Detection of aberrant methylation of HOXA9 and HIC1 through multiplex MethyLight assay in serum DNA for the early detection of epithelial ovarian cancer

2020 ◽  
Vol 147 (6) ◽  
pp. 1740-1752 ◽  
Author(s):  
Alka Singh ◽  
Sameer Gupta ◽  
Jaydeep A. Badarukhiya ◽  
Manisha Sachan
Keyword(s):  
Ovarian Cancer ◽  
Early Detection ◽  
Epithelial Ovarian Cancer ◽  
Aberrant Methylation ◽  
Serum Dna ◽  
Methylight Assay

scholarly journals Circulating Exosomal miRNAs as Biomarkers in Epithelial Ovarian Cancer

Biomedicines ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1433
Author(s):  
Meng-Shin Shiao ◽  
Jia-Ming Chang ◽  
Arb-Aroon Lertkhachonsuk ◽  
Naparat Rermluk ◽  
Natini Jinawath
Keyword(s):  
Ovarian Cancer ◽  
Early Detection ◽  
Epithelial Ovarian Cancer ◽  
Early Stage ◽  
Cell Communication ◽  
Biological Molecules ◽  
Non Invasive ◽  
Exosomal Mirnas ◽  
Endogenous Reference ◽  
Treatment Responses

Failure to detect early-stage epithelial ovarian cancer (EOC) is a major contributing factor to its low survival rate. Increasing evidence suggests that different subtypes of EOC may behave as distinct diseases due to their different cells of origins, histology and treatment responses. Therefore, the identification of EOC subtype-specific biomarkers that can early detect the disease should be clinically beneficial. Exosomes are extracellular vesicles secreted by different types of cells and carry biological molecules, which play important roles in cell-cell communication and regulation of various biological processes. Multiple studies have proposed that exosomal miRNAs present in the circulation are good biomarkers for non-invasive early detection of cancer. In this review, the potential use of exosomal miRNAs as early detection biomarkers for EOCs and their accuracy are discussed. We also review the differential expression of circulating exosomal miRNAs and cell-free miRNAs between different biofluid sources, i.e., plasma and serum, and touch on the issue of endogenous reference miRNA selection. Additionally, the current clinical trials using miRNAs for detecting EOCs are summarized. In conclusion, circulating exosomal miRNAs as the non-invasive biomarkers have a high potential for early detection of EOC and its subtypes, and are likely to be clinically important in the future.

A multiplex methylation-specific PCR assay for detection of early-stage ovarian cancer using cell-free serum DNA.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. 5535-5535
Author(s):  
Beihua Kong ◽  
Qing Zhang ◽  
Guohong Hu ◽  
Qifeng Yang ◽  
Ruifen Dong ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Early Detection ◽  
Early Stage ◽  
Methylation Status ◽  
Stage I ◽  
Methylation Specific Pcr ◽  
Specific Pcr ◽  
Preoperative Serum ◽  
Serum Dna ◽  
Free Serum

5535 Background: Epithelial ovarian cancer (EOC) remains the most lethal disease among gynecological malignancies. Prompt diagnosis is challenging because of the non-specific symptoms exhibited during the early stage of the disease. So there is an urgent need for better detection methods. Here we performed this work to build up a platform of multiplex methylation-specific PCR (MSP) assay to improve the early detection of ovarian cancer, via identifying the methylation status of cell-free serum DNA. Methods: After screening, we chose seven genes (APC, RASSF1A, CDH1, RUNX3, TFPI2, SFRP5 and OPCML) with a high frequency of methylation as candidate genes to construct the multiplex-MSP assay. When methylation of at least one of the seven genes was observed, the multiplex-MSP assay was considered positive. We performed the retrospective and screening study to verify its specificity and sensitivity in the detection of EOC. Results: The methylation status of cell-free serum DNA was examined in the preoperative serum of 202 patients, including 87 EOC cases (stage I, n=41, stage II-IV, n=46), 53 benign ovarian tumors and 62 healthy controls. As expected, multiplex MSP assay achieved a sensitivity of 85.3% and a specificity of 90.5% in stage I EOC, strikingly higher than that of single CA125, producing a sensitivity of 56.1% at 64.15% specificity [p=0.0036](Table). Conclusions: Multiplex MSP assay analyzing the methylation status of cell-free serum DNA is a suitable and reliable approach to improve the early detection of ovarian cancer, potentially benefiting a broad range of applications in clinical oncology. [Table: see text]

Abstract AP07: PROTEIN AND IMAGING MARKERS FOR EARLY DETECTION OF EPITHELIAL OVARIAN CANCER

2019 ◽  
Author(s):  
Jennifer K. Barton ◽  
Anna E. Lokshin ◽  
David A. Fishman ◽  
John F. Black
Keyword(s):  
Ovarian Cancer ◽  
Early Detection ◽  
Epithelial Ovarian Cancer ◽  
Imaging Markers
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