Segregation of the EBV-determined nuclear antigen (EBNA) in somatic cell hybrids derived from the fusion of a mouse fibroblast and a human burkitt lymphoma line

1974 ◽  
Vol 14 (1) ◽  
pp. 54-64 ◽  
Author(s):  
George Klein ◽  
Francis Wiener ◽  
Lore Zech ◽  
Harald Zur Hausen ◽  
Beverley Reedman
Keyword(s):  
Somatic Cell ◽  
Burkitt Lymphoma ◽  
Mouse Fibroblast ◽  
Nuclear Antigen ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Lymphoma Line

Truncated Mu Chain in a Burkitt Lymphoma Line (P3HR-1) and its Fate in Various Hemapoietic Somatic Cell Hybrids

Immunobiology ◽  
1982 ◽  
Vol 162 (2) ◽  
pp. 199-209 ◽  
Author(s):  
G. Spira ◽  
N. Koide ◽  
P. Åman ◽  
Rosalie Ber ◽  
G. Klein
Keyword(s):  
Somatic Cell ◽  
Burkitt Lymphoma ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Lymphoma Line

Herpesvirus-associated nuclear antigen(s) in cells biochemically transformed by fragments of herpesvirus DNA and in somatic cell hybrids

Virology ◽  
1980 ◽  
Vol 105 (1) ◽  
pp. 103-122 ◽  
Author(s):  
Saul Kit ◽  
Haruki Otsuka ◽  
Hamida Qavi ◽  
David Trkula ◽  
Del Rose Dubbs
Keyword(s):  
Somatic Cell ◽  
Nuclear Antigen ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
In Cells

scholarly journals ACQUISITION OF CHICK CYTOSOL THYMIDINE KINASE ACTIVITY BY THYMIDINE KINASE-DEFICIENT MOUSE FIBROBLAST CELLS AFTER FUSION WITH CHICK ERYTHROCYTES

1974 ◽  
Vol 63 (2) ◽  
pp. 505-514 ◽  
Author(s):  
Saul Kit ◽  
Wai-Choi Leung ◽  
George Jorgensen ◽  
David Trkula ◽  
Del Rose Dubbs
Keyword(s):  
Somatic Cell ◽  
Thymidine Kinase ◽  
Kinase Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
Deficient Mouse ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids ◽  
Clonal Lines

Chick-mouse heterokaryons were obtained by UV-Sendai virus-induced fusion of chick erythrocytes with thymidine (dT) kinase-deficient mouse fibroblast [LM(TK-)] cells. Autoradiographic studies demonstrated that 1 day after fusion, [3H]dT was incorporated into both red blood cell and LM(TK-) nuclei of 23% of the heterokaryons. Self-fused LM(TK-) cells failed to incorporate [3H]dT into nuclear DNA. 15 clonal lines of chick-mouse somatic cell hybrids [LM(TK-)/CRB] were isolated from the heterokaryons by cultivating them in selective hypoxanthine-aminopterin-thymidine-glycine medium. LM(TK-) and chick erythrocytes exhibited little, if any, cytosol dT kinase activity. In contrast, all 15 LM(TK-)/CRB lines contained levels of cytosol dT kinase activity comparable to that found in chick embryo cells. Disk polyacrylamide gel electrophoresis and isoelectric focusing analyses demonstrated that the LM(TK-)/CRB cells contained chick cytosol, but not mouse cytosol dT kinase. The LM(TK-)/CRB cells also contained mouse mitochondrial, but not chick mitochondrial dT kinase. Hence, the clonal lines were somatic cell hybrids and not LM(TK-) cell revertants. The experiments demonstrate that chick erythrocyte cytosol dT kinase can be activated in heterokaryons and in hybrid cells, most likely as a result of functions supplied by mouse fibroblast cells.

A T-Cell Marker in Mouse Fibroblast × T-Lymphocyte Somatic Cell Hybrids

2008 ◽  
Vol 6 (12) ◽  
pp. 1333-1337 ◽  
Author(s):  
N. SARAVIA ◽  
R. DEMARS ◽  
B. DUNLAP ◽  
F. H. BACH
Keyword(s):  
T Cell ◽  
Somatic Cell ◽  
T Lymphocyte ◽  
Mouse Fibroblast ◽  
Cell Marker ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids
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