The development of chick-embryo-lethal-orphan (CELO) virus T and V antigens in lytically infected chick kidney cells

1971 ◽  
Vol 7 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Jeffrey Anderson ◽  
Kenneth J. McCormick ◽  
Wayne A. Stenback ◽  
John J. Trentin
Keyword(s):  
Chick Embryo ◽  
Kidney Cells ◽  
Celo Virus ◽  
Chick Kidney

RELATIONS ENTRE LES PROPRIÉTÉS CYTOPATHOGÉNIQUES, HÉMADSORBANTES ET HÉMAGGLUTINANTES DU VIRUS DE L'INFLUENZA

1961 ◽  
Vol 7 (3) ◽  
pp. 347-353 ◽  
Author(s):  
A. Boudreault ◽  
V. Pavilanis
Keyword(s):  
Influenza Virus ◽  
Chick Embryo ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Neutral Ph ◽  
Virus Strains ◽  
Chick Kidney

Quantitative relations between hemadsorption, hemagglutination, and cytopathogenicity of influenza virus strains have been studied. The influenza virus has been cultivated on monkey kidney, chick kidney, and chick embryo cells.It was shown that hemadsorption can also be used for titration of influenza virus adapted on tissue culture.This technic is a reliable and sensitive one. It was also found that the growth of influenza virus is better in a medium of neutral pH and that chicks' age is not an important factor in the multiplication of the virus on chick kidney cells.

A Culture Technique Using Marine Fish Kidney to Obtain Chromosomes

1979 ◽  
Vol 36 (4) ◽  
pp. 458-461 ◽  
Author(s):  
Eun Ho Park ◽  
Sang Dai Park
Keyword(s):  
Chick Embryo ◽  
Marine Fish ◽  
Culture Technique ◽  
Kidney Cells ◽  
High Concentration ◽  
Embryo Extract ◽  
Key Words Kidney ◽  
Chick Embryo Extract ◽  
Conger Eel

A relatively simple and reliable in vitro method for marine fish chromosome study was developed. The addition of 10% chick embryo extract to serum-supplemented Eagle's minimum essential medium with high concentration of NaCl resulted in marked growth of kidney cells in the marine conger eel (Astroconger myriaster) after activation by phytohemagglutinin (PHA). Culture medium without chick embryo extract or PHA and/or with normal concentration of NaCl did not induce substantial growth. In contrast to reports by others, humidified culture was not required for excellent cell growth of these teleost kidney cells. Numerous metaphases unmarred by overlapping chromosomes were recovered and excellent karyograms were available for detailed karyotype analysis. Key words: kidney, culture, marine fish, chromosome

scholarly journals INHIBITION OF RELEASE OF VACCINIA VIRUS BY N1-ISONICOTINOYL-N2-3-METHYL-4-CHLOROBENZOYLHYDRAZINE

1969 ◽  
Vol 129 (4) ◽  
pp. 795-808 ◽  
Author(s):  
Nobuo Kato ◽  
Hans J. Eggers ◽  
Heinrich Rolly
Keyword(s):  
Chick Embryo ◽  
Vaccinia Virus ◽  
Inhibitory Effect ◽  
Virus Multiplication ◽  
Kidney Cells ◽  
Virus Release ◽  
Single Cycle ◽  
Infected Cells ◽  
Embryo Fibroblasts ◽  
Intracellular Virus

N1-isonicotinoyl-N2-3-methyl-4-chlorobenzoylhydrazine (IMCBH) is a selective inhibitor of vaccinia virus multiplication. In concentrations up to 50 µg/ml, IMCBH causes neither toxic morphologic changes, nor does it inhibit the multiplication of cells. Viruses other than vaccinia are not affected by IMCBH. The virus-inhibitory effect of IMCBH is dependent on the type of host cell used, i.e., the compound is effective in chick embryo fibroblasts and monkey kidney cells but not in L cells. IMCBH does not exhibit any protecting effect on vaccinia virus-infected mice or rabbits. IMCBH interferes with virus release: in single cycle experiments in chick embryo fibroblasts, IMCBH strongly blocks the release of vaccinia virus at concentrations as low as 3 µg/ml, while intracellular virus synthesis is hardly affected. Viral cytopathic changes are completely suppressed by IMCBH within the span of a single cycle infection, although extensive changes eventually occur. By inhibiting virus release from initially infected cells, IMCBH markedly inhibits the multiplication of vaccinia virus in cell cultures infected at low virus/ cell multiplicities. IMCBH does not inhibit the early toxic cytopathic changes induced by large inocula of vaccinia virus in BHK21 cells.

Chick embryo lethal orphan (CELO) virus: Some physical and immunological properties

Virology ◽  
1971 ◽  
Vol 44 (2) ◽  
pp. 418-424 ◽  
Author(s):  
C.W. Potter ◽  
J.S. Oxford ◽  
Jean C. Downie ◽  
Margaret M. Attwood ◽  
R.D. Hardy
Keyword(s):  
Chick Embryo ◽  
Immunological Properties ◽  
Celo Virus
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