Different effects of polycyclic aromatic hydrocarbons in artificial and in environmental mixtures on the free living nematode C. elegans

2011 ◽  
Vol 32 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Vania Cosma Liuzzi ◽  
Barbara Elisabetta Daresta ◽  
Gianluigi Gennaro ◽  
Carla De Giorgi
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Free Living ◽  
C Elegans ◽  
Free Living Nematode ◽  
Polycyclic Aromatic ◽  
Environmental Mixtures

Transformation of chrysene and other polycyclic aromatic hydrocarbon mixtures by the fungus Cunninghamella elegans

1995 ◽  
Vol 73 (S1) ◽  
pp. 1025-1033 ◽  
Author(s):  
Jairaj V. Pothuluri ◽  
Allison Selby ◽  
Frederick E. Evans ◽  
James P. Freeman ◽  
Carl E. Cerniglia
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbons ◽  
High Performance ◽  
Parent Compound ◽  
Environmental Pollutants ◽  
Health Concern ◽  
Cunninghamella Elegans ◽  
C Elegans ◽  
Polycyclic Aromatic

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous and persistent environmental pollutants; some are mutagenic, toxic, and carcinogenic and remain a public health concern. We investigated the metabolism of mixtures of PAHs and a tetracyclic aromatic hydrocarbon, chrysene, by the filamentous fungus, Cunninghamella elegans ATCC 36112. Cunninghamella elegans metabolized a mixture of PAHs including the carcinogen benzo[a]pyrene, phenanthrene, fluoranthene, pyrene, and acenaphthene completely to hydroxylated intermediates within 24 h. The metabolites from the PAH mixtures were similar to those formed in earlier studies of individual PAH compounds. In separate experiments with chrysene, C. elegans metabolized about 45% of the [5,6,11,12-14C]chrysene added to cultures during 144 h incubation. The two major metabolites of chrysene were separated by reverse-phase high performance liquid chromatography and identified by ultraviolet–visible, mass spectral, and 1H-nuclear magnetic resonance techniques as sulfate conjugates of 2,8-dihydroxychrysene and 2-hydroxychrysene. The two major metabolites accounted for about 33% of the total metabolism. The formation of sulfate conjugates of phenolic chrysene metabolites and glucoside conjugates and hydroxylated products of PAH mixtures by C. elegans may be a detoxification step, because these types of products are generally less toxic than the parent compound. Key words: polycyclic aromatic hydrocarbons, PAH mixtures, chrysene, Cunninghamella elegans, biotransformation, oxidation.

scholarly journals Polycyclic aromatic hydrocarbons as skin carcinogens: Comparison of benzo[a]pyrene, dibenzo[def,p]chrysene and three environmental mixtures in the FVB/N mouse

2012 ◽  
Vol 264 (3) ◽  
pp. 377-386 ◽  
Author(s):  
Lisbeth K. Siddens ◽  
Andrew Larkin ◽  
Sharon K. Krueger ◽  
Christopher A. Bradfield ◽  
Katrina M. Waters ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Polycyclic Aromatic ◽  
Environmental Mixtures

A comparison of the response of twoBurkholderia fungorumstrains grown as planktonic cells versus biofilm to dibenzothiophene and select polycyclic aromatic hydrocarbons

2016 ◽  
Vol 62 (10) ◽  
pp. 851-860 ◽  
Author(s):  
Nazanin Seyed Khoei ◽  
Marco Andreolli ◽  
Silvia Lampis ◽  
Giovanni Vallini ◽  
Raymond J. Turner
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Laser Scanning ◽  
Living Cells ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Planktonic Cells ◽  
Free Living ◽  
Polycyclic Aromatic ◽  
High Concentrations

In natural environments, bacteria often exist in close association with surfaces and interfaces by establishing biofilms. Here, we report on the ability of Burkholderia fungorum strains DBT1 and 95 to survive in high concentrations of hydrocarbons, and we compare their growth as a biofilm vs. planktonic cells. The 2 compounds tested were dibenzothiophene (DBT) and a mixture of naphthalene, phenanthrene, and pyrene (5:2:1) as representative compounds of thiophenes and polycyclic aromatic hydrocarbons (PAHs), respectively. The results showed that both strains were able to degrade DBT and to survive in the presence of up to a 2000 mg·L−1concentration of this compound both as a biofilm and as free-living cells. Moreover, B. fungorum DBT1 showed reduced tolerance towards the mixed PAHs (2000 mg·L−1naphthalene, 800 mg·L−1phenanthrene, and 400 mg·L−1pyrene) both as a biofilm and as free-living cells. Conversely, biofilms of B. fungorum 95 enhanced resistance against these toxic compounds compared with planktonic cells (P < 0.05). Visual observation through confocal laser scanning microscopy showed that exposure of biofilms to DBT and PAHs altered their structure: high concentrations of DBT triggered an aggregation of biofilm cells. These findings provide new perspectives on the effectiveness of using DBT-degrading bacterial strains in bioremediation of hydrocarbon-contaminated sites.

scholarly journals BIORREMOÇÃO DE FENANTRENO POR BIOMASSA VIVA E INATIVADA CUNNINGHAMELLA ELEGANS UCP0542

e-xacta ◽  
2013 ◽  
Vol 6 (1) ◽  
pp. 1 ◽  
Author(s):  
Marta Cristina Freitas Silva ◽  
Adriana Almeida Antunes ◽  
Clarissa Isabel Matos Lins ◽  
Antonio Helder Parente ◽  
Sônia Valéria Pereira ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Cunninghamella Elegans ◽  
Biotechnological Potential ◽  
Independent Variables ◽  
C Elegans ◽  
Live Biomass ◽  
Polycyclic Aromatic ◽  
Full Factorial ◽  
Inactivated Biomass

<p align="justify">Realizou-se estudos avaliando a ação da biomassa viva e inativada de Cunninghamella elegans crescida, visando à remoção do hidrocarboneto aromático policíclico fenantreno. Monitorou-se o processo de remoção do fenantreno a cada 24 h por espectrofotometria, de acordo com um planejamento fatorial completo de 22 e, como variáveis independentes 0,1 e 0,2 mM de fenantreno, na presença de 0,6, 3,3 e 6% de glicose e variável resposta o conteúdo de fenantreno removido. Os resultados obtidos indicaram que tanto a biomassa inativada como viva removeram, respectivamente, 90,0% e 82,0% a concentração de 0,2mM de fenantreno na presença da menor concentração de glicose (0,6%). A biomassa inativada de C. elegans demonstrou ser um sorbente com elevado potencial biotecnológico para a remediação de áreas poluídas com hidrocarbonetos aromáticos policíclicos.</p><p align="justify">Abstract</p><p align="justify">Studies were carried out evaluating the action of the life and inactivated biomass of Cunninghamella elegans grown to removal polycyclic aromatic hydrocarbon phenanthrene. The removal process was monitored each 24h by spectrophotometry, de acordo com according to full factorial design 2 2 , and independent variables the phenanthrene concentrations [0.1 and 0.2 mM], and the content of phenanthrene removed as variable response.The results showed that both inactivated and live biomass from C. elegans removed 85.0% and 90.0%, respectively, of phenantrene of 0.2 mM on glucose (0.6) concentration. The inactivated biomass from C. elegans shows a sorbent with high biotechnological potential for bioremediation of polluted area with polycyclic aromatic hydrocarbons.</p>

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