scholarly journals Long-distance interaction of the integrated HPV fragment with MYC gene and 8q24.22 region upregulating the allele-specific MYC expression in HeLa cells

2017 ◽  
Vol 141 (3) ◽  
pp. 540-548 ◽  
Author(s):  
Congle Shen ◽  
Yongzhen Liu ◽  
Shu Shi ◽  
Ruiyang Zhang ◽  
Ting Zhang ◽  
...  
Keyword(s):  
Hela Cells ◽  
Long Distance ◽  
Myc Gene ◽  
Allele Specific ◽  
Distance Interaction

POTTERY TRADE AND THE FORMATION OF EARLY AND MIDDLE FORMATIVE STYLE HORIZONS AS SEEN FROM CENTRAL MEXICO

2019 ◽  
Vol 30 (2) ◽  
pp. 311-337 ◽  
Author(s):  
Wesley D. Stoner ◽  
Deborah L. Nichols
Keyword(s):  
Gulf Coast ◽  
Petrographic Analysis ◽  
Long Distance ◽  
University Of Missouri ◽  
Late Formative ◽  
Middle Formative ◽  
The University ◽  
The Relationship ◽  
Distance Interaction ◽  
Early Formative

AbstractWe explore the relationship between long-distance pottery trade and the formation of Early and Middle Formative style horizons in Mesoamerica. A sample of 1,154 ceramics mostly from Early and Middle Formative contexts in the central Mexican highlands was irradiated at the University of Missouri Research Reactor with a subsample (n = 313) for petrographic analysis. We conclude that: (1) most sites and regions display more than one process for making pottery; (2) there is a small amount of intraregional exchange among central Mexican sites, with the southeastern Basin of Mexico making the largest portion of pottery intended for trade within the region; and (3) interregional imports found at several sites likely come from the metamorphic region of southwestern Puebla with smaller numbers imported from the southern Gulf Coast, Morelos, and possibly Oaxaca. The trend over time from Early Formative to the end of the Middle Formative is one of decreasing intensity of long-distance interaction and decreasing geographic range of trade. These two trends contribute to the regional divergence of ceramic styles that peaks by the Late Formative in Mesoamerica.

scholarly journals Allele-specific expression identified rs2509956 as a novel long-distance cis-regulatory SNP for SCGB1A1, an important gene for multiple pulmonary diseases

2019 ◽  
Vol 317 (4) ◽  
pp. L456-L463
Author(s):  
Xiu-Xiong Li ◽  
Tao Peng ◽  
Jing Gao ◽  
Jia-Gang Feng ◽  
Dan-Dan Wu ◽  
...  
Keyword(s):  
Regulatory Elements ◽  
Pulmonary Diseases ◽  
Chronic Obstructive ◽  
Long Distance ◽  
Specific Expression ◽  
Allele Specific Expression ◽  
Chromosome Conformation ◽  
Functional Region ◽  
Gene Assay ◽  
Allele Specific

SCGB1A1 (secretoglobin family 1A member 1) is an important protein for multiple pulmonary diseases, especially asthma, chronic obstructive pulmonary disease, and lung cancer. One single-nucleotide polymorphism (SNP) at 5′-untranslated region of SCGB1A1, rs3741240, has been suggested to be associated with reduced protein expression and further asthma susceptibility. However, it was still unclear whether there were other cis-regulatory elements for SCGB1A1 that might further contribute to pulmonary diseases. Allele-specific expression (ASE) is a novel approach to identify the functional region in human genome. In the present study, we measured ASE on rs3741240 in lung tissues and observed a consistent excess of G allele over A ( P < 10−6), which indicated that this SNP or the one(s) in linkage disequilibrium (LD) could regulate SCGB1A1 expression. By analyzing 1000 Genomes Project data for Chinese, one SNP locating ~10.2 kb away and downstream of SCGB1A1, rs2509956, was identified to be in strong LD with rs3741240. Reporter gene assay confirmed that both SNPs could regulate gene expression in the lung cell. By chromosome conformation capture, it was verified that the region surrounding rs2509956 could interact with SCGB1A1 promoter region and act as an enhancer. Through chromatin immunoprecipitation and overexpression assay, the related transcription factor RELA (RELA proto-oncogene, NF-kB subunit) was recognized to bind the region spanning rs2509956. Our work identified a novel long-distance cis-regulatory SNP for SCGB1A1, which might contribute to multiple pulmonary diseases.

scholarly journals Opposite replication polarity of the germ line c-myc gene in HeLa cells compared with that of two Burkitt lymphoma cell lines.

