Modulation of the expression of alkaline phosphatase genes in a human malignant cell line passaged through nude mice

1981 ◽  
Vol 28 (3) ◽  
pp. 257-264 ◽  
Author(s):  
Frances J. Benham ◽  
Gloria Balaban ◽  
Denise Boccelli ◽  
Harry Harris
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Line ◽  
Nude Mice ◽  
Malignant Cell ◽  
Malignant Cell Line

scholarly journals Cytotoxic Properties of Lyophilized Beers in a Malignant Cell Line

2014 ◽  
Vol 05 (01) ◽  
pp. 45-51 ◽  
Author(s):  
Carmela Spagnuolo ◽  
Idolo Tedesco ◽  
Maria Grazia Volpe ◽  
Stefania Bilotto ◽  
Maria Russo ◽  
...  
Keyword(s):  
Cell Line ◽  
Malignant Cell ◽  
Malignant Cell Line

Lyophilized whole human melanoma cells enhanced suppressive action of PBMC toward survival of the corresponding malignant cell line in vitro

1999 ◽  
Vol 35 ◽  
pp. S358-S359
Author(s):  
Z.D. Juranic ◽  
T. Stanojkovic ◽  
N. Stanojevic-Bakic ◽  
D. Milosevic ◽  
S. Radulovic ◽  
...  
Keyword(s):  
Cell Line ◽  
Malignant Cell ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Malignant Cell Line ◽  
Human Melanoma Cells ◽  
Suppressive Action

scholarly journals Evidence for a pre-malignant cell line in a skin biopsy from a patient with Nijmegen breakage syndrome

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Raneem Habib ◽  
Heidemarie Neitzel ◽  
Aurelie Ernst ◽  
John K. L. Wong ◽  
Bozenna Goryluk-Kozakiewicz ◽  
...  
Keyword(s):  
Skin Biopsy ◽  
Cell Line ◽  
Nijmegen Breakage Syndrome ◽  
Malignant Cell ◽  
Malignant Cell Line

scholarly journals Correction to: Separating mouse malignant cell line (EL4) from neonate spermatogonial stem cells utilizing microfluidic device in vitro

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Behnaz Ashtari ◽  
Azar Shams ◽  
Narges Esmaeilzadeh ◽  
Sara Tanbakooei ◽  
Morteza Koruji ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Line ◽  
Microfluidic Device ◽  
Malignant Cell ◽  
Spermatogonial Stem Cells ◽  
Malignant Cell Line

An amendment to this paper has been published and can be accessed via the original article.

scholarly journals An image-based flow cytometric approach to the assessment of the nucleus-to-cytoplasm ratio

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0253439
Author(s):  
Joseph A. Sebastian ◽  
Michael J. Moore ◽  
Elizabeth S. L. Berndl ◽  
Michael C. Kolios
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
High Throughput ◽  
Malignant Cell ◽  
Cell Diameter ◽  
Average Cell ◽  
Malignant Cell Line ◽  
Assessment Techniques ◽  
High Throughput Detection ◽  
Ht 29

The nucleus-to-cytoplasm ratio (N:C) can be used as one metric in histology for grading certain types of tumor malignancy. Current N:C assessment techniques are time-consuming and low throughput. Thus, in high-throughput clinical contexts, there is a need for a technique that can assess cell malignancy rapidly. In this study, we assess the N:C ratio of four different malignant cell lines (OCI-AML-5—blood cancer, CAKI-2—kidney cancer, HT-29—colon cancer, SK-BR-3—breast cancer) and a non-malignant cell line (MCF-10A –breast epithelium) using an imaging flow cytometer (IFC). Cells were stained with the DRAQ-5 nuclear dye to stain the cell nucleus. An Amnis ImageStreamX® IFC acquired brightfield/fluorescence images of cells and their nuclei, respectively. Masking and gating techniques were used to obtain the cell and nucleus diameters for 5284 OCI-AML-5 cells, 1096 CAKI-2 cells, 6302 HT-29 cells, 3159 SK-BR-3 cells, and 1109 MCF-10A cells. The N:C ratio was calculated as the ratio of the nucleus diameter to the total cell diameter. The average cell and nucleus diameters from IFC were 12.3 ± 1.2 μm and 9.0 ± 1.1 μm for OCI-AML5 cells, 24.5 ± 2.6 μm and 15.6 ± 2.1 μm for CAKI-2 cells, 16.2 ± 1.8 μm and 11.2 ± 1.3 μm for HT-29 cells, 18.0 ± 3.7 μm and 12.5 ± 2.1 μm for SK-BR-3 cells, and 19.4 ± 2.2 μm and 10.1 ± 1.8 μm for MCF-10A cells. Here we show a general N:C ratio of ~0.6–0.7 across varying malignant cell lines and a N:C ratio of ~0.5 for a non-malignant cell line. This study demonstrates the use of IFC to assess the N:C ratio of cancerous and non-cancerous cells, and the promise of its use in clinically relevant high-throughput detection scenarios to supplement current workflows used for cancer cell grading.

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