DNA repair synthesis of cultured human cells as a rapid bioassay for chemical carcinogens

1975 ◽  
Vol 16 (2) ◽  
pp. 284-291 ◽  
Author(s):  
R. H. C. San ◽  
H. F. Stich
Keyword(s):  
Dna Repair ◽  
Human Cells ◽  
Chemical Carcinogens ◽  
Repair Synthesis ◽  
Rapid Bioassay ◽  
Cultured Human Cells ◽  
Dna Repair Synthesis

INVITATION PAPER: THE SEARCH FOR RELEVANT SHORT TERM BIOASSAYS FOR CHEMICAL CARCINOGENS: THE TRIBULATION OF A MODERN SISYPHUS

1975 ◽  
Vol 17 (4) ◽  
pp. 471-492 ◽  
Author(s):  
H. F. Stich ◽  
P. Lam ◽  
L. W. Lo ◽  
D. J. Koropatnick ◽  
R. H. C. San
Keyword(s):  
Dna Repair ◽  
Dna Fragmentation ◽  
Mutagenic Activity ◽  
Test System ◽  
Human Cells ◽  
Human Populations ◽  
Chemical Carcinogens ◽  
Repair Synthesis ◽  
Cultured Human Cells ◽  
Dna Repair Synthesis

Based on a good correlation between carcinogenicity and mutagenic activity several rapid microbial bioassays for chemical carcinogens have been recently developed. We would like to suggest, that these microbial tests should be followed by bioassays using cultured human cells of the "average" man, and of persons with elevated cancer risk or increased susceptibility to carcinogenic agents. The main objective of using DNA repair (unscheduled uptake of3HTdR) and DNA fragmentation (shift in sedimentation profiles) of cultured human cells was to design a test system that can simulate conditions found in man and thus provide information relevant to the human population. A trial on 98 different carcinogens, precarcinogens and noncarcinogens showed the suitability of DNA repair synthesis as a rapid, economic and relevant assay for detection of chemical carcinogens. To check the adaptibility of DNA repair synthesis of human cells as a bioassay for chemical carcinogens we examined carcinogenic nitrosation products which are formed from the interaction of nitrite and nitrosatable compounds, carcinogenic or mutagenic photosensitizing chemicals, and the effect of complex interactions. Organotropic carcinogens can be detected by measuring DNA fragmentation and DNA repair in various target organs following the in vivo application of chemical carcinogens. The pros and cons of several bioassays and their usefulness in judging a carcinogenic or mutagenic hazard to human populations is discussed.

In situ enzymology of DNA replication and ultraviolet-induced DNA repair synthesis in permeable human cells

Biochemistry ◽  
1988 ◽  
Vol 27 (19) ◽  
pp. 7247-7254 ◽  
Author(s):  
Steven L. Dresler ◽  
Mark G. Frattini ◽  
Rona M. Robinson-Hill
Keyword(s):  
Dna Repair ◽  
Dna Replication ◽  
Human Cells ◽  
Repair Synthesis ◽  
Dna Repair Synthesis

Visualization of Focal Nuclear Sites of DNA Repair Synthesis Induced by Bleomycin in Human Cells

2001 ◽  
Vol 156 (4) ◽  
pp. 347-354 ◽  
Author(s):  
N. V. Tomilin ◽  
L. V. Solovjeva ◽  
M. P. Svetlova ◽  
N. M. Pleskach ◽  
I. A. Zalenskaya ◽  
...  
Keyword(s):  
Dna Repair ◽  
Human Cells ◽  
Repair Synthesis ◽  
Dna Repair Synthesis
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