Isoform-specific expression of 14-3-3 proteins in human lung cancer tissues

2004 ◽  
Vol 113 (3) ◽  
pp. 359-363 ◽  
Author(s):  
Wenqing Qi ◽  
Xiaobing Liu ◽  
Dianhua Qiao ◽  
Jesse D. Martinez
Keyword(s):  
Lung Cancer ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Specific Expression ◽  
Cancer Tissues

scholarly journals POU2F2 promotes the proliferation and motility of lung cancer cells by activating AGO1

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ronggang Luo ◽  
Yi Zhuo ◽  
Quan Du ◽  
Rendong Xiao
Keyword(s):  
Lung Cancer ◽  
Tumor Growth ◽  
Cancer Cells ◽  
Cancer Progression ◽  
Human Lung ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
Cancer Tissues

Abstract Background To detect and investigate the expression of POU domain class 2 transcription factor 2 (POU2F2) in human lung cancer tissues, its role in lung cancer progression, and the potential mechanisms. Methods Immunohistochemical (IHC) assays were conducted to assess the expression of POU2F2 in human lung cancer tissues. Immunoblot assays were performed to assess the expression levels of POU2F2 in human lung cancer tissues and cell lines. CCK-8, colony formation, and transwell-migration/invasion assays were conducted to detect the effects of POU2F2 and AGO1 on the proliferaion and motility of A549 and H1299 cells in vitro. CHIP and luciferase assays were performed for the mechanism study. A tumor xenotransplantation model was used to detect the effects of POU2F2 on tumor growth in vivo. Results We found POU2F2 was highly expressed in human lung cancer tissues and cell lines, and associated with the lung cancer patients’ prognosis and clinical features. POU2F2 promoted the proliferation, and motility of lung cancer cells via targeting AGO1 in vitro. Additionally, POU2F2 promoted tumor growth of lung cancer cells via AGO1 in vivo. Conclusion We found POU2F2 was highly expressed in lung cancer cells and confirmed the involvement of POU2F2 in lung cancer progression, and thought POU2F2 could act as a potential therapeutic target for lung cancer.

Application of cryobiopsy to morphological and immunohistochemical analyses of xenografted human lung cancer tissues and functional blood vessels

Cancer ◽  
2008 ◽  
Vol 113 (5) ◽  
pp. 1068-1079 ◽  
Author(s):  
Nobuhiko Ohno ◽  
Nobuo Terada ◽  
Yuqin Bai ◽  
Sei Saitoh ◽  
Tadao Nakazawa ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Blood Vessels ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Cancer Tissues ◽  
Immunohistochemical Analyses

scholarly journals Comprehensive Analysis of the Expression and Prognosis for IRXs in Non-small Cell Lung Cancer

2020 ◽  
Author(s):  
Qiongzi Wang ◽  
Xueshan Qiu
Keyword(s):  
Lung Cancer ◽  
Human Lung ◽  
Expression Patterns ◽  
Human Lung Cancer ◽  
Prognostic Indicators ◽  
Normal Lung ◽  
Kaplan Meier ◽  
Go Enrichment ◽  
Cancer Tissues ◽  
Kaplan Meier Plotter

Abstract Iroquoishomeobox transcription factor family （IRXs）have been increasingly reported to play roles in suppressing or promoting a variety of cancers, however, little is known about their expression and prognostic value in terms of human lung cancer. In this study, Oncomine, GEPIA, Kaplan-Meier plotter, and cBioPortal databases were used to analyze the different expression patterns and prognostic values of six IRXs in NSCLC and examine their related functions and pathways using GO enrichment. Compared with normal lung cancer tissues, the expression of IRX1 and IRX2 in NSCLC tissues was significantly lower and was positively correlated with the 10-year survival rate of patients. Higher expression of IRX4 was related to terminal tumors, and suggested a poor prognosis. It was also found that IRXs may play a tumor-suppressive role in the localization of cytoplasm in NSCLC, while localization in the nucleus suggests a more malignant behavior. Together these results suggest that IRX1 and IRX2 may be prognostic indicators of LUAD, and that IRX4 could be a potential target for LUAD treatment.

