scholarly journals Biliary Obstruction Promotes Multilineage Differentiation of Hepatic Stem Cells

2019 ◽  
Vol 3 (8) ◽  
pp. 1137-1150
Author(s):  
Mladen I. Yovchev ◽  
Edward J. Lee ◽  
Waldemar Rodriguez‐Silva ◽  
Joseph Locker ◽  
Michael Oertel
Keyword(s):  
Stem Cells ◽  
Biliary Obstruction ◽  
Hepatic Stem Cells ◽  
Multilineage Differentiation

Easy Isolation, Propagation, Characterization and Multilineage Differentiation of Equine Amniotic Fluid Derived Stem Cells

2020 ◽  
pp. 1-22
Author(s):  
Asm Golam KIBRIA ◽  
Shah NAWAZ ◽  
Özlem ÖZDEN AKKAYA ◽  
Tayfun DİKMEN ◽  
Artay YAĞCI
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
Multilineage Differentiation

scholarly journals Bioinspired One Cell Culture Isolates Highly Tumorigenic and Metastatic Cancer Stem Cells Capable of Multilineage Differentiation

2020 ◽  
Vol 7 (11) ◽  
pp. 2000259
Author(s):  
Hai Wang ◽  
Pranay Agarwal ◽  
Bin Jiang ◽  
Samantha Stewart ◽  
Xuanyou Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Cancer Stem Cells ◽  
Metastatic Cancer ◽  
Multilineage Differentiation

Mesenchymal stem cells induce mature dendritic cells into a novel Jagged-2–dependent regulatory dendritic cell population

Blood ◽  
2009 ◽  
Vol 113 (1) ◽  
pp. 46-57 ◽  
Author(s):  
Bin Zhang ◽  
Rui Liu ◽  
Dan Shi ◽  
Xingxia Liu ◽  
Yuan Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dendritic Cells ◽  
Immune Cells ◽  
Cell Contact ◽  
Multilineage Differentiation ◽  
Immunomodulatory Effects ◽  
Cd11b Expression ◽  
Dendritic Cell Population

Abstract Mesenchymal stem cells (MSCs), in addition to their multilineage differentiation, exert immunomodulatory effects on immune cells, even dendritic cells (DCs). However, whether they influence the destiny of full mature DCs (maDCs) remains controversial. Here we report that MSCs vigorously promote proliferation of maDCs, significantly reduce their expression of Ia, CD11c, CD80, CD86, and CD40 while increasing CD11b expression. Interestingly, though these phenotypes clearly suggest their skew to immature status, bacterial lipopolysaccharide (LPS) stimulation could not reverse this trend. Moreover, high endocytosic capacity, low immunogenicity, and strong immunoregulatory function of MSC-treated maDCs (MSC-DCs) were also observed. Furthermore we found that MSCs, partly via cell-cell contact, drive maDCs to differentiate into a novel Jagged-2–dependent regulatory DC population and escape their apoptotic fate. These results further support the role of MSCs in preventing rejection in organ transplantation and treatment of autoimmune disease.

scholarly journals Low Oxygen Tension Potentiates Proliferation and Stemness but Not Multilineage Differentiation of Caprine Male Germline Stem Cells

2021 ◽  
Author(s):  
Shiva Pratap Singh ◽  
Suresh Dinkar Kharche ◽  
Manisha Pathak ◽  
Ravi Ranjan ◽  
Yogesh Kumar Soni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ex Vivo ◽  
Growth Characteristics ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Germline Stem Cells ◽  
Multilineage Differentiation ◽  
Low Oxygen ◽  
Differentiation Potential

Abstract The milieu of testicular germline stem cells (mGSCs) is characterized as low oxygen (O2) environment, whereas, there in-vitro expansion is typically performed under normoxia (20-21% O2). Here, we evaluated and compared the culture and multilineage differentiation characteristics of enriched (through differential platting and percoll density centrifugation) caprine mGSCs (cmGSCs) under hypoxic (5% O2) and normoxic (21% O2) culture conditions. For this, in addition to growth characteristics and population-doubling time (PDT); viability, proliferation, senescence, and expression of key-markers of adhesion (β-integrin and E-Cadherin) and stemness (OCT-4, THY-1 and UCHL-1) were evaluated and compared under normoxia and hypoxia. Moreover, the extent of multilineage differentiation (neurogenic, adipogenic, and chondrogenic differentiation) was assessed. The survival, viability and proliferation were significantly promoted and PDT was reduced (p < 0.05), thus yielding a higher number of viable cells with larger colonies under hypoxia. Furthermore, expression of stemness and adhesion markers was distinctly increased under lowered O2 condition. Conversely, the presence of differentiated regions and expression of differentiation specific key genes [C/EBPα (adipogenic), nestin and β-tubulin (neurogenic), and COL2A1 (chondrogenic)] were significantly (p < 0.05) reduced under hypoxic conditions. These data demonstrate that culturing cmGSCs under hypoxia augments the growth characteristics, and stemness but not the multilineage differentiation potential of cmGSCs as compared with normoxia. These data are important for the development of robust methodologies for ex-vivo expansion and lineage-committed differentiation of cmGSCs for clinical applications.

scholarly journals Multilineage Differentiation Potential of Stem Cells Derived from Human Dental Pulp after Cryopreservation

2006 ◽  
Vol 0 (0) ◽  
pp. 060928122958008 ◽  
Author(s):  
Weibo Zhang ◽  
X. Frank Walboomers ◽  
Songtao Shi ◽  
Mingwen Fan ◽  
John A. Jansen
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Multilineage Differentiation ◽  
Differentiation Potential ◽  
Human Dental Pulp

scholarly journals Self-Renewal and Multilineage Differentiation In Vitro from Murine Prostate Stem Cells

Stem Cells ◽  
2007 ◽  
Vol 25 (11) ◽  
pp. 2760-2769 ◽  
Author(s):  
Li Xin ◽  
Rita U. Lukacs ◽  
Devon A. Lawson ◽  
Donghui Cheng ◽  
Owen N. Witte
Keyword(s):  
Stem Cells ◽  
Multilineage Differentiation ◽  
Self Renewal

Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance

2008 ◽  
pp. 137-225 ◽  
Author(s):  
Eliane Wauthier ◽  
Eva Schmelzer ◽  
William Turner ◽  
Lili Zhang ◽  
Ed LeCluyse ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ex Vivo ◽  
Hepatic Stem Cells
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