scholarly journals Mutational landscape of receptor guanylyl cyclase C: Functional analysis and disease‐related mutations

IUBMB Life ◽  
2020 ◽  
Vol 72 (6) ◽  
pp. 1145-1159
Author(s):  
Avipsa Bose ◽  
Sanghita Banerjee ◽  
Sandhya S. Visweswariah
Keyword(s):  
Functional Analysis ◽  
Guanylyl Cyclase ◽  
Guanylyl Cyclase C ◽  
Mutational Landscape

scholarly journals In Silico Prediction, Molecular Docking and Dynamics Studies of Steroidal Alkaloids of Holarrhena pubescens Wall. ex G. Don to Guanylyl Cyclase C: Implications in Designing of Novel Antidiarrheal Therapeutic Strategies

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4147
Author(s):  
Neha Gupta ◽  
Saurav Kumar Choudhary ◽  
Neeta Bhagat ◽  
Muthusamy Karthikeyan ◽  
Archana Chaturvedi
Keyword(s):  
Molecular Docking ◽  
Amino Acid ◽  
Guanylyl Cyclase ◽  
Extracellular Domain ◽  
Enterotoxigenic Escherichia Coli ◽  
Watery Diarrhea ◽  
Guanylyl Cyclase C ◽  
Amino Acid Residues ◽  
Lead Compounds

The binding of heat stable enterotoxin (STa) secreted by enterotoxigenic Escherichia coli (ETEC) to the extracellular domain of guanylyl cyclase c (ECDGC-C) causes activation of a signaling cascade, which ultimately results in watery diarrhea. We carried out this study with the objective of finding ligands that would interfere with the binding of STa on ECDGC-C. With this view in mind, we tested the biological activity of a alkaloid rich fraction of Holarrhena pubescens against ETEC under in vitro conditions. Since this fraction showed significant antibacterial activity against ETEC, we decided to test the screen binding affinity of nine compounds of steroidal alkaloid type from Holarrhena pubescens against extracellular domain (ECD) by molecular docking and identified three compounds with significant binding energy. Molecular dynamics simulations were performed for all the three lead compounds to establish the stability of their interaction with the target protein. Pharmacokinetics and toxicity profiling of these leads demonstrated that they possessed good drug-like properties. Furthermore, the ability of these leads to inhibit the binding of STa to ECD was evaluated. This was first done by identifying amino acid residues of ECDGC-C binding to STa by protein–protein docking. The results were matched with our molecular docking results. We report here that holadysenterine, one of the lead compounds that showed a strong affinity for the amino acid residues on ECDGC-C, also binds to STa. This suggests that holadysenterine has the potential to inhibit binding of STa on ECD and can be considered for future study, involving its validation through in vitro assays and animal model studies.

Identification of a binding region onEscherichia coli heat-stable enterotoxin to intestinal guanylyl cyclase C

1997 ◽  
Vol 4 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Makoto Hasegawa ◽  
Yuki Kawano ◽  
Kazuya Matsumoto ◽  
Yuji Hidaka ◽  
Takashi Sato ◽  
...  
Keyword(s):  
Guanylyl Cyclase ◽  
Guanylyl Cyclase C ◽  
Binding Region ◽  
Heat Stable

Evaluation of Guanylyl Cyclase C Lymph Node Status for Colon Cancer Staging and Prognosis

2011 ◽  
Vol 18 (12) ◽  
pp. 3261-3270 ◽  
Author(s):  
Daniel J. Sargent ◽  
Murray B. Resnick ◽  
Michael O. Meyers ◽  
Atoussa Goldar-Najafi ◽  
Thomas Clancy ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Lymph Node ◽  
Guanylyl Cyclase ◽  
Cancer Staging ◽  
Lymph Node Status ◽  
Guanylyl Cyclase C ◽  
Node Status

scholarly journals A Novel PDZ Protein Regulates the Activity of Guanylyl Cyclase C, the Heat-stable Enterotoxin Receptor

2002 ◽  
Vol 277 (25) ◽  
pp. 22934-22941 ◽  
Author(s):  
Robert O. Scott ◽  
William R. Thelin ◽  
Sharon L. Milgram
Keyword(s):  
Guanylyl Cyclase ◽  
Guanylyl Cyclase C ◽  
Heat Stable ◽  
Pdz Protein

scholarly journals Guanylyl cyclase C and guanylin reduce fat droplet accumulation in cattle mesenteric adipose tissue

2017 ◽  
Vol 18 (3) ◽  
pp. 341
Author(s):  
Masahiro Yasuda ◽  
Jyunya Kawabata ◽  
Sayaka Akieda-Asai ◽  
Tetsuo Nasu ◽  
Yukari Date
Keyword(s):  
Adipose Tissue ◽  
Guanylyl Cyclase ◽  
Guanylyl Cyclase C ◽  
Mesenteric Adipose Tissue

scholarly journals Expression of guanylyl cyclase C in tissue samples and the circulation of rectal cancer patients

Oncotarget ◽  
2017 ◽  
Vol 8 (24) ◽  
pp. 38841-38849 ◽  
Author(s):  
Yong Liu ◽  
Guoping Cheng ◽  
Jun Qian ◽  
HaiXing Ju ◽  
YuPing Zhu ◽  
...  
Keyword(s):  
Rectal Cancer ◽  
Cancer Patients ◽  
Guanylyl Cyclase ◽  
Guanylyl Cyclase C ◽  
Tissue Samples

Guanylyl Cyclase C Messenger RNA Is a Biomarker for Recurrent Stage II Colorectal Cancer

1999 ◽  
Vol 131 (11) ◽  
pp. 805 ◽  
Author(s):  
Burt Cagir ◽  
Alyssa Gelmann ◽  
Jason Park ◽  
Tracy Fava ◽  
Alexander Tankelevitch ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Guanylyl Cyclase ◽  
Messenger Rna ◽  
Stage Ii ◽  
Guanylyl Cyclase C ◽  
Stage Ii Colorectal Cancer
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