Study on the in vitro effect of zearalenone andα-zearalenol on boar sperm–zona pellucida interaction by hemizona assay application

2007 ◽  
Vol 27 (5) ◽  
pp. 498-505 ◽  
Author(s):  
I. A. Tsakmakidis ◽  
A. G. Lymberopoulos ◽  
E. Vainas ◽  
C. M. Boscos ◽  
S. C. Kyriakis ◽  
...  
Keyword(s):  
Zona Pellucida ◽  
Boar Sperm ◽  
Vitro Effect ◽  
Hemizona Assay

Native zona pellucida reactivity and in-vitro effect on human sperm–egg binding with antisera against bonnet monkey ZP1 and ZP3 synthetic peptides

2002 ◽  
Vol 56 (1-2) ◽  
pp. 77-91 ◽  
Author(s):  
Neela Sivapurapu ◽  
Abhishek Upadhyay ◽  
Akiko Hasegawa ◽  
Koji Koyama ◽  
Satish K Gupta
Keyword(s):  
Zona Pellucida ◽  
Synthetic Peptides ◽  
Human Sperm ◽  
Bonnet Monkey ◽  
Vitro Effect

In vitro Effect of Zearalenone and ?-Zearalenol on Boar Sperm Characteristics and Acrosome Reaction

2006 ◽  
Vol 41 (5) ◽  
pp. 394-401 ◽  
Author(s):  
IA Tsakmakidis ◽  
AG Lymberopoulos ◽  
C Alexopoulos ◽  
CM Boscos ◽  
SC Kyriakis
Keyword(s):  
Acrosome Reaction ◽  
Sperm Characteristics ◽  
Boar Sperm ◽  
Vitro Effect

scholarly journals ACRBP (Sp32) is involved in priming sperm for the acrosome reaction and the binding of sperm to the zona pellucida in a porcine model

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0251973
Author(s):  
Yoku Kato ◽  
Satheesh Kumar ◽  
Christian Lessard ◽  
Janice L. Bailey
Keyword(s):  
Endoplasmic Reticulum ◽  
Zona Pellucida ◽  
Acrosome Reaction ◽  
Porcine Model ◽  
Sperm Head ◽  
Mouse Sperm ◽  
Phosphorylated Proteins ◽  
Boar Sperm

In boar sperm, we have previously shown that capacitation is associated with the appearance of the p32 tyrosine phosphoprotein complex. The principal tyrosine phosphoprotein involved in this complex is the acrosin-binding protein (ACRBP), which regulates the autoconversion of proacrosin to intermediate forms of acrosin in both boar and mouse sperm. However, the complete biological role of ACRBP has not yet been elucidated. In this study, we tested the hypothesis that tyrosine phophorylation and the presence of the ACRBP in the sperm head are largely necessary to induce capacitation, the acrosome reaction (AR) and sperm-zona pellucida (ZP) binding, all of which are necessary steps for fertilization. In vitro fertilization (IVF) was performed using matured porcine oocytes and pre-capacitated boar sperm cultured with anti-phosphotyrosine antibodies or antibodies against ACRBP. Anti-ACRBP antibodies reduced capacitation and spontaneous AR (P<0.05). Sperm-ZP binding declined in the presence of anti-phosphotyrosine or anti-ACRBP antibodies. The localisation of anti-ACRBP antibodies on the sperm head, reduced the ability of the sperm to undergo the AR in response to solubilized ZP or by inhibiting the sarco/endoplasmic reticulum Ca2+-ATPase. These results support our hypothesis that tyrosine phosphorylated proteins and ACRBP are present upon the sperm surface in order to participate in sperm-ZP binding, and that ACRBP upon the surface of the sperm head facilitates capacitation and the AR in the porcine.

scholarly journals Immunoreactivity and in-vitro effect on human sperm-egg binding of antibodies against peptides corresponding to bonnet monkey zona pellucida-3 glycoprotein

1997 ◽  
Vol 12 (12) ◽  
pp. 2664-2670 ◽  
Author(s):  
A. Afzalpurkar ◽  
H. Shibahara ◽  
A. Hasegawa ◽  
K. Koyama ◽  
S. K. Gupta
Keyword(s):  
Zona Pellucida ◽  
Human Sperm ◽  
Bonnet Monkey ◽  
Vitro Effect

In vitro effect of an aqueous extract of Artocarpus lakoocha on the intestinal parasites in cattle

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
N Saowakon ◽  
P Chaichanasak ◽  
C Wanichanon ◽  
V Reutrakul ◽  
P Sobhon
Keyword(s):  
Aqueous Extract ◽  
Intestinal Parasites ◽  
Vitro Effect