1989 ◽  
Vol 9 (2) ◽  
pp. 586-593 ◽  
Author(s):  
M Leffak ◽  
C D James
Keyword(s):  
Cell Lines ◽  
Hela Cells ◽  
Burkitt Lymphoma ◽  
Germ Line ◽  
Lymphoma Cell ◽  
Cell Type Specificity ◽  
Intact Cells ◽  
Burkitt Lymphoma Cell ◽  
Myc Gene

To study the cell type specificity of the direction of replication of the human c-myc genes and the relationship of replication polarity to transcriptional activity, we analyzed the directions of replication of the c-myc genes in two Burkitt lymphoma cell lines, CA46 and ST486, and in HeLa cells. On the basis of in vitro runoff replication of forks initiated in intact cells, we found that transcribed c-myc genes in the germ line configuration in HeLa cells were replicated in the direction of transcription from origins in the 5'-flanking DNA, while the repressed, unrearranged c-myc genes of CA46 and ST486 cells were replicated in the antitranscriptional direction. In contrast, the transcribed c-myc genes of CA46 cells were replicated in the transcriptional direction, while the translocated, amplified c-myc genes of ST486 cells showed no preferred polarity of replication. The data also provided evidence for the existence of an endogenous barrier to DNA polymerases in the flanking DNA immediately 5' to the HeLa c-myc genes.

scholarly journals An intronic structure enabled by a long-distance interaction serves as a novel target for splicing correction in spinal muscular atrophy

2013 ◽  
Vol 41 (17) ◽  
pp. 8144-8165 ◽  
Author(s):  
Natalia N. Singh ◽  
Mariah N. Lawler ◽  
Eric W. Ottesen ◽  
Daya Upreti ◽  
Jennifer R. Kaczynski ◽  
...  
Keyword(s):  
Spinal Muscular Atrophy ◽  
Muscular Atrophy ◽  
Long Distance ◽  
Novel Target ◽  
Distance Interaction

An initiation zone of chromosomal DNA replication located upstream of the c-myc gene in proliferating HeLa cells

1990 ◽  
Vol 10 (9) ◽  
pp. 4899-4904
Author(s):  
L Vassilev ◽  
E M Johnson
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Replication ◽  
Hela Cells ◽  
Polymerase Chain Reaction Amplification ◽  
Chromosomal Dna ◽  
Chain Reaction ◽  
Initiation Zone ◽  
Myc Gene ◽  
Reaction Amplification ◽  
Polymerase Chain

Studies on origins of DNA replication in mammalian cells have long been hampered by a lack of methods sensitive enough for the localization of such origins in chromosomal DNA. We have employed a new method for mapping origins, based on polymerase chain reaction amplification of nascent strand segments, to examine replication initiated in vivo near the c-myc gene in human cells. Nascent DNA, pulse-labeled in unsynchronized HeLa cells, was size fractionated and purified by immunoprecipitation with anti-bromodeoxyuridine antibodies. Lengths of the nascent strands that allow polymerase chain reaction amplification were determined by hybridization to probes homologous to amplified segments and used to calculate the position of the origin. We found that DNA replication through the c-myc gene initiates in a zone centered approximately 1.5 kilobases upstream of exon I. Replication proceeds bidirectionally from the origin, as indicated by comparison of hybridization patterns for three amplified segments. The initiation zone includes segments of the c-myc locus previously reported to drive autonomous replication of plasmids in human cells.

SOLIDS ASPECTS OF INTERACTION OF MATERIAL BODIES IN THE PROBLEMS OF GENERAL ASTRONOMY

2016 ◽  
Vol 9 (1) ◽  
pp. 14-20
Author(s):  
Котов ◽  
P. Kotov
Keyword(s):  
Theoretical Model ◽  
Homogeneous Space ◽  
Dynamic State ◽  
Long Distance ◽  
Material Bodies ◽  
Distance Interaction

The theoretical model of long-distance interaction of material bodies in homogeneous space of measurable extent is considered and constructive aspects of stability of dynamic state with calculation for summing actions are offered.

The children of Kaminaljuyu: Isotopic insight into diet and long distance interaction in Mesoamerica

2010 ◽  
Vol 29 (2) ◽  
pp. 155-178 ◽  
Author(s):  
Lori E. Wright ◽  
Juan Antonio Valdés ◽  
James H. Burton ◽  
T. Douglas Price ◽  
Henry P. Schwarcz
Keyword(s):  
Long Distance ◽  
Distance Interaction ◽  
Insight Into
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