Biochemical characterization of the monoclonal antibody-defined ovarian carcinoma-associated antigen SGA

1986 ◽  
Vol 37 (5) ◽  
pp. 705-712 ◽  
Author(s):  
Theonne A. de Kretser ◽  
Heather J. Thorne ◽  
Diana Picone ◽  
David G. Jose
Keyword(s):  
Monoclonal Antibody ◽  
Ovarian Carcinoma ◽  
Biochemical Characterization

Generation and characterization of human monoclonal antibody HMD4 against ovarian carcinoma and the study of radioimmunoimaging in nude mice

1990 ◽  
Vol 1 (2) ◽  
pp. 104-110 ◽  
Author(s):  
Henian Qian ◽  
Heng Cui ◽  
Jie Feng ◽  
Tianyuan Fu ◽  
Ping Wei ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Ovarian Carcinoma ◽  
Nude Mice ◽  
Human Monoclonal Antibody

In Silico Analysis and Immunohistochemical Characterization of NaPi2b Protein Expression in Ovarian Carcinoma With Monoclonal Antibody Mx35

2012 ◽  
Vol 20 (2) ◽  
pp. 165-172 ◽  
Author(s):  
Ibere Cauduro Soares ◽  
Kleber Simões ◽  
Jorge Estefano Santana de Souza ◽  
Oswaldo Keith Okamoto ◽  
Alda Wakamatsu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Protein Expression ◽  
Ovarian Carcinoma ◽  
In Silico ◽  
In Silico Analysis ◽  
Silico Analysis

Biochemical Characterization of a Monoclonal Antibody to the H Type-2 Antigen: Comparison to Other ABH Antibodies

Hybridoma ◽  
1986 ◽  
Vol 5 (2) ◽  
pp. 117-127 ◽  
Author(s):  
BARNETT B. ROSENBLUM ◽  
W. JOHN JUDD ◽  
THOMAS E. CAREY
Keyword(s):  
Monoclonal Antibody ◽  
Biochemical Characterization

scholarly journals Biochemical Characterization of Human Anti-Hepatitis B Monoclonal Antibody Produced in the Microalgae Phaeodactylum tricornutum

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0139282 ◽  
Author(s):  
Gaëtan Vanier ◽  
Franziska Hempel ◽  
Philippe Chan ◽  
Michael Rodamer ◽  
David Vaudry ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Hepatitis B ◽  
Phaeodactylum Tricornutum ◽  
Biochemical Characterization

scholarly journals Acetylated alpha-tubulin in Physarum: immunological characterization of the isotype and its usage in particular microtubular organelles.

1987 ◽  
Vol 104 (1) ◽  
pp. 41-49 ◽  
Author(s):  
R Sasse ◽  
M C Glyn ◽  
C R Birkett ◽  
K Gull
Keyword(s):  
Monoclonal Antibody ◽  
Mitotic Spindle ◽  
Biochemical Characterization ◽  
Alpha Tubulin ◽  
Acetylated Tubulin ◽  
Tubulin Isotype ◽  
Cytoplasmic Microtubules ◽  
Spindle Microtubules ◽  
Microtubular Organelles

We have used monoclonal antibodies specific for acetylated and unacetylated alpha-tubulin to characterize the acetylated alpha-tubulin isotype of Physarum polycephalum, its expression in the life cycle, and its localization in particular microtubular organelles. We have used the monoclonal antibody 6-11B-1 (Piperno, G., and M. T. Fuller, 1985, J. Cell Biol., 101:2085-2094) as the probe for acetylated alpha-tubulin and have provided a biochemical characterization of the monoclonal antibody KMP-1 as a probe for unacetylated tubulin in Physarum. Concomitant use of these two probes has allowed us to characterize the acetylated alpha-tubulin of Physarum as the alpha 3 isotype. We have detected this acetylated alpha 3 tubulin isotype in both the flagellate and in the myxameba, but not in the plasmodium. In the flagellate, acetylated tubulin is present in both the flagellar axonemes and in an extensive array of cytoplasmic microtubules. The extensive arrangement of acetylated cytoplasmic microtubules and the flagellar axonemes are elaborated during the myxameba-flagellate transformation. In the myxameba, acetylated tubulin is not present in the cytoplasmic microtubules nor in the mitotic spindle microtubules, but is associated with the two centrioles of this cell. These findings, taken together with the apparent absence of acetylated alpha-tubulin in the ephemeral microtubules of the plasmodium suggest a natural correspondence between the presence of acetylated alpha-tubulin and microtubule organelles that are intrinsically stable or cross-linked.

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