Effects of glutamine on proinflammatory gene expression and activation of nuclear factor kappa B and signal transducers and activators of transcription in TNBS-induced colitis

2008 ◽  
Vol 14 (11) ◽  
pp. 1504-1513 ◽  
Author(s):  
Nelson A. Kretzmann ◽  
Henrique Fillmann ◽  
José L. Mauriz ◽  
Claudio Augusto Marroni ◽  
Norma Marroni ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nuclear Factor Kappa B ◽  
Nuclear Factor ◽  
Signal Transducers ◽  
Nuclear Factor Kappa ◽  
Proinflammatory Gene ◽  
Proinflammatory Gene Expression ◽  
Signal Transducers And Activators

scholarly journals Challenges with Methods for Detecting and Studying the Transcription Factor Nuclear Factor Kappa B (NF-κB) in the Central Nervous System

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1335
Author(s):  
Marina Mostafizar ◽  
Claudia Cortes-Pérez ◽  
Wanda Snow ◽  
Jelena Djordjevic ◽  
Aida Adlimoghaddam ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Signaling Pathways ◽  
Quantitative Methods ◽  
Nuclear Factor Kappa B ◽  
Inflammatory Responses ◽  
Nuclear Factor ◽  
Nuclear Factor Kappa ◽  
Regulated Gene Expression ◽  
Transcription Factor Nuclear Factor

The transcription factor nuclear factor kappa B (NF-κB) is highly expressed in almost all types of cells. NF-κB is involved in many complex biological processes, in particular in immunity. The activation of the NF-κB signaling pathways is also associated with cancer, diabetes, neurological disorders and even memory. Hence, NF-κB is a central factor for understanding not only fundamental biological presence but also pathogenesis, and has been the subject of intense study in these contexts. Under healthy physiological conditions, the NF-κB pathway promotes synapse growth and synaptic plasticity in neurons, while in glia, NF-κB signaling can promote pro-inflammatory responses to injury. In addition, NF-κB promotes the maintenance and maturation of B cells regulating gene expression in a majority of diverse signaling pathways. Given this, the protein plays a predominant role in activating the mammalian immune system, where NF-κB-regulated gene expression targets processes of inflammation and host defense. Thus, an understanding of the methodological issues around its detection for localization, quantification, and mechanistic insights should have a broad interest across the molecular neuroscience community. In this review, we summarize the available methods for the proper detection and analysis of NF-κB among various brain tissues, cell types, and subcellular compartments, using both qualitative and quantitative methods. We also summarize the flexibility and performance of these experimental methods for the detection of the protein, accurate quantification in different samples, and the experimental challenges in this regard, as well as suggestions to overcome common challenges.

Nuclear Factor of Activated T Cell Mediates Proinflammatory Gene Expression in Response to Mechanotransduction

2007 ◽  
Vol 1117 (1) ◽  
pp. 138-142 ◽  
Author(s):  
A. B. CELIL AYDEMIR ◽  
S. LEE ◽  
D. WON KIM ◽  
T. R. GARDNER ◽  
D. PRINCE ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cell ◽  
Nuclear Factor ◽  
Activated T Cell ◽  
Proinflammatory Gene ◽  
Proinflammatory Gene Expression
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