scholarly journals Multiple low‐frequency and rare HLA‐B allelic variants are associated with reduced risk in 1,105 nasopharyngeal carcinoma patients in Hunan province, southern China

2020 ◽  
Vol 147 (5) ◽  
pp. 1397-1404 ◽  
Author(s):  
Wei Tian ◽  
FaMing Zhu ◽  
JinHong Cai ◽  
LiXin Li ◽  
HeKun Jin ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Southern China ◽  
Low Frequency ◽  
Hunan Province ◽  
Allelic Variants ◽  
Reduced Risk

scholarly journals Genome-Wide Analysis of 18 Epstein-Barr Viruses Isolated from Primary Nasopharyngeal Carcinoma Biopsy Specimens

2017 ◽  
Vol 91 (17) ◽  
Author(s):  
Chaofeng Tu ◽  
Zhaoyang Zeng ◽  
Peng Qi ◽  
Xiayu Li ◽  
Zhengyuan Yu ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Geographic Distribution ◽  
Epstein Barr Virus ◽  
Southern China ◽  
Hunan Province ◽  
Genomic Variations ◽  
Barr Virus ◽  
Biopsy Specimens ◽  
Genome Wide ◽  
Epstein Barr

ABSTRACT Epstein-Barr virus (EBV) is a ubiquitous gammaherpesvirus that is highly prevalent in almost all human populations and is associated with many human cancers, such as nasopharyngeal carcinoma (NPC), Hodgkin's disease, and gastric carcinoma. However, in these EBV-associated cancers, only NPC exhibits remarkable ethnic and geographic distribution. We hypothesized that EBV genomic variations might contribute to the pathogenesis of different human cancers in different geographic areas. In this study, we collected 18 NPC biopsy specimens from the Hunan Province in southern China and de novo assembled 18 NPC biopsy specimen-derived EBV (NPC-EBV) genomes, designated HN1 to HN18. This was achieved through target enrichment of EBV DNA by hybridization, followed by next-generation sequencing, to reveal sequence diversity. These EBV genomes harbored 20,570 variations totally, including 20,328 substitutions, 88 insertions, and 154 deletions, compared to the EBV reference genome. Phylogenetic analysis revealed that all NPC-EBV genomes were distinct from other EBV genomes. Furthermore, HN1 to HN18 had some nonsynonymous variations in EBV genes including genes encoding latent, early lytic, and tegument proteins, such as substitutions within transmembrane domains 1 and 3 of LMP1, FoP_duplication, and zf-AD domains of ENBA1, in addition to aberrations in noncoding regions, especially in BamHI A rightward transcript microRNAs. These variations might have potential biological significance. In conclusion, we reported a genome-wide view of sequence variation in EBV isolated from primary NPC biopsy specimens obtained from the Hunan Province. This might contribute to further understanding of how genomic variations contribute to carcinogenesis, which would impact the treatment of EBV-associated cancer. IMPORTANCE Nasopharyngeal carcinoma (NPC) is highly associated with Epstein-Barr virus (EBV) infection and exhibits remarkable ethnic and geographic distribution. Hunan Province in southern China has a high incidence rate of NPCs. Here, we report 18 novel EBV genome sequences from viruses isolated from primary NPC biopsy specimens in this region, revealing whole-genome sequence diversity.

Sequence-based typing of HLA-A gene in 930 patients with nasopharyngeal carcinoma in Hunan province, southern China

2015 ◽  
Vol 86 (1) ◽  
pp. 15-20 ◽  
Author(s):  
W. Tian ◽  
F.-M. Zhu ◽  
W.-Y. Wang ◽  
J.-H. Cai ◽  
W. Zhang ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Southern China ◽  
Hunan Province

scholarly journals Current Status and Future Perspectives about Molecular Biomarkers of Nasopharyngeal Carcinoma

Cancers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 3490
Author(s):  
Pui Yan Siak ◽  
Alan Soo-Beng Khoo ◽  
Chee Onn Leong ◽  
Boon-Peng Hoh ◽  
Shiau-Chuen Cheah
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Biomarker Discovery ◽  
Southern China ◽  
Disease Diagnosis ◽  
Late Presentation ◽  
Dietary Factors ◽  
Current Status ◽  
Complex Nature ◽  
Epstein Barr Virus Infection ◽  
Potential Biomarker

