scholarly journals Spatiotemporal homogeneity and distinctness of the T-cell receptor β-chain repertoires in Epstein-Barr virus-associated primary and metastatic nasopharyngeal carcinomas

2018 ◽  
Vol 143 (3) ◽  
pp. 610-620 ◽  
Author(s):  
Yih-Lin Chung ◽  
Mei-Ling Wu
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Epstein Barr Virus ◽  
Cell Receptor ◽  
Barr Virus ◽  
Epstein Barr ◽  
Β Chain

scholarly journals Epitope-dependent Selection of Highly Restricted or Diverse T Cell Receptor Repertoires in Response to Persistent Infection by Epstein-Barr Virus

1997 ◽  
Vol 186 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Pedro-Otavio de Campos-Lima ◽  
Victor Levitsky ◽  
Martha P. Imreh ◽  
Riccardo Gavioli ◽  
Maria G. Masucci
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Epstein Barr Virus ◽  
Cell Receptor ◽  
Nuclear Antigen ◽  
Alanine Scanning Mutagenesis ◽  
Barr Virus ◽  
High Affinity ◽  
Epstein Barr ◽  
Complementarity Determining Regions

The T cell receptor (TCR) repertoires of cytotoxic responses to the immunodominant and subdominant HLA A11–restricted epitopes in the Epstein-Barr virus (EBV) nuclear antigen-4 were investigated in four healthy virus carriers. The response to the subdominant epitope (EBNA4 399-408, designated AVF) was highly restricted with conserved Vβ usage and identical length and amino acid motifs in the third complementarity-determining regions (CDR3), while a broad repertoire using different combinations of TCR-α/β V and J segments and CDR3 regions was selected by the immunodominant epitope (EBNA4 416-424, designated IVT). Distinct patterns of interaction with the A11–peptide complex were revealed for each AVF- or IVT-specific TCR clonotype by alanine scanning mutagenesis analysis. Blocking of cytotoxic function by antibodies specific for the CD8 coreceptor indicated that, while AVF-specific TCRs are of high affinity, the oligoclonal response to the IVT epitope includes both low- and high-affinity TCRs. Thus, comparison of the memory response to two epitopes derived from the same viral antigen and presented through the same MHC class I allele suggests that immunodominance may correlate with the capacity to maintain a broad TCR repertoire.

scholarly journals Development of Epstein-Barr virus-specific memory T cell receptor clonotypes in acute infectious mononucleosis.

1996 ◽  
Vol 184 (5) ◽  
pp. 1815-1824 ◽  
Author(s):  
S L Silins ◽  
S M Cross ◽  
S L Elliott ◽  
S J Pye ◽  
S R Burrows ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Cell Receptor ◽  
Memory T Cell ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr ◽  
Acute Infectious Mononucleosis

The importance of cytotoxic T lymphocytes (CTLs) in the immunosurveillance of Epstein-Barr virus (EBV)-infected B cells is firmly established, and the viral antigens of CTL recognition in latent infection are well defined. The epitopes targeted by CTLs during primary infection have not been identified, however, and there is only limited information about T cell receptor (TCR) selection. In the present report, we have monitored the development of memory TCR-beta clonotypes selected in response to natural EBV infection in a longitudinal study of an HLA-B8+ individual with acute infectious mononucleosis (IM). By stimulating peripheral blood lymphocytes with HLA-B8+ EBV-transformed B lymphoblastoid cells, the primary virus-specific CTL response was shown to include specificities for two HLA-B8-restricted antigenic determinants, FLRGRAYGL and QAKWRLQTL, which are encoded within the latent EBV nuclear antigen EBNA-3. TCR-beta sequence analysis of CTL clones specific for each epitope showed polyclonal TCR-beta repertoire selection, with structural restrictions on recognition that indicated antigen-driven selection. Furthermore, longitudinal repertoire analysis revealed long-term preservation of a multiclonal effector response throughout convalescence, with the reemergence of distinct memory T cell clonotypes sharing similar structural restrictions. Tracking the progression of specific TCR-beta clonotypes and antigen-specific TCR-V beta family gene expression in the peripheral repertoire ex vivo using semiquantitative PCR strongly suggested that selective TCR-beta expansions were present at the clonotype level, but not at the TCR-V beta family level. Overall, in this first analysis of antigen-specific TCR development in IM, a picture of polyclonal TCR stimulation is apparent. This diversity may be especially important in the establishment of an effective CTL control during acute EBV infection and in recovery from disease.

scholarly journals Targeting Epstein-Barr virus–transformed B lymphoblastoid cells using antibodies with T-cell receptor–like specificities

Blood ◽  
2016 ◽  
Vol 128 (10) ◽  
pp. 1396-1407 ◽  
Author(s):  
Junyun Lai ◽  
Wei Jian Tan ◽  
Chien Tei Too ◽  
Joanna Ai Ling Choo ◽  
Lan Hiong Wong ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
T Cell ◽  
T Cell Receptor ◽  
Epstein Barr Virus ◽  
Cell Receptor ◽  
Barr Virus ◽  
Key Points ◽  
Tumor Load ◽  
Epstein Barr

Key Points Anti-EBV TCR-like monoclonal antibodies reduce BLCLs tumor load in vivo. Anti-EBV TCR-like monoclonal antibodies mediate phagocytosis of BLCLs by macrophages.

T-cell receptor gene rearrangement in Epstein–Barr virus infectious mononucleosis

2011 ◽  
Vol 29 (3) ◽  
pp. 2300-2302 ◽  
Author(s):  
L. Marbello ◽  
M. Riva ◽  
S. Veronese ◽  
A. M. Nosari ◽  
E. Ravano ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Epstein Barr Virus ◽  
Gene Rearrangement ◽  
Receptor Gene ◽  
Cell Receptor ◽  
Barr Virus ◽  
T Cell Receptor Gene ◽  
Epstein Barr ◽  
Cell Receptor Gene

T-cell Receptor (TCR) Phenotype of Nodal Epstein-Barr Virus (EBV)-positive Cytotoxic T-cell Lymphoma (CTL)

2015 ◽  
Vol 39 (4) ◽  
pp. 462-471 ◽  
Author(s):  
Seiichi Kato ◽  
Naoko Asano ◽  
Tomoko Miyata-Takata ◽  
Katsuyoshi Takata ◽  
Ahmed Ali Elsayed ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Epstein Barr Virus ◽  
Cell Lymphoma ◽  
Cell Receptor ◽  
T Cell Lymphoma ◽  
Cytotoxic T Cell ◽  
Barr Virus ◽  
Epstein Barr
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