scholarly journals Selection of high affinity human neutralizing antibodies to VEGFR2 from a large antibody phage display library for antiangiogenesis therapy

2001 ◽  
Vol 97 (3) ◽  
pp. 393-399 ◽  
Author(s):  
Dan Lu ◽  
Xenia Jimenez ◽  
Haifan Zhang ◽  
Peter Bohlen ◽  
Larry Witte ◽  
...  
Keyword(s):  
Phage Display ◽  
Neutralizing Antibodies ◽  
Phage Display Library ◽  
High Affinity ◽  
Antibody Phage ◽  
Antibody Phage Display ◽  
Antiangiogenesis Therapy ◽  
Selection Of

scholarly journals Isolation of and Characterization of Neutralizing Antibodies to Covid-19 from a Large Human Naïve scFv Phage Display Library

2020 ◽  
Author(s):  
Andy Q. Yuan ◽  
Likun Zhao ◽  
Lili Bai ◽  
Qingwu Meng ◽  
Zhenguo Wen ◽  
...  
Keyword(s):  
Phage Display ◽  
Neutralizing Antibodies ◽  
Epitope Mapping ◽  
Neutralization Assay ◽  
Phage Display Library ◽  
Antibody Library ◽  
Antibody Phage ◽  
Antibody Phage Display ◽  
Human Receptor

AbstractSARS-CoV-2 (Covid-19) has caused currently ongoing global plague and imposed great challenges to health managing systems all over the world, with millions of infections and hundreds of thousands of deaths. In addition to racing to develop vaccines, neutralizing antibodies (nAbs) to this virus have been extensively sought and are expected to provide another prevention and therapy tool against this frantic pandemic. To offer fast isolation and shortened early development, a large human naïve phage display antibody library, was built and used to screen specific nAbs to the receptor-binding domain, RBD, the key for Covid-19 virus entry through a human receptor, ACE2. The obtained RBD-specific antibodies were characterized by epitope mapping, FACS and neutralization assay. Some of the antibodies demonstrated spike-neutralizing property and ACE2-competitiveness. Our work proved that RBD-specific neutralizing binders from human naïve antibody phage display library are promising candidates to for further Covid-19 therapeutics development.

scholarly journals Neutralizing Antibodies of Botulinum Neurotoxin Serotype A Screened from a Fully Synthetic Human Antibody Phage Display Library

2009 ◽  
Vol 14 (8) ◽  
pp. 991-998 ◽  
Author(s):  
Rui Yu ◽  
Shuang Wang ◽  
Yun-zhou Yu ◽  
Wei-shi Du ◽  
Fang Yang ◽  
...  
Keyword(s):  
Phage Display ◽  
Neutralizing Antibodies ◽  
Botulinum Neurotoxin ◽  
Phage Display Library ◽  
Human Antibody ◽  
Serotype A ◽  
Antibody Phage ◽  
Antibody Phage Display ◽  
Botulinum Neurotoxin Serotype A

The botulinum neurotoxins (BoNTs) produced by Clostridium botulinum are the most poisonous protein substances known. The neutralizing antibodies against botulinum neurotoxin can effectively prevent and cure the toxicosis. Using purified Hc fragments of botulinum neurotoxin serotype A (BoNT/A-Hc) as antigen, 2 specific neutralizing antibodies mapping different epitopes were selected from a fully synthetic human antibody library. The 2 antibodies can effectively inhibit the binding between BoNT/A-Hc and differentiated PC-12 cells in vitro, and the neutralization was evaluated in vivo. Although no single mAb completely protected mice from toxin, they both could prolong time to death when challenged with 20 LD 50s (50% lethal doses) of BoNT/A. When used together, the mAbs completely neutralized 1000 LD50s/mg Ab, suggesting their high neutralizing potency in vivo . The results would lead to further production of neutralizing antibody drugs against BoNT/A. It also proved that it was a quick method to obtain human therapeutic antibodies by selecting from the fully synthetic human antibody phage display library. ( Journal of Biomolecular Screening 2009:991-998)

scholarly journals URSA: ultra rapid selection of antibodies from an antibody phage display library

BioTechniques ◽  
2005 ◽  
Vol 38 (4) ◽  
pp. 536-538 ◽  
Author(s):  
Shannon Hogan ◽  
Kristin Rookey ◽  
Robert Ladner
Keyword(s):  
Phage Display ◽  
Phage Display Library ◽  
Antibody Phage ◽  
Antibody Phage Display ◽  
Selection Of

Construction and Selection of Human Fab Antibody Phage Display Library of Liver Cancer

Hybridoma ◽  
2009 ◽  
Vol 28 (5) ◽  
pp. 341-347 ◽  
Author(s):  
Xuan Shui ◽  
Jian Huang ◽  
Yue-Hui Li ◽  
Ping-Li Xie ◽  
Guan-Cheng Li
Keyword(s):  
Liver Cancer ◽  
Phage Display ◽  
Phage Display Library ◽  
Antibody Phage ◽  
Antibody Phage Display ◽  
Selection Of

scholarly journals Tetanus Neurotoxin Neutralizing Antibodies Screened from a Human Immune scFv Antibody Phage Display Library

Toxins ◽  
2016 ◽  
Vol 8 (9) ◽  
pp. 266 ◽  
Author(s):  
Han Wang ◽  
Rui Yu ◽  
Ting Fang ◽  
Ting Yu ◽  
Xiangyang Chi ◽  
...  
Keyword(s):  
Phage Display ◽  
Neutralizing Antibodies ◽  
Phage Display Library ◽  
Scfv Antibody ◽  
Tetanus Neurotoxin ◽  
Antibody Phage ◽  
Antibody Phage Display ◽  
Human Immune
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