Promiscuity in Antibody Catalysis: Esterolytic Activity of the Decarboxylase 21D8

2003 ◽  
Vol 86 (4) ◽  
pp. 1167-1174 ◽  
Author(s):  
Alexander C. Backes ◽  
Kinya Hotta ◽  
Donald Hilvert
Keyword(s):  
Antibody Catalysis ◽  
Esterolytic Activity

Cold Promoted Activation of Factor VII

1972 ◽  
Vol 28 (02) ◽  
pp. 169-181 ◽  
Author(s):  
H Gjønnæss
Keyword(s):  
Low Temperature ◽  
Oral Contraceptives ◽  
Fold Increase ◽  
Factor Vii ◽  
Plasma Kallikrein ◽  
Factor Xii ◽  
Overnight Incubation ◽  
Esterolytic Activity

SummaryThe activating principle (CPA) of the factor VII activation seen in plasmas of women taking oral contraceptives after overnight incubation of the plasmas at 0° C was investigated. The reaction was dependent on low temperature, and factor XII was indispensable. Concomitant with the activation of factor VII a 10–30 fold increase in TAME esterolytic activity was observed together with a near 100 per cent drop in plasma kininogen concentration. The results indicated that the activation of factor VII is linked to activation of the kallikrein system, and that the activator may be plasma kallikrein.

Structural Basis for Antibody Catalysis of a Cationic Cyclization Reaction

2003 ◽  
Vol 329 (1) ◽  
pp. 69-83 ◽  
Author(s):  
Xueyong Zhu ◽  
Andreas Heine ◽  
Frédéric Monnat ◽  
K.N. Houk ◽  
Kim D. Janda ◽  
...  
Keyword(s):  
Cyclization Reaction ◽  
Structural Basis ◽  
Antibody Catalysis ◽  
Cationic Cyclization

The Esterolytic Activity of Poly(1-methyl-4- and -5-vinylimidazole) in Water

1975 ◽  
Vol 8 (4) ◽  
pp. 416-424 ◽  
Author(s):  
C. G. Overberger ◽  
Thomas W. Smith
Keyword(s):  
Esterolytic Activity

Thiol derivatives of cellulose as supports for the immobilization of non-thiol enzymes

1981 ◽  
Vol 46 (7) ◽  
pp. 1693-1700 ◽  
Author(s):  
Peter Gemeiner ◽  
Jiří Zemek
Keyword(s):  
Exchange Reaction ◽  
Proteolytic Activity ◽  
Acetylcholine Esterase ◽  
Mercaptoacetic Acid ◽  
Thiol Groups ◽  
Nucleophilic Reaction ◽  
Disulfide Exchange ◽  
Immobilized Trypsin ◽  
Esterolytic Activity ◽  
Derivatives Of

Thiosulfate derivatives, which can be reduced with mercaptoacetic acid, are suitable intermediates for the preparation of thiol derivatives of polymers. Thiosulfate derivatives of cellulose were prepared via chlorodeoxy- or via 3-chloro-2-hydroxy-propylcellulose, while mercaptodeoxycellulose prepared via chlorodeoxy derivative had more convenient properties for the immobilization of non-thiol enzymes (acetylcholine esterase, butyrylcholine esterase and trypsin). Before immobilization SH groups were introduced into choline esterases by i) reduction of the cystine residues, ii) reaction with methyl 4-mercaptobutyrimidate, and the isothiocyanate groups were introduced into trypsin on reaction with 3-isothiocyanatopropyl 1-isocyanate. The immobilization of the enzymes treated in this way was carried out under the conditions of the oxidation of thiol groups (i), thiol-disulfide exchange reaction (ii), or an addition nucleophilic reaction of isothiocyanates with thiols. In contrast to the proteolytic activity of the immobilized trypsin the esterolytic activity of immobilized choline esterases attained satisfactory values.

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