scholarly journals TGFβ1 and TGFα contrarily affect alveolar survival and tumorigenesis in mouse mammary epithelium

2006 ◽  
Vol 120 (3) ◽  
pp. 493-499 ◽  
Author(s):  
Brian W. Booth ◽  
Chamelli Jhappan ◽  
Glenn Merlino ◽  
Gilbert H. Smith
Keyword(s):  
Mammary Epithelium ◽  
Mouse Mammary Epithelium

Prolactin effects on ion transport across cultured mouse mammary epithelium

1981 ◽  
Vol 240 (3) ◽  
pp. C110-C115 ◽  
Author(s):  
C. A. Bisbee
Keyword(s):  
Cell Cultures ◽  
Short Circuit ◽  
Collagen Gel ◽  
Mammary Epithelium ◽  
Short Circuit Current ◽  
Sodium Absorption ◽  
Collagen Gels ◽  
Mouse Mammary Epithelium

Prolactin is a known osmoregulatory hormone in lower vertebrates, and recent evidence indicates that this hormone modulates ionic concentrations in milk. In an ultrastructurally and biochemically differentiated primary cell culture system in which mouse mammary epithelium is maintained on floating collagen gels, prolactin causes an increase in short-circuit current (Isc) of monolayers of cells derived from midpregnant (24.6 to 48.0 microA . cm-2) and lactating (10.4 to 16.1 microA . cm-2) glands. Transepithelial potential differences (basal side ground) average about -12 mV and are similar to those seen in vivo. Prelactating mammary epithelial cell cultures have transepithelial resistances ranging from 374 omega . cm2 (prolactin present) to 507 omega . cm2 (prolactin absent), and lactating cell cultures have resistances averaging almost 1,000 omega . cm2. Prolactin effects require at most one day of culture maintenance in prolactin-containing medium, and the effects are not due to known contamination of prolactin preparations with arginine vasopressin or growth hormone. Medium concentrations of prolactin as low as 1 ng/ml can elicit these effects. In prelactating cell cultures not treated with prolactin, the Isc is equal to the rate of sodium absorption. Prolactin increases sodium absorption fourfold but increases Isc only twofold. Clearly, prolactin induces other active transport; neither potassium nor chloride movements can account for this additional transport. Resistance values, current-voltage plots, and permeability coefficients indicate that in vitro mammary epithelium is a moderately “tight” tissue. Comparisons with intact glands indicate that in vitro mammary epithelium closely resembles its in vivo counterpart. Floating collagen gel cultures appear suitable for elucidating transport properties in cellularly heterogeneous and structurally complex mammalian tissues.

scholarly journals Biogenesis and function of mouse mammary epithelium depends on the presence of functional α-catenin

2004 ◽  
Vol 121 (1) ◽  
pp. 91-99 ◽  
Author(s):  
Rashmi V Nemade ◽  
Brian Bierie ◽  
Masahiro Nozawa ◽  
Celine Bry ◽  
Gilbert H Smith ◽  
...  
Keyword(s):  
Mammary Epithelium ◽  
And Function ◽  
Mouse Mammary Epithelium
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