scholarly journals Effects of the exercise-inducible myokine irisin on malignant and non-malignant breast epithelial cell behaviorin vitro

2014 ◽  
Vol 136 (4) ◽  
pp. E197-E202 ◽  
Author(s):  
Nicholas P. Gannon ◽  
Roger A. Vaughan ◽  
Randi Garcia-Smith ◽  
Marco Bisoffi ◽  
Kristina A. Trujillo
Keyword(s):  
Epithelial Cell ◽  
Breast Epithelial Cell ◽  
Malignant Breast ◽  
Breast Epithelial

scholarly journals β-alanine suppresses malignant breast epithelial cell aggressiveness through alterations in metabolism and cellular acidity in vitro

2014 ◽  
Vol 13 (1) ◽  
pp. 14 ◽  
Author(s):  
Roger A Vaughan ◽  
Nicholas P Gannon ◽  
Randi Garcia-Smith ◽  
Yamhilette Licon-Munoz ◽  
Miguel A Barberena ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Breast Epithelial Cell ◽  
Malignant Breast ◽  
Breast Epithelial

scholarly journals Expression of Tropomyosin 1 Gene Isoforms in Human Breast Cancer Cell Lines

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Syamalima Dube ◽  
Santhi Yalamanchili ◽  
Joseph Lachant ◽  
Lynn Abbott ◽  
Patricia Benz ◽  
...  
Keyword(s):  
Cell Lines ◽  
Malignant Cell ◽  
Normal Breast ◽  
Stress Fiber ◽  
Fiber Formation ◽  
Breast Epithelial Cell ◽  
Breast Cell Lines ◽  
Stress Fiber Formation ◽  
Malignant Breast ◽  
Breast Epithelial

Nine malignant breast epithelial cell lines and 3 normal breast cell lines were examined for stress fiber formation and expression of TPM1 isoform-specific RNAs and proteins. Stress fiber formation was strong (++++) in the normal cell lines and varied among the malignant cell lines (negative to +++). Although TPM1γand TPM1δwere the dominant transcripts ofTPM1, there was no clear evidence for TPM1δprotein expression. Four novel human TPM1 gene RNA isoforms were discovered (λ,μ,ν, andξ), which were not identified in adult and fetal human cardiac tissues. TPM1λwas the most frequent isoform expressed in the malignant breast cell lines, and it was absent in normal breast epithelial cell lines. By western blotting, we were unable to distinguish between TPM1γ,λ, andνprotein expression, which were the only TPM1 gene protein isoforms potentially expressed. Some malignant cell lines demonstrated increased or decreased expression of these isoforms relative to the normal breast cell lines. Stress fiber formation did not correlate with TPM1γRNA expression but significantly and inversely correlated with TPM1δand TPM1λexpression, respectively. The exact differences in expression of these novel isoforms and their functional properties in breast epithelial cells will require further study.

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