scholarly journals Aberrant DNA methylation patterns in microsatellite stable human colorectal cancers define a new marker panel for the CpG island methylator phenotype

2021 ◽  
Author(s):  
Claudia Gebhard ◽  
Roger Mulet‐Lazaro ◽  
Dagmar Glatz ◽  
Lucia Schwarzfischer‐Pfeilschifter ◽  
Peter Schirmacher ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cpg Island ◽  
Colorectal Cancers ◽  
Cpg Island Methylator Phenotype ◽  
Marker Panel ◽  
Methylator Phenotype ◽  
Aberrant Dna Methylation ◽  
Methylation Patterns

Profiling of Aberrant DNA Methylation In AML Reveals Subclasses of CpG Islands with Epigenetic or Genetic Association

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 2498-2498
Author(s):  
Claudia Gebhard ◽  
Mohammed Sadeh ◽  
Dagmar Glatz ◽  
Lucia Schwarzfischer ◽  
Rainer Spang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
De Novo ◽  
Conflicts Of Interest ◽  
Cpg Island ◽  
Cpg Islands ◽  
Sequencing Data ◽  
Cpg Island Methylator Phenotype ◽  
Chip Sequencing ◽  
Aberrant Dna Methylation ◽  
Methylation Patterns

Abstract Abstract 2498 CpG islands show frequent and often disease-specific epigenetic alterations during malignant transformation, however, the underlying mechanisms are poorly understood. We used methyl-CpG immunoprecipitation (MCIp) to generate comparative DNA methylation profiles of 30 patients with acute myeloid leukemia for human CpG islands across the genome. DNA methylation profiles across 23.000 CpG islands revealed highly heterogeneous methylation patterns in AML with over 6000 CpG islands showing aberrant de novo methylation in AML. Based on these profiles we selected a subset of 380 CpG islands (covering 15.000 individual CpGs) for detailed fine-mapping analyses of aberrant DNA methylation in 185 patients with AML (50% normal karyotype). We found that a proportion of patients (5/185) displayed a concerted hypermethylation at almost all studied loci, representing the rare CpG island methylator phenotype (CIMP) in AML. Meta analysis of methylation profiling and published ChIP sequencing data separated CpG islands in two groups. A highly correlated subgroup of CpG island regions was strongly associated with histone H3 lysine 27 trimethylation in human hematopoietic progenitor cells, suggesting that disease-related de novo DNA methylation at these CpG islands is linked with polycomb group protein (PcG)-mediated repression. The group of mainly non-PcG target CpG islands showed heterogeneous methylation patterns across patients and unsupervised hierarchical clustering revealed a correlation of methylation profiles with genetic disease markers, including oncofusion proteins as well as CEBPA- and NPM1-mutations. Our study suggests that both epigenetic as well as genetic aberrations may underlay AML-related changes in CpG island DNA methylation states. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Ethnicity and Risk for Colorectal Cancers Showing Somatic BRAF V600E Mutation or CpG Island Methylator Phenotype

2008 ◽  
Vol 17 (7) ◽  
pp. 1774-1780 ◽  
Author(s):  
Dallas R. English ◽  
Joanne P. Young ◽  
Julie A. Simpson ◽  
Mark A. Jenkins ◽  
Melissa C. Southey ◽  
...  
Keyword(s):  
Cpg Island ◽  
Braf V600e Mutation ◽  
Braf V600e ◽  
Colorectal Cancers ◽  
Cpg Island Methylator Phenotype ◽  
Methylator Phenotype

scholarly journals 745 Colorectal Cancers (CRCs) With the CpG Island Methylator Phenotype (CIMP) Do Not Show Improved Survival With 5-Fluorouracil (5FU)-Based Adjuvant Chemotherapy

2010 ◽  
Vol 138 (5) ◽  
pp. S-102
Author(s):  
Rodrigo Jover ◽  
Thuy-Phuong T. Nguyen ◽  
Lucía Pérez-Carbonell ◽  
Artemio Payá ◽  
Cristina Alenda ◽  
...  
Keyword(s):  
Adjuvant Chemotherapy ◽  
Cpg Island ◽  
Colorectal Cancers ◽  
Cpg Island Methylator Phenotype ◽  
Methylator Phenotype

CpG Island Methylator Phenotype in Colorectal Cancers: Comparison of the New and Classic CpG Island Methylator Phenotype Marker Panels

2008 ◽  
Vol 132 (10) ◽  
pp. 1657-1665 ◽  
Author(s):  
Sun Lee ◽  
Nam-Yun Cho ◽  
Eun Joo Yoo ◽  
Jung Ho Kim ◽  
Gyeong Hoon Kang
Keyword(s):  
Clinical Outcome ◽  
Microsatellite Instability ◽  
Braf Mutation ◽  
Cpg Island ◽  
Tumor Location ◽  
Colorectal Cancers ◽  
Clinicopathologic Features ◽  
Cpg Island Methylator Phenotype ◽  
P Values ◽  
Methylator Phenotype

Abstract Context.—CpG island methylator phenotype (CIMP) designates a subset of colorectal cancers featuring concordant hypermethylation of multiple promoter CpG islands. Little is known about the clinical outcome or histologic characteristics of CIMP-positive colorectal cancers defined by recently identified CpG island methylator phenotype panels. Objective.—To investigate and compare the molecular and clinicopathologic features of CIMP-positive colorectal cancers defined by classic (p16, hMLH1, MINT1, MINT2, MINT31) and new (CACNA1G, IGF2, NEUROG1, RUNX3, SOCS1) CIMP panels. Design.—We analyzed 130 colorectal cancers for hypermethylation of both panels using methylation-specific polymerase chain reaction. Results.—With at least 2 markers methylated, both classic (39/130; 23.1%) and new (23.1%) CIMP-positive colorectal cancers were significantly associated with proximal tumor location, microsatellite instability, and BRAF mutation (all P values were less than .05). The new panel outperformed the classic panel in detecting these features. With at least 3 markers methylated, new CIMP-positive colorectal cancers (16.9%) were closely associated with proximal tumor location, low frequency of KRAS mutation, and high frequency of BRAF mutation (all P values were less than .05), whereas classic CIMP-positive colorectal cancers (18.5%) were closely associated with proximal tumor location, frequent microsatellite instability, and frequent BRAF mutation (all P values were less than .05). Analyzing a combination of CIMP and microsatellite instability status, CIMP-positive/microsatellite instability–negative colorectal cancers had the worst clinical outcomes. Conclusions.—Whereas the classic panel outperformed in predicting clinical outcome, the new panel was superior in detecting known clinicopathologic features of CIMP but inferior in prognostication power.

Abstract 2889: Distinct microbiome in colorectal cancers with the CpG Island Methylator Phenotype

2015 ◽  
Author(s):  
Ang Sun ◽  
Matteo Cesaroni ◽  
Christian Jobin ◽  
Carlos Barrero ◽  
Jaroslav Jelinek ◽  
...  
Keyword(s):  
Cpg Island ◽  
Colorectal Cancers ◽  
Cpg Island Methylator Phenotype ◽  
Methylator Phenotype

CPG island methylator phenotype and patients with multiple colorectal cancers

2000 ◽  
Vol 118 (4) ◽  
pp. A46
Author(s):  
William Mallory Grady ◽  
Sanford Markowitz ◽  
Joseph Willis
Keyword(s):  
Cpg Island ◽  
Colorectal Cancers ◽  
Cpg Island Methylator Phenotype ◽  
Methylator Phenotype
Sign in / Sign up

Export Citation Format

Share Document