Silver nanoparticle-induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues

2012 ◽  
Vol 33 (2) ◽  
pp. 78-89 ◽  
Author(s):  
Soohee Kim ◽  
Doug-Young Ryu
Keyword(s):  
Oxidative Stress ◽  
Silver Nanoparticle ◽  
Cultured Cells ◽  
Animal Tissues

scholarly journals Dibenzoylthiamine Has Powerful Antioxidant and Anti-Inflammatory Properties in Cultured Cells and in Mouse Models of Stress and Neurodegeneration

Biomedicines ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 361
Author(s):  
Margaux Sambon ◽  
Anna Gorlova ◽  
Alice Demelenne ◽  
Judit Alhama-Riba ◽  
Bernard Coumans ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Neurodegenerative Diseases ◽  
Cell Line ◽  
Mouse Models ◽  
Cultured Cells ◽  
Therapeutic Potential ◽  
Motor Dysfunction ◽  
Oxidative Stress Marker ◽  
Bv2 Cells ◽  
Anti Inflammatory

Thiamine precursors, the most studied being benfotiamine (BFT), have protective effects in mouse models of neurodegenerative diseases. BFT decreased oxidative stress and inflammation, two major characteristics of neurodegenerative diseases, in a neuroblastoma cell line (Neuro2a) and an immortalized brain microglial cell line (BV2). Here, we tested the potential antioxidant and anti-inflammatory effects of the hitherto unexplored derivative O,S-dibenzoylthiamine (DBT) in these two cell lines. We show that DBT protects Neuro2a cells against paraquat (PQ) toxicity by counteracting oxidative stress at low concentrations and increases the synthesis of reduced glutathione and NADPH in a Nrf2-independent manner. In BV2 cells activated by lipopolysaccharides (LPS), DBT significantly decreased inflammation by suppressing translocation of NF-κB to the nucleus. Our results also demonstrate the superiority of DBT over thiamine and other thiamine precursors, including BFT, in all of the in vitro models. Finally, we show that the chronic administration of DBT arrested motor dysfunction in FUS transgenic mice, a model of amyotrophic lateral sclerosis, and it reduced depressive-like behavior in a mouse model of ultrasound-induced stress in which it normalized oxidative stress marker levels in the brain. Together, our data suggest that DBT may have therapeutic potential for brain pathology associated with oxidative stress and inflammation by novel, coenzyme-independent mechanisms.

RNA Interference-Mediated Inhibition of Wild-Type Torsin A Expression Increases Apoptosis Caused by Oxidative Stress in Cultured Cells

2010 ◽  
Vol 35 (8) ◽  
pp. 1214-1223 ◽  
Author(s):  
Xue-Ping Chen ◽  
Xiao-Hui Hu ◽  
Shu-Hui Wu ◽  
Yang-Wei Zhang ◽  
Bo Xiao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rna Interference ◽  
Cultured Cells ◽  
Wild Type

Sites and Mechanisms of Low-Level Oxidative Stress in Cultured Cells

1995 ◽  
Vol 206 (1) ◽  
pp. 421-428 ◽  
Author(s):  
D. Gelvan ◽  
V. Moreno ◽  
D.A. Clopton ◽  
Q. Chen ◽  
P. Saltman
Keyword(s):  
Oxidative Stress ◽  
Cultured Cells ◽  
Low Level

Protective effects of hydrogen against irradiation

2021 ◽  
Vol 27 ◽  
Author(s):  
Yasuhiro Terasaki ◽  
Mika Terasaki ◽  
Akira Shimizu
Keyword(s):  
Oxidative Stress ◽  
Lung Injury ◽  
Cultured Cells ◽  
Animal Experiments ◽  
Lung Epithelial Cells ◽  
Lung Damage ◽  
Protective Effects ◽  
Chronic Radiation ◽  
Radiation Induced ◽  
Reactive Oxidants

