Oligonucleotide Analogues with Integrated Bases and Backbone. Part 32

2014 ◽  
Vol 97 (9) ◽  
pp. 1244-1268 ◽  
Author(s):  
Martina Schulze-Adams ◽  
Bruno Bernet ◽  
David Touboul ◽  
Daniel Egli ◽  
Lorenz Herdeis ◽  
...  
Keyword(s):  
Oligonucleotide Analogues

ChemInform Abstract: Novel Oligonucleotide Analogues Derived from Serine and 4-Hydroxyproline

ChemInform ◽  
2010 ◽  
Vol 30 (44) ◽  
pp. no-no
Author(s):  
V. A. Efimov ◽  
M. V. Choob ◽  
A. A. Buryakova ◽  
O. G. Chakhmakhcheva
Keyword(s):  
Oligonucleotide Analogues

Oligonucleotide Analogues with Integrated Bases and Backbone. Part 27

2011 ◽  
Vol 94 (4) ◽  
pp. 545-577 ◽  
Author(s):  
Lorenz Herdeis ◽  
Bruno Bernet ◽  
Anu Augustine ◽  
Roland E. Kälin ◽  
André W. Brändli ◽  
...  
Keyword(s):  
Oligonucleotide Analogues

Synthesis and Hybridization Properties of Oligonucleotide Analogues Containing Ornithine Backbone Modified with Nucleoalanines

ChemInform ◽  
2004 ◽  
Vol 35 (6) ◽  
Author(s):  
N. Sumbatyan ◽  
A. Maksimenko ◽  
M. Gottikh ◽  
C. Malvy ◽  
G. Korshunova
Keyword(s):  
Oligonucleotide Analogues

scholarly journals Analysis of nuclear glucocorticoid receptor-DNA interaction in aged rat liver

2005 ◽  
Vol 70 (5) ◽  
pp. 705-712 ◽  
Author(s):  
Miroslava Vujcic ◽  
Natasa Terzic ◽  
Aleksandra Ristic-Fira ◽  
Dusan Kanazir ◽  
Sabera Ruzdijic
Keyword(s):  
Dna Interaction ◽  
Binding Activity ◽  
Regulatory Proteins ◽  
Nuclear Proteins ◽  
Middle Aged ◽  
Mobility Shift ◽  
Nuclear Extracts ◽  
Supershift Assay ◽  
Mobility Shift Assay ◽  
Oligonucleotide Analogues

Abstract: In order to contribute to the understanding of mechanisms by which regulatory proteins recognize genetic information stored in DNA, analyses of their interaction with specific nucleotides are usually performed. In this study, the electrophoretic mobility shift assay (EMSA) was applied to analyze the interaction of nuclear proteins from the liver of rats of different age i.e., young (3-month-old), middle- aged (12-month-old) and aged (24-month-old), with radioactively labelled synthetic oligonucleotide analogues, corresponding to GRE. The levels of GRE binding activity were assessed by quantitative densitometric scanning of the autoradiograms. The results showed statistically significant decreasing values of up to 78% and 49% in middle aged and old animals, respectively, compared to young animals (p < 0.05). The specificity of the nuclear proteins-GRE interaction was demonstrated by competition experiments with unlabelled GRE. In a supershift assay, using the antibody BuGR2, it was shown that the GR proteins present in nuclear extracts have a high affinity for the GRE probe. The stabilities of the protein-DNA complexes were analysed and it was concluded that they changed during ageing. .

Synthesis of Oligonucleotide Analogues with Modified Backbones

Synlett ◽  
1993 ◽  
Vol 1993 (09) ◽  
pp. 621-637 ◽  
Author(s):  
Rajender S. Varma
Keyword(s):  
Oligonucleotide Analogues

Oligonucleotide Analogues with Integrated Bases and Backbone. Part 30

2013 ◽  
Vol 96 (7) ◽  
pp. 1235-1265 ◽  
Author(s):  
Lorenz Herdeis ◽  
Siji Thomas ◽  
Bruno Bernet ◽  
Andrea Vasella
Keyword(s):  
Oligonucleotide Analogues

Cell-penetrating-peptide-based delivery of oligonucleotides: an overview

2007 ◽  
Vol 35 (4) ◽  
pp. 775-779 ◽  
Author(s):  
R. Abes ◽  
A.A. Arzumanov ◽  
H.M. Moulton ◽  
S. Abes ◽  
G.D. Ivanova ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
Peptide Delivery ◽  
Cell Penetrating Peptide ◽  
Cellular Internalization ◽  
Structure Activity ◽  
Cell Penetrating ◽  
Transactivator Of Transcription ◽  
Endocytic Vesicles ◽  
Oligonucleotide Analogues ◽  
Phosphorodiamidate Morpholino Oligomers

Cationic CPPs (cell-penetrating peptides) have been used largely for intracellular delivery of low-molecular-mass drugs, biomolecules and particles. Most cationic CPPs bind to cell-associated glycosaminoglycans and are internalized by endocytosis, although the detailed mechanisms involved remain controversial. Sequestration and degradation in endocytic vesicles severely limits the efficiency of cytoplasmic and/or nuclear delivery of CPP-conjugated material. Re-routing the splicing machinery by using steric-block ON (oligonucleotide) analogues, such as PNAs (peptide nucleic acids) or PMOs (phosphorodiamidate morpholino oligomers), has consequently been inefficient when ONs are conjugated with standard CPPs such as Tat (transactivator of transcription), R9 (nona-arginine), K8 (octalysine) or penetratin in the absence of endosomolytic agents. New arginine-rich CPPs such as (R-Ahx-R)4 (6-aminohexanoic acid-spaced oligo-arginine) or R6 (hexa-arginine)–penetratin conjugated to PMO or PNA resulted in efficient splicing correction at non-cytotoxic doses in the absence of chloroquine. SAR (structure–activity relationship) analyses are underway to optimize these peptide delivery vectors and to understand their mechanisms of cellular internalization.

Sign in / Sign up

Export Citation Format

Share Document