Mouse mammary tumor virus (MMTV) antigen(s) are present on B lymphocytes of BALB/c mice

1979 ◽  
Vol 24 (6) ◽  
pp. 813-818 ◽  
Author(s):  
Vijaya Charyulu ◽  
M. Michael Sigel ◽  
Donald L. Durden ◽  
Diana M. Lopez
Keyword(s):  
B Lymphocytes ◽  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Mammary Tumor Virus ◽  
Mouse Mammary Tumor ◽  
Tumor Virus

scholarly journals Preactivation of B Lymphocytes Does Not Enhance Mouse Mammary Tumor Virus Infection

1998 ◽  
Vol 72 (9) ◽  
pp. 7688-7691 ◽  
Author(s):  
Daniela Finke ◽  
Laure Mortezavi ◽  
Hans Acha-Orbea
Keyword(s):  
B Cells ◽  
B Lymphocytes ◽  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Mammary Tumor Virus ◽  
Cell Responses ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
Lower Infection

ABSTRACT We investigated whether mouse mammary tumor virus (MMTV) favors preactivated or naive B cells as targets for efficient infection. We have demonstrated previously that MMTV activates B cells upon infection. Here, we show that polyclonal activation of B cells leads instead to lower infection levels and attenuated superantigen-specific T-cell responses in vivo. This indicates that naive small resting B cells are the major targets of MMTV infection and that the activation induced by MMTV is sufficient to allow efficient infection.

scholarly journals Preferential Binding of Mouse Mammary Tumor Virus to B Lymphocytes

1999 ◽  
Vol 73 (9) ◽  
pp. 7899-7902 ◽  
Author(s):  
Frédéric Baribaud ◽  
Annelyse Vessaz Shaw ◽  
Leo Scarpellino ◽  
Heidi Diggelmann ◽  
Hans Acha-Orbea
Keyword(s):  
B Lymphocytes ◽  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Neutralizing Antibodies ◽  
Mammary Tumor Virus ◽  
Viral Receptor ◽  
Mouse Mammary Tumor ◽  
Preferential Binding ◽  
Tumor Virus

ABSTRACT Mouse mammary tumor virus (MMTV) has been shown to preferentially infect B lymphocytes in vivo. We have used recombinant envelope-coated fluospheres and highly purified MMTV particles to study the distribution of the viral receptors on fresh mouse lymphocytes. A preferential dose-dependent binding to B lymphocytes was observed which could be competed with neutralizing antibodies. In contrast, T-lymphocyte binding remained at background levels. These results strongly suggest a higher density of viral receptor molecules on B lymphocytes than on T lymphocytes and correlate with the preferential initial infection of B lymphocytes observed in vivo.

Electron Microscopy of the Nucleic Acid of Mouse Mammary Tumor Virus

1968 ◽  
Vol 26 ◽  
pp. 22-23
Author(s):  
N. H. Sarkar ◽  
Dan H. Moore
Keyword(s):  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Ammonium Acetate ◽  
Dry Weight ◽  
Mouse Tumor ◽  
Mammary Tumor Virus ◽  
Rna Molecules ◽  
Mouse Mammary Tumor ◽  
Tumor Virus ◽  
The Subject

Mouse mammary tumor virus (MTV) is believed to contain about 0.8% single stranded ribonucleic acid (RNA). This value of RNA content was estimated on a dry weight basis. The subject of this report is an attempt to visualize the RNA molecules of MTV particles.MTV particles were isolated from RIII mouse (tumor incidence approximately 80%) milk according to the method described by Lyons and Moore. Purified virions from 5 ml of milk were finally suspended in 0.2 ml of PBS, pH 7.4 and was mixed with an equal volume of pronase (5 mg/ml). This mixture was incubated at 37°C for an hour. RNA was extracted three times using freshly prepared cold phenol. It was then treated three times with cold ethyl ether to remove any trace of phenol. The RNA thus extracted was divided into two parts. One part was diluted four fold with 8M urea to avoid aggregation of the molecules. The other part was left untreated. Both samples were then mixed with an equal volume of 1M ammonium acetate, adjusted to pH 8.0 with NH3 containing chymotrypsin at a concentration of 0.01%.

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