: Radiation-induced lung injury is characterized by an acute pneumonia phase followed by a fibrotic phase. At the time of irradiation, a rapid, short-lived burst of reactive oxygen species (ROS) such as hydroxyl radicals (•OH) occurs, but chronic radiation-induced lung injury may occur due to excess ROS such as H2O2 , O2•− , ONOO− , and •OH. Molecular hydrogen (H2 ) is an efficient antioxidant that quickly diffuses cell membranes, reduces ROS such as •OH and ONOO− , and suppresses damage caused by oxidative stress in various organs. In 2011, through the evaluation of electron-spin resonance and fluorescent indicator signals, we had reported that H2 can eliminate •OH and can protect against oxidative stress-related apoptotic damage induced by irradiation of cultured lung epithelial cells. We had explored for the first time the radioprotective effects of H2 treatment on acute and chronic radiation-induced lung damage in mice by inhaled H2 gas (for acute) and imbibed H2 -enriched water (for chronic). Thus, we had proposed that H2 be considered a potential radioprotective agent. Recent publications have shown that H2 directly neutralizes highly reactive oxidants and indirectly reduces oxidative stress by regulating the expression of various genes. By regulating gene expression, H2 functions as an anti-inflammatory and anti-apoptotic molecule and promotes energy metabolism. The increased evidence obtained from cultured cells or animal experiments reveal a putative place for H2 treatment and its radioprotective effect clinically. This review focuses on major scientific advances of in the treatment of H2 as a new class of radioprotective agents.

Molecular adaptation of vascular endothelial cells to oxidative stress

1993 ◽  
Vol 264 (3) ◽  
pp. C715-C722 ◽  
Author(s):  
D. Lu ◽  
N. Maulik ◽  
I. I. Moraru ◽  
D. L. Kreutzer ◽  
D. K. Das
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Cultured Cells ◽  
Vascular Endothelial Cells ◽  
Molecular Adaptation ◽  
Vascular Endothelial ◽  
Trypan Blue Exclusion ◽  
Two Dimensional Gel Electrophoresis ◽  
H2o2 Treatment ◽  
Trypan Blue Exclusion Test

Cellular organisms respond at the cellular and molecular level when confronted with sudden changes in environment, and molecular adaptation represents the ability of the cells to acclimate themselves to their new environment. In this study we examined the response of bovine vascular endothelial cells (VEC) to the oxidative stress by exposing the cultured cells to two different concentrations of H2O2, 0.04 or 0.08 mM, for 18-24 h. H2O2-exposed VEC displayed good viability (85-90% for 0.04 mM H2O2; 75-80% for 0.08 mM H2O2) and exhibited normal morphology. H2O2 treatment of the VEC was associated with the expression of a number of new proteins, as demonstrated by two-dimensional gel electrophoresis of total cell lysate. Cells exposed to 0.04 mM H2O2 expressed 25 new proteins, whereas 19 newly expressed proteins were detected when the cells were exposed to 0.08 mM H2O2. Western blot analysis of H2O2-treated VEC using specific antibodies to heat-shock proteins (HSP) identified one of these proteins as a member of the HSP 70 family. In addition, H2O2 induced an increase in antioxidative enzyme activities in the VEC, including superoxide dismutase, catalase, and glutathione peroxidase. Moreover, these changes were a truly adaptive phenomenon because challenging the VEC with brief exposure to toxic levels of H2O2 (1 mM for 30 min) showed increased viability (by Trypan blue exclusion test) and decreased injury (by lactate dehydrogenase supernatant-to-cellular ratio determination) in adapted cells (preexposed to 0.04 or 0.08 mM H2O2) compared with control cells.(ABSTRACT TRUNCATED AT 250 WORDS)

Oxidative Stress and a Key Metabolic Enzyme in Alzheimer Brains, Cultured Cells, and an Animal Model of Chronic Oxidative Deficits

