scholarly journals Deletion of macrophage migration inhibitory factor inhibits murine oral carcinogenesis: Potential role for chronic pro-inflammatory immune mediators

2016 ◽  
Vol 139 (6) ◽  
pp. 1379-1390 ◽  
Author(s):  
Steve Oghumu ◽  
Thomas J. Knobloch ◽  
Cesar Terrazas ◽  
Sanjay Varikuti ◽  
Jennifer Ahn-Jarvis ◽  
...  
Keyword(s):  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Potential Role ◽  
Inhibitory Factor ◽  
Macrophage Migration ◽  
Oral Carcinogenesis ◽  
Immune Mediators

scholarly journals Potential role of macrophage migration inhibitory factor in the pathogenesis of Marek’s disease

2020 ◽  
Vol 64 (1) ◽  
pp. 33-38
Author(s):  
Zhongjun Fan ◽  
Huanli Wang ◽  
Jiahao Pan ◽  
Shupei Yu ◽  
Wenlong Xia
Keyword(s):  
T Cell ◽  
Virus Infection ◽  
Migration Inhibitory Factor ◽  
Post Infection ◽  
Macrophage Migration Inhibitory Factor ◽  
Potential Role ◽  
Marek's Disease ◽  
Inhibitory Factor ◽  
Macrophage Migration

AbstractIntroductionMarek’s disease virus (MDV) can cause malignant T-cell lymphomas and immunosuppression in chickens. Macrophage migration inhibitory factor (MIF) not only plays a critical role in inhibiting T-cell responses, but also contributes to multiple aspects of tumour progression. The aim of this study was to reveal the potential role of MIF in the pathogenesis of MDV infection.Material and MethodsMIF gene expression levels were measured by using real-time PCR. Expression was assayed at different times in chicken embryo fibroblast (CEF) cells and tissue samples of SPF chickens infected with different MDV strains and fold change was calculated by the 2–△△CT method.ResultsThe expression of MIF was significantly downregulated (p < 0.05 and FC > 2) in CEF cells infected with the very virulent MDV RB1B strain at 48 h post infection (hpi) and in the skin and spleen at 14 days post infection (dpi). The reduction of MIF expression was also found in CEF cells infected by reticuloendotheliosis virus (REV), avian leukosis virus subgroup J (ALV-J), and MDV vaccine strain CVI988 or in HD11 cells stimulated with TLR2, 3, 4, and 7 ligands. Interestingly, MIF expression decreased continuously from 7 to 28 dpi in the thymus after RB1B virus infection while it increased after CVI988 virus infection. Upregulated expression of MIF was found in CEF infected with RB1B at 96 hpi and in the spleen and skin at 21 and 28 dpi.ConclusionThe present study revealed the different expression pattern of MIF in response to MDV infection and indicated that MIF level may be associated with MDV pathogenesis.

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