Nasopharyngeal carcinoma (NPC) is an epithelial malignancy that shows a remarkable ethnic and geographical distribution. It is one of the major public health problems in some countries, especially Southern China and Southeast Asia, but rare in most Western countries. Multifactorial interactions such as Epstein–Barr virus infection, individual’s genetic susceptibility, as well as environmental and dietary factors may facilitate the pathogenesis of this malignancy. Late presentation and the complex nature of the disease have led it to become a major cause of mortality. Therefore, an effective, sensitive, and specific molecular biomarker is urgently needed for early disease diagnosis, prognosis, and prediction of metastasis and recurrence after treatment. In this review, we discuss the recent research status of potential biomarker discovery and the problems that need to be explored further for better NPC management. By studying the aberrant pattern of these candidate biomarkers that promote NPC development and progression, we are able to understand the complexity of this malignancy better, hence positing our stands better towards strategies that may provide a way forward to the discovery of more reliable and specific biomarkers for diagnosis and targeted therapeutic development.

scholarly journals Plasma Epstein-Barr virus DNA and risk of nasopharyngeal carcinoma in a prospective seropositive population

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Wen-Jie Chen ◽  
Wen-Na Xu ◽  
Hai-Yun Wang ◽  
Xiao-Xia Chen ◽  
Xue-Qi Li ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Screening Program ◽  
Southern China ◽  
Incidence Rates ◽  
Cox Proportional Hazards ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

Abstract Objective Plasma Epstein-Barr virus (EBV) DNA is considered a biomarker for nasopharyngeal carcinoma (NPC). However, its long-term role in NPC development is unclear. Materials and methods A total of 1363 participants seropositive for EBV VCA-IgA and EBNA1-IgA in a community-based NPC screening program in southern China were tested for plasma EBV DNA levels by real-time qPCR between 2008 and 2015. New NPC cases were confirmed by active follow-up approach and linkage to local cancer registry through the end of 2016. Cox proportional hazards regression analysis was performed to calculate the hazard ratios (HRs) for NPC risk with plasma EBV DNA. Results Thirty patients were newly diagnosed during a median 7.5 years follow-up. NPC incidence increased with the plasma EBV DNA load ranging from 281.46 to 10,074.47 per 100,000 person-years in participants with undetectable and ≥ 1000 copies/ml levels; the corresponding cumulative incidence rates were 1.73 and 50%. Furthermore, plasma EBV DNA loads conferred an independent risk for NPC development after adjustment for other risk factors, with HRs of 7.63 for > 3–999 copies/ml and 39.79 for ≥1000 copies/ml. However, the HRs decreased gradually after excluding NPC cases detected in the first 2 to 3 years and became statistically nonsignificant by excluding cases detected during the first 4 years. Conclusion Elevated plasma EBV DNA can predict NPC risk over 3 years. Monitoring plasma EBV DNA can be used as a complementary approach to EBV serological antibody-based screening for NPC.

scholarly journals Transcriptional patterns, biomarkers and pathways characterizing nasopharyngeal carcinoma of Southern China

2008 ◽  
Vol 6 (1) ◽  
pp. 32 ◽  
Author(s):  
Weiyi Fang ◽  
Xin Li ◽  
Qingping Jiang ◽  
Zhen Liu ◽  
Huiling Yang ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Southern China

scholarly journals Src-1 and SP2 promote the proliferation and epithelial–mesenchymal transition of nasopharyngeal carcinoma

Open Medicine ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. 1061-1069
Author(s):  
Jingjing Zhang ◽  
Yuanyuan Yang ◽  
Hongyu Liu ◽  
Hongyi Hu
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Cell Viability ◽  
Colony Formation ◽  
Epithelial Mesenchymal Transition ◽  
Southern China ◽  
Mrna Level ◽  
Migration And Invasion ◽  
High Morbidity ◽  
Mesenchymal Transition ◽  
Steroid Receptor Coactivator

