scholarly journals Sperm protein 17 is a novel marker for predicting cisplatin response in esophageal squamous cancer cell lines

2010 ◽  
pp. NA-NA ◽  
Author(s):  
Tasneem Kausar ◽  
Aarif Ahsan ◽  
Md. Raghibul Hasan ◽  
Lin Lin ◽  
David G. Beer ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Sperm Protein ◽  
Squamous Cancer ◽  
Esophageal Squamous Cancer ◽  
Sperm Protein 17

scholarly journals Quantitative analysis of cisplatin sensitivity of human esophageal squamous cancer cell lines using in-air micro-PIXE

2010 ◽  
Vol 101 (6) ◽  
pp. 1487-1492 ◽  
Author(s):  
Naritaka Tanaka ◽  
Hitoshi Kimura ◽  
Ahmad Faried ◽  
Makoto Sakai ◽  
Akihiko Sano ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Squamous Cancer ◽  
Cisplatin Sensitivity ◽  
Esophageal Squamous Cancer

scholarly journals Zidovudine, abacavir and lamivudine increase the radiosensitivity of human esophageal squamous cancer cell lines

2016 ◽  
Vol 36 (1) ◽  
pp. 239-246 ◽  
Author(s):  
XUAN CHEN ◽  
CONG WANG ◽  
SHANGHUI GUAN ◽  
YUAN LIU ◽  
LIHUI HAN ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Squamous Cancer ◽  
Esophageal Squamous Cancer

scholarly journals Human Telomerase Reverse Transcriptase as a Therapeutic Target of Dihydroartemisinin for Esophageal Squamous Cancer

2021 ◽  
Vol 12 ◽  
Author(s):  
Qingrong Li ◽  
Qiang Ma ◽  
Lei Xu ◽  
Chuanli Gao ◽  
Lihua Yao ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Human Telomerase Reverse Transcriptase ◽  
Squamous Cancer ◽  
And Migration ◽  
Esophageal Squamous Cancer ◽  
Proliferation And Migration

Objective: To elucidate the oncogenic role of human telomerase reverse transcriptase (hTERT) in esophageal squamous cancer and unravel the therapeutic role and molecular mechanism of dihydroartemisinin (DHA) by targeting hTERT.Methods: The expression of hTERT in esophageal squamous cancer and the patients prognosis were analyzed by bioinformatic analysis from TCGA database, and further validated with esophageal squamous cancer tissues in our cohort. The Cell Counting Kit-8 (CCK8) and colony formation assay were used to evaluate the proliferation of esophageal squamous cancer cell lines (Eca109, KYSE150, and TE1) after hTERT overexpression or treated with indicated concentrations of DHA. Transwell migration assay and scratch assay were employed to determine the migration abilities of cancer cells. Fluorescence microscopy and flow cytometry were conducted to measure the intracellular reactive oxygen species (ROS) levels in cancer cells after treated with DHA. Moreover, RT-PCR and Western blot were performed to test the alteration of associated genes on mRNA and protein level in DHA treated esophageal squamous cancer cell lines, respectively. Furthermore, tumor-bearing nude mice were employed to evaluate the anticancer effect of DHA in vivo.Results: We found that hTERT was significantly upregulated in esophageal squamous cancer both from TCGA database and our cohort also. Overexpression of hTERT evidently promoted the proliferation and migration of esophageal squamous cancer cells in vitro. Moreover, DHA could significantly inhibit the proliferation and migration of esophageal cancer cell lines Eca109, KYSE150, and TE1 in vitro, and significantly down-regulate the expression of hTERT on both mRNA and protein level in a time- and dose-dependent manner as well. Further studies showed that DHA could induce intracellular ROS production in esophageal cancer cells and down-regulate SP1 expression, a transcription factor that bound to the promoter region of hTERT gene. Moreover, overexpression of SP1 evidently promoted the proliferation and migration of Eca109 and TE1 cells. Intriguingly, rescue experiments showed that inhibiting ROS by NAC alleviated the downregulation of SP1 and hTERT in cells treated with DHA. Furthermore, overexpression of SP1 or hTERT could attenuate the inhibition effect of DHA on the proliferation and migration of Eca109 cells. In tumor-bearing nude mice model, DHA significantly inhibited the growth of esophageal squamous cancer xenografts, and downregulated the expression of SP1 and hTERT protein, while no side effects were observed from heart, kidney, liver, and lung tissues by HE stain.Conclusion: hTERT plays an oncogenic role in esophageal squamous cancer and might be a therapeutic target of DHA through regulating ROS/SP1 pathway.

Novel Curcumin Inspired Antineoplastic 1-Sulfonyl-4-Piperidones: Design, Synthesis and Molecular Modeling Studies

2019 ◽  
Vol 19 (8) ◽  
pp. 1069-1078 ◽  
Author(s):  
Nehmedo G. Fawzy ◽  
Siva S. Panda ◽  
Walid Fayad ◽  
May A. El-Manawaty ◽  
Aladdin M. Srour ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Cell Lines ◽  
Cancer Cell ◽  
Computational Models ◽  
Sulfonyl Chloride ◽  
Antitumor Agent ◽  
Cancer Cell Lines ◽  
Biological Properties ◽  
Qsar Studies ◽  
Squamous Cancer

Background: Curcumin is a well-known example of plant origin exhibiting promising diverse biological properties such as, anti-inflammatory and antitumor as well as poor pharmacokinetic/pharmacodynamic properties. This is why effective agents based on its chemical scaffold were explored. Methods: A set of 3,5-bis(ylidene)-1-(alkylsulfonyl)piperidin-4-ones were synthesized in excellent yield (80- 96%) through dehydrohalogenation reaction of 3,5-bis(ylidene)-4-piperidinones with the corresponding alkane sulfonyl chloride in the presence of triethylamine. Antiproliferative properties of the synthesized compounds (dienone/curcumin inspired analogues) were studied by the standard MTT technique. Results: Most of the synthesized compounds revealed antiproliferative properties against HCT116 (colon) and A431 (skin/squamous) cancer cell lines with IC50 values at sub-micromolar level. Compound 36 also exhibited potency against MCF7 (breast) and A549 (lung) cancer cell lines (IC50 = 2.23, 4.27µM, respectively) higher than that of the reference standards (IC50 = 3.15, 5.93µM for 5-fluorouracil and doxorubicin against MCF7 and A549 cell lines, respectively). Cytotoxic properties of the synthesized compounds against non-cancer RPE1 cell line supported the safety profile of the effective agents against normal cells. Molecular modeling (3Dpharmacophore and 2D-QSAR) studies validated the observed bio-properties and explained the parameters governing activity. Inhibitory properties of compounds 27 and 29 (representative examples of the promising antiproliferative agents synthesized) supported their mode of action against topoisomerase IIα Conclusion: The synthesized scaffold is a promising antitumor agent (with special selectivity against colon and skin/squamous cancer cell lines) so, it can be considered for further investigation and development of highly effective hits/leads based on the computational models obtained.

scholarly journals Involvement of deterioration in S100C/A11-mediated pathway in resistance of human squamous cancer cell lines to TGFβ-induced growth suppression

2007 ◽  
Vol 85 (7) ◽  
pp. 753-762 ◽  
Author(s):  
Hiroyuki Sonegawa ◽  
Takamasa Nukui ◽  
Dai-Wei Li ◽  
Mikiro Takaishi ◽  
Masakiyo Sakaguchi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Growth Suppression ◽  
Squamous Cancer
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