Then human lung epithelial cells (A549) were exposed to the PM2.5 samples. The toxicological results indicated that reactive oxygen species (ROS) production in the spring and winter samples was higher than that in the summer and fall samples, which was related to the contribution of some heavy metals and inorganic ions (e.g., Pb and NO3−). However, the apoptosis rates of the cells showed the opposite seasonal changes as what the ROS did, which might be caused by the higher WSOC content in the summer. In addition, regression analysis also showed the importance of the PM2.5 components in ROS production and apoptosis. Particularly, Zn had the strongest correlation with ROS production (R = 0.863) and cell apoptosis (R = 0.675); thus, the specific toxicity of Zn in PM2.5 deserves further investigation. Our results could be beneficial for assessing the health risks and controlling the toxic components of PM2.5 in Nanjing.

Legionella pneumophila induced microRNA expression in human lung epithelial cells

Pneumologie ◽  
2010 ◽  
Vol 64 (S 03) ◽  
Author(s):  
B Schmeck ◽  
B Dolniak ◽  
I Pollock ◽  
C Schulz ◽  
W Bertrams ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Legionella Pneumophila ◽  
Human Lung ◽  
Microrna Expression ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells
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