Increased Expression of Phospholipase C-gamma1 Activator Protein, AHNAK in Human Lung Cancer Tissues

1999 ◽  
Vol 47 (3) ◽  
pp. 347
Author(s):  
Yoon Jung Oh ◽  
Chun Seong Park ◽  
So Yeon Choi ◽  
Seong Cheoll Cheong ◽  
Sun Min Lee ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Phospholipase C ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Activator Protein ◽  
Cancer Tissues

Oligoclonal T lymphocytes infiltrating human lung cancer tissues

1991 ◽  
Vol 47 (5) ◽  
pp. 654-658 ◽  
Author(s):  
Ichiro Yoshino ◽  
Tokujiro Yano ◽  
Yasunobu Yoshikai ◽  
Mitsuhiro Murata ◽  
Keizo Sugimachi ◽  
...  
Keyword(s):  
Lung Cancer ◽  
T Lymphocytes ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Cancer Tissues

HPLC quantification of 5-hydroxyeicosatetraenoic acid in human lung cancer tissues

2009 ◽  
Vol 23 (8) ◽  
pp. 817-821 ◽  
Author(s):  
Mikell Paige ◽  
Mary S. Saprito ◽  
Dorothy A. Bunyan ◽  
Y. Michael Shim
Keyword(s):  
Lung Cancer ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Hydroxyeicosatetraenoic Acid ◽  
Cancer Tissues

scholarly journals Human Lung-Resident Macrophages Express and Are Targets of Thymic Stromal Lymphopoietin in the Tumor Microenvironment

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 2012
Author(s):  
Mariantonia Braile ◽  
Alfonso Fiorelli ◽  
Daniela Sorriento ◽  
Rosa Maria Di Crescenzo ◽  
Maria Rosaria Galdiero ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Human Lung ◽  
Short Form ◽  
Biological Effects ◽  
Thymic Stromal Lymphopoietin ◽  
Human Lung Cancer ◽  
Blood Monocytes ◽  
Adaptive Immune Cells ◽  
Angiopoietin 2 ◽  
Cancer Tissues

Thymic stromal lymphopoietin (TSLP) is a pleiotropic cytokine highly expressed by epithelial cells and several innate and adaptive immune cells. TSLP exerts its biological effects by binding to a heterodimeric complex composed of TSLP receptor (TSLPR) and IL-7Rα. In humans, there are two TSLP isoforms: the short form (sfTSLP), constitutively expressed, and the long form (lfTSLP), which is upregulated in inflammation. TSLP has been implicated in the induction and progression of several experimental and human cancers. Primary human lung macrophages (HLMs), monocyte-derived macrophages (MDMs), and peripheral blood monocytes consitutively expressed sfTSLP mRNA. Incubation of HLMs, MDMs, and monocytes with lipopolysaccharide (LPS) or IL-4, but not with IL-13, induced TSLP release from HLMs. LPS, but not IL-4 or IL-13, induced CXCL8 release from HLMs. LPS, IL-4 alone or in combination with IL-13, induced the expression of lfTSLP, but not of sfTSLP from HLMs. Preincubation of HLMs with IL-4, alone or in combination with IL-13, but not IL-13 alone, synergistically enhanced TSLP release from LPS-activated macrophages. By contrast, IL-4, alone or in combination with IL-13, inhibited LPS-induced CXCL8 release from HLMs. Immunoreactive TSLP was detected in lysates of HLMs, MDMs, and monocytes. Incubation of HLMs with TSLP induced the release of proinflammatory (TNF-α), angiogenic (VEGF-A, angiopoietin 2), and lymphangiogenic (VEGF-C) factors. TSLP, TSLPR, and IL-7Rα were expressed in intratumoral and peritumoral areas of human lung cancer. sfTSLP and lfTSLP mRNAs were differentially expressed in peritumoral and intratumoral lung cancer tissues. The TSLP system, expressed in HLMs, MDMs, and monocytes, could play a role in chronic inflammatory disorders including lung cancer.

Sign in / Sign up

Export Citation Format

Share Document