DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells

2012 ◽  
pp. n/a-n/a ◽  
Author(s):  
Gabriela Molinari ◽  
Maciej Kujawski ◽  
Anna Scuto ◽  
Sonia Soloneski ◽  
Marcelo L. Larramendy
Keyword(s):  
Dna Damage ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells

scholarly journals Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro

2004 ◽  
Vol 18 (3) ◽  
pp. 192-196 ◽  
Author(s):  
Daniel Araki Ribeiro ◽  
Clarissa Scolastici ◽  
Mariângela Esther Alencar Marques ◽  
Daisy Maria Fávero Salvadori
Keyword(s):  
Dna Damage ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Genetic Material ◽  
Chinese Hamster ◽  
Potential Health ◽  
Ovary Cells ◽  
The Mean ◽  
Genotoxicity Tests

Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/ml for 3 h, at 37°C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents.

scholarly journals Caffeine alone causes DNA damage in Chinese hamster ovary cells.

1985 ◽  
Vol 10 (4) ◽  
pp. 405-409 ◽  
Author(s):  
Ryoji Ishida ◽  
Masahiro Kozaki ◽  
Taijo Takahashi
Keyword(s):  
Dna Damage ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells

scholarly journals Centrosomes Split in the Presence of Impaired DNA Integrity during Mitosis

2003 ◽  
Vol 14 (5) ◽  
pp. 1993-2004 ◽  
Author(s):  
Henderika M.J. Hut ◽  
Willy Lemstra ◽  
Engbert H. Blaauw ◽  
Gert W.A. van Cappellen ◽  
Harm H. Kampinga ◽  
...  
Keyword(s):  
Dna Damage ◽  
Chinese Hamster Ovary ◽  
Mammalian Cells ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Dna Integrity ◽  
Ovary Cells ◽  
Daughter Cells ◽  
Multipolar Spindles ◽  
Drosophila Embryos

A well-established function of centrosomes is their role in accomplishing a successful mitosis that gives rise to a pair of identical daughter cells. We recently showed that DNA replication defects and DNA damage in Drosophila embryos trigger centrosomal changes, but it remained unclear whether comparable centrosomal responses can be provoked in somatic mammalian cells. To investigate the centrosomal organization in the presence of impaired DNA integrity, live and ultrastructural analysis was performed on γ-tubulin–GFP and EGFP–α-tubulin–expressing Chinese hamster ovary cells. We have shown that during mitosis in the presence of incompletely replicated or damaged DNA, centrosomes split into fractions containing only one centriole. This results in the formation of multipolar spindles with extra centrosome-like structures. Despite the extra centrosomes and the multipolarity of the spindles, cells do exit from mitosis, resulting in severe division errors. Our data provide evidence of a novel mechanism showing how numerous centrosomes and spindle defects can arise and how this can lead to the formation of aneuploid cells.

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