In vitro antioxidant and antigenotoxic potentials of 3,5-O-di-galloylquinic acid extracted from Myrtus communis leaves and modulation of cell gene expression by H2O2

2011 ◽  
Vol 32 (5) ◽  
pp. 333-341 ◽  
Author(s):  
Skandrani Ines ◽  
Bouhlel Ines ◽  
Bhouri Wissem ◽  
Ben Sghaier Mohamed ◽  
Hayder Nawel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Myrtus Communis ◽  
Cell Gene Expression ◽  
Cell Gene

Selective and transient in vitro effects of heat shock on alveolar type II cell gene expression

1997 ◽  
Vol 272 (1) ◽  
pp. L132-L138 ◽  
Author(s):  
H. R. Wong ◽  
M. Ryan ◽  
S. Gebb ◽  
J. R. Wispe
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Alveolar Type ◽  
Type Ii ◽  
Alveolar Type Ii Cell ◽  
Cell Gene Expression ◽  
Type Ii Cell ◽  
In Vitro Effects ◽  
Cell Gene

The heat shock response is a highly conserved stress response that can transiently inhibit non-heat shock protein gene expression. Although heat shock protects against acute lung injury, its effects on lung cell gene expression are not known. We studied the in vitro effects of heat shock on the expression of several genes important to alveolar type II cells. Prior induction of heat shock transiently inhibited cytokine-mediated inducible nitric oxide synthase gene expression and cytokine-mediated manganese-superoxide dismutase mRNA expression in murine lung epithelium. In contrast, heat shock had no effect on expression of surfactant protein (SP) A or B mRNA, or SP-B peptide synthesis. Cell survival studies indicated that the inhibitory effects were not secondary to cytotoxicity. Previous heat shock also modestly enhanced the ability of cells to withstand oxidant stress. We conclude that in vitro heat shock has selective and transient inhibitory effects on alveolar type II cell gene expression. Transient inhibition of cytokine-inducible genes, with concomitant conservation of genes required for normal respiratory function (SP) may explain, in part, the mechanism by which heat shock protects during acute lung injury.

scholarly journals Oxygen-regulated gene expression in murine cumulus cells

2015 ◽  
Vol 27 (2) ◽  
pp. 407 ◽  
Author(s):  
Karen L. Kind ◽  
Kimberley K. Y. Tam ◽  
Kelly M. Banwell ◽  
Ashley D. Gauld ◽  
Darryl L. Russell ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Function ◽  
Ovarian Follicle ◽  
Cumulus Cell ◽  
Cumulus Cells ◽  
Glucose Transporter 1 ◽  
Cell Gene Expression ◽  
Regulated Gene Expression ◽  
Cell Gene

Oxygen is an important component of the environment of the cumulus–oocyte complex (COC), both in vivo within the ovarian follicle and during in vitro oocyte maturation (IVM). Cumulus cells have a key role in supporting oocyte development, and cumulus cell function and gene expression are known to be altered when the environment of the COC is perturbed. Oxygen-regulated gene expression is mediated through the actions of the transcription factors, the hypoxia-inducible factors (HIFs). In the present study, the effect of oxygen on cumulus cell gene expression was examined following in vitro maturation of the murine COC at 2%, 5% or 20% oxygen. Increased expression of HIF-responsive genes, including glucose transporter-1, lactate dehydrogenase A and BCL2/adenovirus E1B interacting protein 3, was observed in cumulus cells matured at 2% or 5%, compared with 20% oxygen. Stabilisation of HIF1α protein in cumulus cells exposed to low oxygen was confirmed by western blot and HIF-mediated transcriptional activity was demonstrated using a transgenic mouse expressing green fluorescent protein under the control of a promoter containing hypoxia response elements. These results indicate that oxygen concentration influences cumulus cell gene expression and support a role for HIF1α in mediating the cumulus cell response to varying oxygen.

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