The fidelity of cancer cells in PDX models: Characteristics, mechanism and clinical significance

2019 ◽  
Vol 146 (8) ◽  
pp. 2078-2088 ◽  
Author(s):  
Jiahao Shi ◽  
Yongyun Li ◽  
Renbing Jia ◽  
Xianqun Fan
Keyword(s):  
Cancer Cells ◽  
Clinical Significance ◽  
Pdx Models

Tu1920 Distinct Subcellular Localization of Phosphorylated ErbB2, ErbB3 and ErbB4 in Colorectal Cancer Cells and Their Relevance to Clinical Significance

2013 ◽  
Vol 144 (5) ◽  
pp. S-881
Author(s):  
Atsushi Tatsuguchi ◽  
Keigo Mitsui ◽  
Masaoki Yonezawa ◽  
Shu Tanaka ◽  
Shunji Fujimori ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Subcellular Localization ◽  
Cancer Cells ◽  
Clinical Significance ◽  
Colorectal Cancer Cells

scholarly journals PD-L1 regulates tumorigenesis and autophagy of ovarian cancer by activating mTORC signaling

2019 ◽  
Vol 39 (12) ◽  
Author(s):  
Hongmin Gao ◽  
Juan Zhang ◽  
Xiaohong Ren
Keyword(s):  
Ovarian Cancer ◽  
Immune Response ◽  
Cancer Cells ◽  
Clinical Significance ◽  
Tumour Cell ◽  
Normal Tissue ◽  
Ovarian Tumour ◽  
Ovarian Cancer Cells ◽  
Adjacent Normal Tissue ◽  
Novel Strategy

Abstract PD-L1 is a well-known immune co-stimulatory molecule that regulates tumour cell escape from immunity by suppressing the immune response. However, the clinical significance of PD-L1 in the progression of ovarian cancer is unclear. Our study demonstrated that PD-L1 is up-regulated in ovarian tumour tissue compared with its expression level in adjacent normal tissue. Furthermore, we confirmed that PD-L1 increases the proliferation of cancer cells by activating the AKT-mTORC signalling pathway, which is also enhanced by the expression of S6K, the substrate of mTORC. In addition, PD-L1 promotes the autophagy of ovarian cancer cells by up-regulating the expression of BECN1, a crucial molecule involved in the regulation of autophagy. In conclusion, PD-L1 may provide a target for the development of a novel strategy for the treatment of ovarian cancer.

scholarly journals A Simple Three-Dimensional In Vitro Culture Mimicking the In Vivo-Like Cell Behavior of Bladder Patient-Derived Xenograft Models

Cancers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1304
Author(s):  
Robson Amaral ◽  
Maike Zimmermann ◽  
Ai-Hong Ma ◽  
Hongyong Zhang ◽  
Kamilla Swiech ◽  
...  
Keyword(s):  
Drug Resistance ◽  
In Vitro Culture ◽  
Cancer Cells ◽  
Low Cost ◽  
Maintenance Cost ◽  
Tumor Spheroids ◽  
Patient Derived Xenograft ◽  
Pdx Models

Patient-derived xenograft (PDX) models allow for personalized drug selection and the identification of drug resistance mechanisms in cancer cells. However, PDX models present technical disadvantages, such as long engraftment time, low success rate, and high maintenance cost. On the other hand, tumor spheroids are emerging as an in vitro alternative model that can maintain the phenotype of cancer cells long enough to perform all assays and predict a patient’s outcome. The present work aimed to describe a simple, reproducible, and low-cost 3D in vitro culture method to generate bladder tumor spheroids using human cells from PDX mice. Cancer cells from PDX BL0293 and BL0808 models, previously established from advanced bladder cancer, were cultured in 96-well round-bottom ultra-low attachment (ULA) plates with 5% Matrigel and generated regular and round-shaped spheroids (roundness > 0.8) with a diameter larger than 400 μm and a hypoxic core (a feature related to drug resistance in solid tumors). The responses of the tumor spheroids to the antineoplastic drugs cisplatin, gemcitabine, and their combination were similar to tumor responses in in vivo studies with PDX BL0293 and BL0808 mice. Therefore, the in vitro 3D model using PDX tumor spheroids appears as a valuable tool that may predict the outcome of in vivo drug-screening assays and represents a low-cost strategy for such purpose.

scholarly journals Data on clinical significance of GAS2 in colorectal cancer cells

Data in Brief ◽  
2016 ◽  
Vol 8 ◽  
pp. 82-86 ◽  
Author(s):  
Chun-Chao Chang ◽  
Chi-Cheng Huang ◽  
Shung-Haur Yang ◽  
Chih-Cheng Chien ◽  
Chia-Long Lee ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Clinical Significance ◽  
Colorectal Cancer Cells
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