scholarly journals Gene and protein delivery using four cell penetrating peptides for HIV‐1 vaccine development

IUBMB Life ◽  
2019 ◽  
Vol 71 (10) ◽  
pp. 1619-1633 ◽  
Author(s):  
Bahareh Rostami ◽  
Shiva Irani ◽  
Azam Bolhassani ◽  
Reza Ahangari Cohan
Keyword(s):  
Protein Delivery ◽  
Vaccine Development ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating ◽  
Hiv 1

scholarly journals M918: A Novel Cell Penetrating Peptide for Effective Delivery of HIV-1 Nef and Hsp20-Nef Proteins into Eukaryotic Cell Lines

2019 ◽  
Vol 16 (4) ◽  
pp. 280-287 ◽  
Author(s):  
Bahareh Rostami ◽  
Shiva Irani ◽  
Azam Bolhassani ◽  
Reza Ahangari Cohan
Keyword(s):  
Cell Line ◽  
Mammalian Cells ◽  
Vaccine Development ◽  
Expression System ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Transfected Cells ◽  
Dominant Band ◽  
Cell Penetrating ◽  
Hiv 1

Background: HIV-1 Nef protein is a possible attractive target in the development of therapeutic HIV vaccines including protein-based vaccines. The most important disadvantage of protein-based vaccines is their low immunogenicity which can be improved by heat shock proteins (Hsps) as an immunomodulator, and cell-penetrating peptides (CPPs) as a carrier. Methods: In this study, the HIV-1 Nef and Hsp20-Nef proteins were generated in E.coli expression system for delivery into the HEK-293T mammalian cell line using a novel cell-penetrating peptide, M918, in a non-covalent fashion. The size, zeta potential and morphology of the peptide/protein complexes were studied by scanning electron microscopy (SEM) and Zeta sizer. The efficiency of Nef and Hsp20-Nef transfection using M918 was evaluated by western blotting in HEK-293T cell line. Results: The SEM data confirmed the formation of discrete nanoparticles with a diameter of approximately 200-250 nm and 50-80 nm for M918/Nef and M918/Hsp20-Nef, respectively. The dominant band of ~ 27 kDa and ~ 47 kDa was detected in the transfected cells with the Nef/ M918 and Hsp20-Nef/ M918 nanoparticles at a molar ratio of 1:20 using anti-HIV-1 Nef monoclonal antibody. These bands were not detected in the un-transfected and transfected cells with Nef or Hsp20- Nef protein alone indicating that M918 could increase the penetration of Nef and Hsp20-Nef proteins into the cells. Conclusion: These data suggest that M918 CPP can be used to enter HIV-1 Nef and Hsp20-Nef proteins inside mammalian cells efficiently as a promising approach in HIV-1 vaccine development.

Cationic Cell Penetrating Peptides as Potential Inhibitors of HIV-1 Infection

2012 ◽  
Vol 9 (1) ◽  
pp. S94
Author(s):  
Shawn Keogan ◽  
Shendra Passic ◽  
Brian Wigdahl ◽  
Fred Krebs
Keyword(s):  
Cell Penetrating Peptides ◽  
Cell Penetrating ◽  
Potential Inhibitors ◽  
Hiv 1

scholarly journals Design and in vitro delivery of HIV-1 multi-epitope DNA and peptide constructs using novel cell-penetrating peptides

2019 ◽  
Vol 41 (11) ◽  
pp. 1283-1298 ◽  
Author(s):  
Saba Davoodi ◽  
Azam Bolhassani ◽  
Seyed Mehdi Sadat ◽  
Shiva Irani
Keyword(s):  
Cell Penetrating Peptides ◽  
Cell Penetrating ◽  
Hiv 1

scholarly journals Protein Delivery of Cell-Penetrating Zinc-Finger Activators Stimulates Latent HIV-1-Infected Cells

2020 ◽  
Vol 18 ◽  
pp. 145-158
Author(s):  
Pedro R.L. Perdigão ◽  
Catarina Cunha-Santos ◽  
Carlos F. Barbas ◽  
Mariana Santa-Marta ◽  
Joao Goncalves
Keyword(s):  
Zinc Finger ◽  
Protein Delivery ◽  
Cell Penetrating ◽  
Infected Cells ◽  
Latent Hiv ◽  
Hiv 1

scholarly journals The many futures for cell-penetrating peptides: how soon is now?

2007 ◽  
Vol 35 (4) ◽  
pp. 767-769 ◽  
Author(s):  
J. Howl ◽  
I.D. Nicholl ◽  
S. Jones
Keyword(s):  
Positive Impact ◽  
Biochemical Properties ◽  
Cell Penetrating Peptides ◽  
Protein Transduction Domains ◽  
Molecular Therapeutics ◽  
Cargo Delivery ◽  
Cell Penetrating ◽  
Transactivator Of Transcription ◽  
The Many ◽  
Hiv 1

Studies of CPPs (cell-penetrating peptides), sequences that are also commonly designated as protein transduction domains, now extend to a second decade of exciting and far-reaching discoveries. CPPs are proven vehicles for the intracellular delivery of macromolecules that include oligonucleotides, peptides and proteins, low-molecular-mass drugs, nanoparticles and liposomes. The biochemical properties of different classes of CPP, including various sequences derived from the HIV-1 Tat (transactivator of transcription) [e.g. Tat-(48–60), GRKKRRQRRRPPQ], and the homeodomain of the Drosophila homeoprotein Antennapaedia (residues 43–58, commonly named penetratin, RQIKIWFQNRRMKWKK), also provide novel insights into the fundamental mechanisms of translocation across biological membranes. Thus the efficacy of CPP-mediated cargo delivery continues to provide valuable tools for biomedical research and, as witnessed in 2007, candidate and emerging therapeutics. Thus it is anticipated that the further refinement of CPP technologies will provide drug-delivery vectors, cellular imaging tools, nanoparticulate devices and molecular therapeutics that will have a positive impact on the healthcare arena. The intention of this article is to provide both a succinct overview of current developments and applications of CPP technologies, and to illustrate key developments that the concerted efforts of the many researchers contributing to the Biochemical Society's Focused Meeting in Telford predict for the future. The accompanying papers in this issue of Biochemical Society Transactions provide additional details and appropriate references. Hopefully, the important and eagerly anticipated biomedical and clinical developments within the CPP field will occur sooner rather than later.

scholarly journals Inhibition of HIV-1 Replication by Cell-penetrating Peptides Binding Rev

2003 ◽  
Vol 279 (10) ◽  
pp. 9208-9214 ◽  
Author(s):  
Armelle Roisin ◽  
Jean-Philippe Robin ◽  
Nathalie Dereuddre-Bosquet ◽  
Anne-Laure Vitte ◽  
Dominique Dormont ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
Cell Penetrating ◽  
Hiv 1

Targeted Subcellular Protein Delivery Using Cleavable Cyclic Cell-Penetrating Peptides

2019 ◽  
Vol 30 (2) ◽  
pp. 400-404 ◽  
Author(s):  
Anselm F. L. Schneider ◽  
Antoine L. D. Wallabregue ◽  
Luise Franz ◽  
Christian P. R. Hackenberger
Keyword(s):  
Protein Delivery ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating
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