Abstract Nasopharyngeal carcinoma (NPC) is characterized by high morbidity and morality, especially in Southern China. Transcription factors intensively participate in the initiation and development of NPC. This study aimed to investigate the roles of Src-1 in NPC. mRNA level was determined by qRT-PCR. Western blot was carried out for the protein level. CCK-8 assay was performed to determine cell viability, colony formation for NPC cell proliferation, and transwell for cell migration and invasion ability. The results showed Steroid receptor coactivator 1 (Src-1) was overexpressed in SNE-2 and 6-10B. The expression of Src-1 and SP2 was in positive correlation. Overexpression of Src-1 promoted the cell viability, colony formation, and epithelial–mesenchymal transition (EMT), manifested by the increase of migration and invasion ability, while knockdown of Src-1 exerted opposite effects. Additionally, knockdown or overexpression of SP2 reversed the effects of overexpressed or downregulated Src-1, which was reversed by the depletion of SP2. Moreover, Src-1 interacted with SP2 to regulate EMT-related genes such as E-cad, N-cad, Vimentin, and ZEB1, and proliferation- and apoptosis-related genes, such as bax, cytochrome c, and cleaved caspase3 and bcl-2. Thus, blocking the interaction between Src-1 and SP2 may be a therapeutic target for inhibiting the metastasis of NPC.

Nasopharyngeal carcinoma: molecular pathogenesis and therapeutic developments

2007 ◽  
Vol 9 (12) ◽  
pp. 1-24 ◽  
Author(s):  
Qian Tao ◽  
Anthony T.C. Chan
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Cell Function ◽  
Southern China ◽  
Intensity Modulated Radiation Therapy ◽  
Tumour Suppressor Genes ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr ◽  
New Biomarkers ◽  
Multiple Tumour

Nasopharyngeal carcinoma (NPC) is a prevalent tumour in southern China and southeast Asia, particularly in the Cantonese population, where its incidence has remained high for decades. Recent studies have demonstrated that the aetiology of NPC is complex, involving multiple factors including genetic susceptibility, infection with the Epstein–Barr virus (EBV) and exposure to chemical carcinogens. During development of the disease, viral infection and multiple somatic genetic and epigenetic changes synergistically disrupt normal cell function, thus contributing to NPC pathogenesis. NPC is highly radiosensitive and chemosensitive, but treatment of patients with locoregionally advanced disease remains problematic. New biomarkers for NPC, including EBV DNA copy number or methylation of multiple tumour suppressor genes, which can be detected in serum and nasopharyngeal brushings, have been developed for the molecular diagnosis of this tumour. Meanwhile, new therapeutic strategies such as intensity-modulated radiation therapy and immuno- and epigenetic therapies might lead to more specific and effective treatments.

Rhinogobius maculagenys, A new species of freshwater goby (Teleostei: Gobiidae) from Hunan, China

Zootaxa ◽  
2018 ◽  
Vol 4476 (1) ◽  
pp. 118
Author(s):  
QIANQIAN WU ◽  
XUEJIAN DENG ◽  
YANJIE WANG ◽  
YONG LIU
Keyword(s):  
New Species ◽  
Anterior Margin ◽  
Southern China ◽  
Hunan Province ◽  
Caudal Fin ◽  
Dorsal Fin ◽  
Freshwater Goby ◽  
Single Spine ◽  
A New Species ◽  
Bright Blue

A new freshwater goby, Rhinogobius maculagenys sp. nov., was collected from Hunan Province in Southern China. This species can be distinguished from all congeners by a combination of the following features: first dorsal fin with 6 spines; second dorsal fin with a single spine and 7–9 segmented rays; anal fin with a single spine and 6–8 segmented rays; pectoral fin with 16 segmented rays; 32–34 longitudinal scales; 9–13 transverse scales; 11+16=27 vertebrae; pore ω1 missing; head and body yellowish brown; cheek and opercle yellowish brown with over 30 small orange spots, branchiostegal membrane yellow with over 10 small orange spots in males and white and spotless in females; first dorsal fin trapezoidal in males and nearly semicircular in females, with large bright blue blotch in front of second spine; spines 4 and 5 longest, rear tip extending to base of second branched ray of second dorsal fin in males when adpressed, but just reaching or not reaching anterior margin of second dorsal fin in females; caudal fin with 5–6 vertical rows of brown spots; flank with several longitudinal rows of blackish-brown spots; and belly pale white.

scholarly journals Decreased oral Epstein-Barr virus DNA loads in patients with nasopharyngeal carcinoma in Southern China: A case-control and a family-based study

2018 ◽  
Vol 7 (7) ◽  
pp. 3453-3464 ◽  
Author(s):  
Wen-Qiong Xue ◽  
Yong-Qiao He ◽  
Xiao-Yu Liao ◽  
Fang-Fang Li ◽  
Ya-Fei Xu ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Southern China ◽  
Case Control ◽  
Barr Virus ◽  
Epstein Barr ◽  
Family Based
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