scholarly journals Resveratrol, a multitargeted agent, can enhance antitumor activity of gemcitabinein vitroand in orthotopic mouse model of human pancreatic cancer

2010 ◽  
pp. NA-NA ◽  
Author(s):  
Kuzhuvelil B. Harikumar ◽  
Ajaikumar B. Kunnumakkara ◽  
Gautam Sethi ◽  
Parmeswaran Diagaradjane ◽  
Preetha Anand ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Antitumor Activity ◽  
Mouse Model ◽  
Human Pancreatic Cancer ◽  
Orthotopic Mouse Model

scholarly journals Curcumin Inhibits Tumor Growth and Angiogenesis in an Orthotopic Mouse Model of Human Pancreatic Cancer

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Sabrina Bimonte ◽  
Antonio Barbieri ◽  
Giuseppe Palma ◽  
Antonio Luciano ◽  
Domenica Rea ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Mouse Model ◽  
Cell Signalling ◽  
Malignant Neoplasm ◽  
Human Pancreatic Cancer ◽  
Antitumor Effects ◽  
Orthotopic Mouse Model ◽  
Toxic Agents

Pancreatic cancer is a malignant neoplasm originating from transformed cells arising in tissues forming the pancreas. The best chemotherapeutic agent used to treat pancreatic cancer is the gemcitabine. However, gemcitabine treatment is associated with many side effects. Thus novel strategies involving less toxic agents for treatment of pancreatic cancer are necessary. Curcumin is one such agent that inhibits the proliferation and angiogenesis of a wide variety of tumor cells, through the modulation of many cell signalling pathways. In this study, we investigated whether curcumin plays antitumor effects in MIA PaCa-2 cells.In vitrostudies showed that curcumin inhibits the proliferation and enhances apoptosis of MIA PaCa-2 cells. To test whether the antitumor activity of curcumin is also observedin vivo, we generated an orthotopic mouse model of pancreatic cancer by injection of MIA PaCa-2 cells in nude mice. We placed mice on diet containing curcumin at 0.6% for 6 weeks. In these treated mice tumors were smaller with respect to controls and showed a downregulation of the transcription nuclear factor NF-κB and NF-κB-regulated gene products. Overall, our data indicate that curcumin has a great potential in treatment of human pancreatic cancer through the modulation of NF-κB pathway.

Ultrasound-guided direct delivery of 3-bromopyruvate blocks tumor progression in an orthotopic mouse model of human pancreatic cancer

2013 ◽  
Vol 8 (2) ◽  
pp. 145-151 ◽  
Author(s):  
Shinichi Ota ◽  
Jean-Francois H. Geschwind ◽  
Manon Buijs ◽  
Joost W. Wijlemans ◽  
Byung Kook Kwak ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Mouse Model ◽  
Tumor Progression ◽  
Human Pancreatic Cancer ◽  
Ultrasound Guided ◽  
Orthotopic Mouse Model ◽  
Direct Delivery

scholarly journals HGF/c-Met Inhibition as Adjuvant Therapy Improves Outcomes in an Orthotopic Mouse Model of Pancreatic Cancer

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2763
Author(s):  
Tony C. Y. Pang ◽  
Zhihong Xu ◽  
Alpha Raj Mekapogu ◽  
Srinivasa Pothula ◽  
Therese Becker ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Progression ◽  
Stellate Cell ◽  
Smooth Muscle Actin ◽  
Primary Tumour ◽  
Human Pancreatic Cancer ◽  
Pancreatic Stellate Cell ◽  
Orthotopic Mouse Model ◽  
Pancreatic Cancer Cells ◽  
Angiogenic Effect

Background: Inhibition of hepatocyte growth factor (HGF)/c-MET pathway, a major mediator of pancreatic stellate cell (PSC)−PC cell interactions, retards local and distant cancer progression. This study examines the use of this treatment in preventing PC progression after resection. We further investigate the postulated existence of circulating PSCs (cPSCs) as a mediator of metastatic PC. Methods: Two orthotopic PC mouse models, produced by implantation of a mixture of luciferase-tagged human pancreatic cancer cells (AsPC-1), and human PSCs were used. Model 1 mice underwent distal pancreatectomy 3-weeks post-implantation (n = 62). One-week post-resection, mice were randomised to four treatments of 8 weeks: (i) IgG, (ii) gemcitabine (G), (iii) HGF/c-MET inhibition (HiCi) and (iv) HiCi + G. Tumour burden was assessed longitudinally by bioluminescence. Circulating tumour cells and cPSCs were enriched by filtration. Tumours of Model 2 mice progressed for 8 weeks prior to the collection of primary tumour, metastases and blood for single-cell RNA-sequencing (scRNA-seq). Results: HiCi treatments: (1) reduced both the risk and rate of disease progression after resection; (2) demonstrated an anti-angiogenic effect on immunohistochemistry; (3) reduced cPSC counts. cPSCs were identified using immunocytochemistry (α-smooth muscle actin+, pan-cytokeratin−, CD45−), and by specific PSC markers. scRNA-seq confirmed the existence of cPSCs and identified potential genes associated with development into cPSCs. Conclusions: This study is the first to demonstrate the efficacy of adjuvant HGF/c-Met inhibition for PC and provides the first confirmation of the existence of circulating PSCs.

A Novel Nude Mouse Model of Liver Metastasis and Peritoneal Dissemination From the Same Human Pancreatic Cancer Line

Pancreas ◽  
2002 ◽  
Vol 24 (3) ◽  
pp. 242-250 ◽  
Author(s):  
Hidefumi Nishimori ◽  
Takahiro Yasoshima ◽  
Fumitake Hata ◽  
Ryuichi Denno ◽  
Yoshiyuki Yanai ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Liver Metastasis ◽  
Mouse Model ◽  
Nude Mouse ◽  
Peritoneal Dissemination ◽  
Human Pancreatic Cancer ◽  
Nude Mouse Model ◽  
Cancer Line

Luteolin and Gemcitabine Protect Against Pancreatic Cancer in an Orthotopic Mouse Model

Pancreas ◽  
2015 ◽  
Vol 44 (1) ◽  
pp. 144-151 ◽  
Author(s):  
Jodee L. Johnson ◽  
Vermont P. Dia ◽  
Matthew Wallig ◽  
Elvira Gonzalez de Mejia
Keyword(s):  
Pancreatic Cancer ◽  
Mouse Model ◽  
Orthotopic Mouse Model
Sign in / Sign up

Export Citation Format

Share Document