In vitro effect of purified plumbagin of Plumbago indica against motility of Paramphistomum cervi

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
N Saowakon ◽  
P Kueakhai ◽  
N Changklungmoa ◽  
N Lorsuwannarat ◽  
P Sobhon
Keyword(s):  
Plumbago Indica ◽  
Vitro Effect ◽  
Paramphistomum Cervi

In vitro effect on myometrial contractility by a combination of Bryophyllum pinnatum juice and atosiban

2017 ◽  
Author(s):  
S Santos ◽  
C Haslinger ◽  
M Hamburger ◽  
M Mennet ◽  
O Potterat ◽  
...  
Keyword(s):  
Myometrial Contractility ◽  
Vitro Effect ◽  
Bryophyllum Pinnatum

In vitro effect of prolactin on the osteogenic potential of bone marrow mesenchymal stem cells of rats

2013 ◽  
Author(s):  
Melo Ocarino Natalia de ◽  
Silvia Silva Santos ◽  
Lorena Rocha ◽  
Juneo Freitas ◽  
Reis Amanda Maria Sena ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Potential ◽  
Marrow Mesenchymal Stem Cells ◽  
Vitro Effect

Differences between antigenic determinants of pig and cat zona pellucida proteins

Reproduction ◽  
2000 ◽  
pp. 15-23 ◽  
Author(s):  
K Jewgenow ◽  
M Rohleder ◽  
I Wegner
Keyword(s):  
In Vitro Fertilization ◽  
Zona Pellucida ◽  
Antigenic Determinants ◽  
Vitro Fertilization ◽  
Contraceptive Vaccine ◽  
Sperm Binding ◽  
Dependent Inhibition ◽  
Dose Dependent Inhibition ◽  
Dose Dependent

Despite many efforts, the control of reproduction in feral cat populations is still a problem in urban regions around the world. Immunocontraception is a promising approach; thus the present study examined the suitability of the widely used pig zona pellucida proteins (pZP) for contraception in feral domestic cats. Purified zona pellucida proteins obtained from pig and cat ovaries were used to produce highly specific antisera in rabbits. Antibodies against pZP raised in rabbits or lions were not effective inhibitors of either in vitro sperm binding (cat spermatozoa to cat oocytes) or in vitro fertilization in cats, whereas antibodies against feline zona pellucida proteins (fZP) raised in rabbits showed a dose-dependent inhibition of in vitro fertilization. Immunoelectrophoresis, ELISA and immunohistology of ovaries confirmed these results, showing crossreactivity of anti-fZP sera to fZP and to a lesser extent to pZP, but no interaction of anti-pZP sera with fZP. It is concluded that cat and pig zonae pellucidae express a very small number of shared antigenic determinants, making the use of pZP vaccine in cats questionable. A contraceptive vaccine based on feline zona pellucida determinants will be a better choice for the control of reproduction in feral cats if immunogenity can be achieved.

Effect of ornidazole on fertility of male rats: inhibition of a glycolysis-related motility pattern and zona binding required for fertilization in vitro

Reproduction ◽  
2000 ◽  
pp. 127-135 ◽  
Author(s):  
W Bone ◽  
NG Jones ◽  
G Kamp ◽  
CH Yeung ◽  
TG Cooper
Keyword(s):  
Zona Pellucida ◽  
Glucose Utilization ◽  
Cumulus Cells ◽  
Glycolytic Enzyme ◽  
Male Rats ◽  
Fertilization In Vitro ◽  
Swimming Pattern ◽  
Principal Piece ◽  
Free Media

The effects of the male antifertility agent ornidazole on glycolysis as a prerequisite for fertilization were investigated in rats. Antifertility doses of ornidazole inhibited glycolysis within mature spermatozoa as determined from the lack of glucose utilization, reduced acidosis under anaerobic conditions and reduced glycolytic enzyme activity. As a consequence, cauda epididymidal spermatozoa from ornidazole-fed rats were unable to fertilize rat oocytes in vitro, with or without cumulus cells, which was not due to transfer of an inhibitor in epididymal fluid with the spermatozoa. Under IVF conditions, binding to the zona pellucida was reduced in spermatozoa from ornidazole-fed males and the spermatozoa did not undergo a change in swimming pattern, which was observed in controls. The block to fertilization could be explained by the disruption of glycolysis-dependent events, since reduced binding to the zona pellucida and a lack of kinematic changes were demonstrated by control spermatozoa in glucose-free media in the presence of respiratory substrates. The importance of glycolysis for binding to, and penetration of, the zona pellucida, and hyperactivation in rats is discussed in relation to the glycolytic production of ATP in the principal piece in which local deprivation of energy may explain the reduced force of spermatozoa from ornidazole-fed males.

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