1999 ◽  
Vol 893 (1 OXIDATIVE/ENE) ◽  
pp. 79-94 ◽  
Author(s):  
GARY E. GIBSON ◽  
LARRY C. H. PARK ◽  
HUI ZHANG ◽  
SANDRO SORBI ◽  
NOEL Y. CALINGASAN
Keyword(s):  
Oxidative Stress ◽  
Animal Model ◽  
Cultured Cells ◽  
Metabolic Enzyme

Silver nanoparticle induced cytotoxicity, oxidative stress, and DNA damage in CHO cells

2013 ◽  
Vol 15 (9) ◽  
Author(s):  
Kumud Kant Awasthi ◽  
Anjali Awasthi ◽  
Narender Kumar ◽  
Partha Roy ◽  
Kamlendra Awasthi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Silver Nanoparticle ◽  
Cho Cells

Oxidative stress in primary culture hepatocytes isolated from partially hepatectomized rats

2007 ◽  
Vol 85 (10) ◽  
pp. 1047-1051 ◽  
Author(s):  
Daniel Francés ◽  
M. Teresa Ronco ◽  
Elena Ochoa ◽  
M. Luján Alvarez ◽  
Ariel Quiroga ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Primary Culture ◽  
Cell Injury ◽  
Cultured Cells ◽  
Male Wistar Rats ◽  
Metabolic Activities ◽  
Stress Oxidative ◽  
Species Production

The aim of this study was to evaluate the influence of partial hepatectomy prior to cell isolation on hepatocytes in vitro. We characterized the possible changes of various stress oxidative parameters within the first 24 h after seeding. Male Wistar rats served as donors. Hepatocytes were isolated by collagenase digestion from either liver of simulated surgery (SH) or from liver 1 h after 70% hepatectomy (PH), and the changes in stress parameters were analyzed after 1, 3, 18, and 24 h in culture. At 24 h, only hepatocytes from PH maintained significantly increased reactive oxygen species production, oxidized glutathione percentage, and Cu/Zn superoxide dismutase and catalase activities. Our results show that hepatocytes suffer significant cell injury as a result of the isolation procedure, but primary cultured cells from SH metabolically recover from this stress after 18 h. After this time, primary culture hepatocytes primed by PH maintain their in vivo-like metabolic activities (increase in both oxidative stress and antioxidant status).

scholarly journals Transcriptional Homeostasis of Oxidative Stress-Related Pathways in Altered Gravity

2018 ◽  
Vol 19 (9) ◽  
pp. 2814 ◽  
Author(s):  
Svantje Tauber ◽  
Swantje Christoffel ◽  
Cora Thiel ◽  
Oliver Ullrich
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Immune System ◽  
Cellular Response ◽  
Cultured Cells ◽  
Parabolic Flight ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Altered Gravity ◽  
Human Immune System

Whereby several types of cultured cells are sensitive to gravity, the immune system belongs to the most affected systems during spaceflight. Since reactive oxygen species/reactive nitrogen species (ROS/RNS) are serving as signals of cellular homeostasis, particularly in the cells of the immune system, we investigated the immediate effect of altered gravity on the transcription of 86 genes involved in reactive oxygen species metabolism, antioxidative systems, and cellular response to oxidative stress, using parabolic flight and suborbital ballistic rocket experiments and microarray analysis. In human myelomonocytic U937 cells, we detected a rapid response of 19.8% of all of the investigated oxidative stress-related transcripts to 1.8 g of hypergravity and 1.1% to microgravity as early as after 20 s. Nearly all (97.2%) of the initially altered transcripts adapted after 75 s of hypergravity (max. 13.5 g), and 100% adapted after 5 min of microgravity. After the almost complete adaptation of initially altered transcripts, a significant second pool of differentially expressed transcripts appeared. In contrast, we detected nearly no response of oxidative stress-related transcripts in human Jurkat T cells to altered gravity. In conclusion, we assume a very well-regulated homeostasis and transcriptional stability of oxidative stress-related pathways in altered gravity in cells of the human immune system.

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