Cardioteratogenic dose of ethanol reduces both lactic dehydrogenase and succinic dehydrogenase activity in the bulbar ridges of the embryonic chick heart

1994 ◽  
Vol 14 (1) ◽  
pp. 27-31 ◽  
Author(s):  
Harold J. Bruyere ◽  
Charles E. Stith ◽  
Thomas A. Thorn
Keyword(s):  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Lactic Dehydrogenase ◽  
Embryonic Chick ◽  
Succinic Dehydrogenase Activity ◽  
Chick Heart ◽  
Embryonic Chick Heart

scholarly journals AN OSMIOPHILIC DISTYRYL DITETRAZOLIUM SALT (DS-NBT) FOR ULTRASTRUCTURAL DEHYDROGENASE ACTIVITY

1971 ◽  
Vol 19 (5) ◽  
pp. 273-285 ◽  
Author(s):  
ARNOLD M. SELIGMAN ◽  
IZHAK NIR ◽  
ROBERT E. PLAPINGER
Keyword(s):  
Skeletal Muscle ◽  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Lactic Dehydrogenase ◽  
Structural Features ◽  
Rat Skeletal Muscle ◽  
Succinic Dehydrogenase Activity ◽  
Tissue Penetration ◽  
Diaphorase Activity ◽  
Doubly Charged

The preparation of a new osmiophilic ditetrazolium salt (DS-NBT) containing two styryl groups, but retaining the essential structural features of nitro-BT, is given. DS-NBT may be used to demonstrate succinic dehydrogenase activity (SDH) faster than with nitro-BT and fully as rapidly as with TC-NBT. DS-NBT is more stable than TC-NBT and it is more reliably and more readily osmicated than TC-NBT. The ultrastructural sites of SDH and NADH2 diaphorase activity are similar to those shown for cytochrome oxidase activity with diaminobenzidine in rat heart. The sites of lactic dehydrogenase activity are found in the sarcoplasmic reticulum and mitochondria of rat skeletal muscle fixed in formaldehyde for 3 hr. Tissue penetration of the doubly charged ditetrazolium salt (DS-NBT) was facilitated by 40-sec sonication and by 5% dimethyl sulfoxide. Illustrative examples and controls are included.

Acetylcholinesterase-acetylcholine, an enzyme system essential to rhythmicity in the preneural embryonic chick heart

1966 ◽  
Vol 154 (3) ◽  
pp. 675-683 ◽  
Author(s):  
George Hugo Paff ◽  
Robert Joseph Boucek ◽  
Thorne Parsons Glander
Keyword(s):  
Enzyme System ◽  
Embryonic Chick ◽  
Chick Heart ◽  
Embryonic Chick Heart
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