scholarly journals Role of hMLH1 promoter hypermethylation in drug resistance to 5-fluorouracil in colorectal cancer cell lines

2003 ◽  
Vol 106 (1) ◽  
pp. 66-73 ◽  
Author(s):  
Christian N. Arnold ◽  
Ajay Goel ◽  
C. Richard Boland
Keyword(s):  
Colorectal Cancer ◽  
Drug Resistance ◽  
Cell Lines ◽  
Cancer Cell ◽  
Promoter Hypermethylation ◽  
Cancer Cell Lines ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Lines

scholarly journals Hypoxia-Induced miR-675-5p Supports β-Catenin Nuclear Localization by Regulating GSK3-β  Activity in Colorectal Cancer Cell Lines

2020 ◽  
Vol 21 (11) ◽  
pp. 3832 ◽  
Author(s):  
Laura Saieva ◽  
Maria Magdalena Barreca ◽  
Chiara Zichittella ◽  
Maria Giulia Prado ◽  
Marco Tripodi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Nuclear Localization ◽  
Cancer Cell Lines ◽  
Colorectal Cancer Cell ◽  
Non Coding Rna ◽  
Colorectal Cancer Cell Lines ◽  
Gsk 3Β

The reduction of oxygen partial pressure in growing tumors triggers numerous survival strategies driven by the transcription factor complex HIF1 (Hypoxia Inducible Factor-1). Recent evidence revealed that HIF1 promotes rapid and effective phenotypic changes through the induction of non-coding RNAs, whose contribution has not yet been fully described. Here we investigated the role of the hypoxia-induced, long non-coding RNA H19 (lncH19) and its intragenic miRNA (miR-675-5p) into HIF1-Wnt crosstalk. During hypoxic stimulation, colorectal cancer cell lines up-regulated the levels of both the lncH19 and its intragenic miR-675-5p. Loss of expression experiments revealed that miR-675-5p inhibition, in hypoxic cells, hampered β-catenin nuclear localization and its transcriptional activity, while lncH19 silencing did not induce the same effects. Interestingly, our data revealed that miRNA inhibition in hypoxic cells restored the activity of Glycogen Synthase Kinase 3β (GSK-3β) reducing the amount of P-Ser9 kinase, thus unveiling a role of the miR-675-5p in controlling GSK-3β activity. Bioinformatics analyses highlighted the serine/threonine-protein phosphatases PPP2CA, responsible for GSK-3β activation, among the miR-675-5p targets, thus indicating the molecular mediator through which miR-675-5p may control β-catenin nuclear localization. In conclusion, here we demonstrated that the inhibition of the hypoxia-induced non-coding RNA miR-675-5p hampered the nuclear localization of β-catenin by regulating GSK-3β activity, thus proposing the miR-675-5p as a new therapeutic target for the treatment of colorectal cancer.

scholarly journals Promoter hypermethylation of theADAM23gene in colorectal cancer cell lines and cancer tissues

2009 ◽  
Vol 124 (6) ◽  
pp. 1258-1262 ◽  
Author(s):  
Jin-Sung Choi ◽  
Kyung-Hee Kim ◽  
You-Kyung Jeon ◽  
Sung-Hee Kim ◽  
Sang-Geun Jang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Promoter Hypermethylation ◽  
Cancer Cell Lines ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Lines ◽  
Cancer Tissues

scholarly journals Promoter hypermethylation downregulates RUNX3 gene expression in colorectal cancer cell lines

Oncogene ◽  
2004 ◽  
Vol 23 (40) ◽  
pp. 6736-6742 ◽  
Author(s):  
Ja-Lok Ku ◽  
Sung-Bum Kang ◽  
Young-Kyoung Shin ◽  
Hio Chung Kang ◽  
Sung-Hye Hong ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Promoter Hypermethylation ◽  
Cancer Cell Lines ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Lines ◽  
Runx3 Gene

IGF2 knockdown in two colorectal cancer cell lines decreases survival, adhesion and modulates survival-associated genes

Tumor Biology ◽  
2016 ◽  
Vol 37 (9) ◽  
pp. 12485-12495 ◽  
Author(s):  
Michael A. Rogers ◽  
Verena Kalter ◽  
Moritz Strowitzki ◽  
Martin Schneider ◽  
Peter Lichter
Keyword(s):  
Colorectal Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Lines
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