How Catalytic Order Drives the Complexification of Molecular Replication Networks

2015 ◽  
Vol 55 (8) ◽  
pp. 880-890 ◽  
Author(s):  
Nathaniel Wagner ◽  
Gonen Ashkenasy
Keyword(s):  
Molecular Replication

Molecular Replication: From Minimal to Complex Systems

1994 ◽  
pp. 45-64
Author(s):  
D. Sievers ◽  
T. Achilles ◽  
J. Burmeister ◽  
S. Jordan ◽  
A. Terfort ◽  
...  
Keyword(s):  
Complex Systems ◽  
Molecular Replication

scholarly journals Molecular Replication

1985 ◽  
Vol 112 ◽  
pp. 199-200
Author(s):  
Leslie E. Orgel
Keyword(s):  
Protein Synthesis ◽  
Genetic Information ◽  
Life Form ◽  
Similar Process ◽  
The Earth ◽  
Early Appearance ◽  
Living Organisms ◽  
The One ◽  
Molecular Replication

The replication of DNA is the process by means of which genetic information is passed from one generation to the next in all living organisms. It is widely believed that a similar process must have become important early in the development of life on the Earth, either before or at the same time as the evolution of protein synthesis. The evolution of any life-form based on familiar chemistry would seem to require the early appearance of some type of residue-by-residue replication, although it might be very different in detail from the one we know.

scholarly journals Probing of molecular replication and accumulation in shallow heat gradients through numerical simulations

2016 ◽  
Vol 18 (30) ◽  
pp. 20153-20159 ◽  
Author(s):  
Lorenz Keil ◽  
Michael Hartmann ◽  
Simon Lanzmich ◽  
Dieter Braun
Keyword(s):  
Numerical Simulations ◽  
Temperature Gradients ◽  
Porous Rocks ◽  
Highly Efficient ◽  
Temperature Oscillations ◽  
Molecular Replication

Shallow temperature gradients across porous rocks drive highly efficient molecular accumulation processes while simultaneously subjecting them to frequent temperature oscillations.

ChemInform Abstract: Studies in Molecular Replication

ChemInform ◽  
2010 ◽  
Vol 25 (49) ◽  
pp. no-no
Author(s):  
E. A. WINTNER ◽  
M. M. CONN ◽  
J. JUN. REBEK
Keyword(s):  
Molecular Replication

scholarly journals tRNA sequences can assemble into a replicator

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Alexandra Kühnlein ◽  
Simon A Lanzmich ◽  
Dieter Braun
Keyword(s):  
Genetic Code ◽  
Self Assembly ◽  
Molecular Assembly ◽  
Transfer Rna ◽  
Binary Sequences ◽  
Replication Fidelity ◽  
Dna Hairpins ◽  
Single Mechanism ◽  
Thermal Oscillations ◽  
Molecular Replication

Can replication and translation emerge in a single mechanism via self-assembly? The key molecule, transfer RNA (tRNA), is one of the most ancient molecules and contains the genetic code. Our experiments show how a pool of oligonucleotides, adapted with minor mutations from tRNA, spontaneously formed molecular assemblies and replicated information autonomously using only reversible hybridization under thermal oscillations. The pool of cross-complementary hairpins self-selected by agglomeration and sedimentation. The metastable DNA hairpins bound to a template and then interconnected by hybridization. Thermal oscillations separated replicates from their templates and drove an exponential, cross-catalytic replication. The molecular assembly could encode and replicate binary sequences with a replication fidelity corresponding to 85–90 % per nucleotide. The replication by a self-assembly of tRNA-like sequences suggests that early forms of tRNA could have been involved in molecular replication. This would link the evolution of translation to a mechanism of molecular replication.

scholarly journals Mutations in Hepatitis C Virus RNAs Conferring Cell Culture Adaptation

2001 ◽  
Vol 75 (3) ◽  
pp. 1437-1449 ◽  
Author(s):  
Volker Lohmann ◽  
Frank Körner ◽  
Aneta Dobierzewska ◽  
Ralf Bartenschlager
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Amino Acid ◽  
Colony Formation ◽  
Nonstructural Protein ◽  
Single Amino Acid ◽  
Gene Encoding ◽  
Adaptive Mutations ◽  
Drastic Effect ◽  
Molecular Replication

ABSTRACT As an initial approach to studying the molecular replication mechanisms of hepatitis C virus (HCV), a major causative agent of acute and chronic liver disease, we have recently developed selectable self-replicating RNAs. These replicons lacked the region encoding the structural proteins and instead carried the gene encoding the neomycin phosphotransferase. Although the replication levels of these RNAs within selected cells were high, the number of G418-resistant colonies was reproducibly low. In a search for the reason, we performed a detailed analysis of replicating HCV RNAs and identified several adaptive mutations enhancing the efficiency of colony formation by several orders of magnitude. Adaptive mutations were found in nearly every nonstructural protein but not in the 5′ or 3′ nontranslated regions. The most drastic effect was found with a single-amino-acid substitution in NS5B, increasing the number of colonies ∼500-fold. This mutation was conserved with RNAs isolated from one cell line, in contrast to other amino acid substitutions enhancing the efficiency of colony formation to a much lesser extent. Interestingly, some combinations of these nonconserved mutations with the highly adaptive one reduced the efficiency of colony formation drastically, suggesting that some adaptive mutations are not compatible.

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