Transient effect of mossy fiber stimulation on spatial firing of CA3 neurons in familiar and novel environments

Hippocampus ◽  
2020 ◽  
Vol 30 (7) ◽  
pp. 693-702
Author(s):  
Joonyeup Lee ◽  
Chanmee Bae ◽  
Doyun Lee ◽  
Min Whan Jung
Keyword(s):  
Mossy Fiber ◽  
Transient Effect ◽  
Novel Environments ◽  
Ca3 Neurons ◽  
Fiber Stimulation

scholarly journals Transient effect of mossy fiber stimulation on spatial firing of CA3 neurons

2018 ◽  
Author(s):  
Joonyeup Lee ◽  
Miru Yun ◽  
Eunjae Cho ◽  
Jong Won Lee ◽  
Doyun Lee ◽  
...  
Keyword(s):  
Mossy Fiber ◽  
Empirical Test ◽  
Mossy Fibers ◽  
Transient Effect ◽  
Optogenetic Stimulation ◽  
Place Fields ◽  
Freely Moving ◽  
Ca3 Neurons ◽  
Fiber Stimulation ◽  
Stimulation Of

AbstractStrong hippocampal mossy fiber synapses are thought to function as detonators, imposing ‘teaching’ signals onto CA3 neurons during new memory formation. For an empirical test of this long-standing view, we examined effects of stimulating mossy fibers on spatial firing of CA3 neurons in freely-moving mice. We found that optogenetic stimulation of mossy fibers can alter CA3 spatial firing, but their effects are only transient. Spatially restricted mossy fiber stimulation, either congruent or incongruent with CA3 place fields, was more likely to suppress than enhance CA3 neuronal activity. Also, changes in spatial firing induced by optogenetic stimulation reverted immediately upon stimulation termination, leaving CA3 place fields unaltered. Our results do not support the traditional view that mossy fibers impose teaching signals onto CA3 network, and show robustness of established CA3 spatial representations.

scholarly journals Transient effect of mossy fiber stimulation on spatial firing of CA3 neurons

Hippocampus ◽  
2019 ◽  
Vol 29 (7) ◽  
pp. 639-651 ◽  
Author(s):  
Joonyeup Lee ◽  
Miru Yun ◽  
Eunjae Cho ◽  
Jong Won Lee ◽  
Doyun Lee ◽  
...  
Keyword(s):  
Mossy Fiber ◽  
Transient Effect ◽  
Ca3 Neurons ◽  
Fiber Stimulation

Enhanced Ca2+ influx with mossy fiber stimulation in hippocampal CA3 neurons of spontaneously epileptic rats

2001 ◽  
Vol 910 (1-2) ◽  
pp. 199-203 ◽  
Author(s):  
Taku Amano ◽  
Hiroko Amano ◽  
Hiroaki Matsubayashi ◽  
Kumatoshi Ishihara ◽  
Tadao Serikawa ◽  
...  
Keyword(s):  
Mossy Fiber ◽  
Hippocampal Ca3 ◽  
Ca3 Neurons ◽  
Fiber Stimulation

scholarly journals The intellectual disability gene Kirrel3 regulates target-specific mossy fiber synapse development in the hippocampus

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
E Anne Martin ◽  
Shruti Muralidhar ◽  
Zhirong Wang ◽  
Diégo Cordero Cervantes ◽  
Raunak Basu ◽  
...  
Keyword(s):  
Synapse Formation ◽  
Mossy Fiber ◽  
Neuron Activity ◽  
Target Cells ◽  
Synaptic Changes ◽  
Mossy Fiber Synapse ◽  
Ca3 Neurons ◽  
Circuit Function ◽  
Feed Forward Inhibition

Synaptic target specificity, whereby neurons make distinct types of synapses with different target cells, is critical for brain function, yet the mechanisms driving it are poorly understood. In this study, we demonstrate Kirrel3 regulates target-specific synapse formation at hippocampal mossy fiber (MF) synapses, which connect dentate granule (DG) neurons to both CA3 and GABAergic neurons. Here, we show Kirrel3 is required for formation of MF filopodia; the structures that give rise to DG-GABA synapses and that regulate feed-forward inhibition of CA3 neurons. Consequently, loss of Kirrel3 robustly increases CA3 neuron activity in developing mice. Alterations in the Kirrel3 gene are repeatedly associated with intellectual disabilities, but the role of Kirrel3 at synapses remained largely unknown. Our findings demonstrate that subtle synaptic changes during development impact circuit function and provide the first insight toward understanding the cellular basis of Kirrel3-dependent neurodevelopmental disorders.

scholarly journals Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer

2019 ◽  
Author(s):  
Nuno Apóstolo ◽  
Samuel N. Smukowski ◽  
Jeroen Vanderlinden ◽  
Giuseppe Condomitti ◽  
Vasily Rybakin ◽  
...  
Keyword(s):  
Cell Surface ◽  
Pyramidal Neurons ◽  
Mossy Fiber ◽  
Surface Protein ◽  
Guidance Cue ◽  
Hippocampal Ca3 ◽  
Cell Surface Interactions ◽  
Ca3 Pyramidal Neurons ◽  
Ca3 Neurons ◽  
Multiple Ligand

SummarySynaptic diversity is a key feature of neural circuits. The structural and functional diversity of closely spaced inputs converging on the same neuron suggests that cell-surface interactions are essential in organizing input properties. Here, we analyzed the cell-surface protein (CSP) composition of hippocampal mossy fiber (MF) inputs on CA3 pyramidal neurons to identify regulators of MF-CA3 synapse properties. We uncover a rich cell-surface repertoire that includes adhesion proteins, guidance cue receptors, extracellular matrix (ECM) proteins, and uncharacterized CSPs. Interactome screening reveals multiple ligand-receptor modules and identifies ECM protein Tenascin-R (TenR) as a ligand of the uncharacterized neuronal receptor IgSF8. Presynaptic Igsf8 deletion impairs MF-CA3 synaptic architecture and robustly decreases the density of bouton filopodia that provide feedforward inhibition of CA3 neurons. Consequently, loss of IgSF8 increases CA3 neuron excitability. Our findings identify IgSF8 as a regulator of CA3 microcircuit development and suggest that combinations of CSP modules define input identity.

scholarly journals Non-synaptic signaling from cerebellar climbing fibers modulates Golgi cell activity

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Angela K Nietz ◽  
Jada H Vaden ◽  
Luke T Coddington ◽  
Linda Overstreet-Wadiche ◽  
Jacques I Wadiche
Keyword(s):  
Feedback Inhibition ◽  
Mossy Fiber ◽  
Molecular Layer ◽  
Cell Activity ◽  
Climbing Fiber ◽  
Climbing Fibers ◽  
In Vivo Studies ◽  
Golgi Cell ◽  
Golgi Cells ◽  
Fiber Stimulation

Golgi cells are the principal inhibitory neurons at the input stage of the cerebellum, providing feedforward and feedback inhibition through mossy fiber and parallel fiber synapses. In vivo studies have shown that Golgi cell activity is regulated by climbing fiber stimulation, yet there is little functional or anatomical evidence for synapses between climbing fibers and Golgi cells. Here, we show that glutamate released from climbing fibers activates ionotropic and metabotropic receptors on Golgi cells through spillover-mediated transmission. The interplay of excitatory and inhibitory conductances provides flexible control over Golgi cell spiking, allowing either excitation or a biphasic sequence of excitation and inhibition following single climbing fiber stimulation. Together with prior studies of spillover transmission to molecular layer interneurons, these results reveal that climbing fibers exert control over inhibition at both the input and output layers of the cerebellar cortex.

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