scholarly journals Interferon alfa subtypes and levels of type I interferons in the liver and peripheral mononuclear cells in patients with chronic hepatitis C and controls

Hepatology ◽  
1999 ◽  
Vol 29 (6) ◽  
pp. 1900-1904 ◽  
Author(s):  
Yurdana Castelruiz ◽  
Esther Larrea ◽  
Patricia Boya ◽  
Mar�a-Pilar Civeira ◽  
Jes�s Prieto
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Chronic Hepatitis C ◽  
Mononuclear Cells ◽  
Interferon Alfa ◽  
Type I Interferons ◽  
Type I ◽  
Peripheral Mononuclear Cells

scholarly journals Quantitative Analysis of p40/p46 and p69/p71 Forms of 2′,5′-Oligoadenylate Synthetase mRNA by Competitive PCR and Its Clinical Application

2002 ◽  
Vol 48 (9) ◽  
pp. 1551-1559 ◽  
Author(s):  
Akira Takahashi ◽  
Yoshiaki Iwasaki ◽  
Jiro Miyaike ◽  
Hideaki Taniguchi ◽  
Hiroyuki Shimomura ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Enzymatic Activity ◽  
Chronic Hepatitis C ◽  
Mononuclear Cells ◽  
Type I ◽  
Oligoadenylate Synthetase ◽  
Competitive Pcr ◽  
Peripheral Blood Mononuclear ◽  
Ifn Therapy

Abstract Background: 2′,5′-Oligoadenylate synthetases (2-5AS) are type I interferon (IFN)-induced proteins with antiviral capacity. Three major forms of 2-5AS with distinct enzymatic activities have been described in IFN-treated human cells. We measured distinct forms of 2-5AS mRNA to analyze the relationship with its enzymatic activity and response to IFN therapy in chronic hepatitis C. Methods: We established a method to quantify p40/p46 and p69/p71 forms of 2-5AS mRNA by use of reverse transcription followed by competitive PCR. The 2-5AS mRNA concentrations were measured in peripheral blood mononuclear cells from 40 patients with chronic hepatitis C and 28 control individuals. Results: Reconstitution experiments and comparison with Northern blot analyses revealed that our method accurately and linearly quantified 2-5AS mRNA. 2-5AS mRNA concentrations and 2-5AS enzymatic activity were correlated (P <0.03). Our data demonstrated a correlation in 2-5AS mRNA between p40/p46 and p69/p71 (P <0.02), indicating a similar regulation of the expression of these genes. Our data also demonstrated that pretreatment concentrations of 2-5AS mRNA correlated with responses to IFN therapy in chronic hepatitis C. Conclusions: Our method for measuring 2-5AS mRNA concentrations could provide an important marker for selecting patients for IFN therapy and may be useful for the development of more effective therapeutic strategies for chronic hepatitis C.

scholarly journals Pegylated interferon alfa-2b plus ribavirin for the treatment of chronic hepatitis C genotype 4 in adolescents

2010 ◽  
Vol 9 (2) ◽  
pp. 156-160 ◽  
Author(s):  
Jaber Al Ali ◽  
Salem Owayed ◽  
Wafa’a Al-Qabandi ◽  
Khaled Husain ◽  
Fuad Hasan
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Chronic Hepatitis C ◽  
Pegylated Interferon ◽  
Interferon Alfa ◽  
Genotype 4 ◽  
Pegylated Interferon Alfa

scholarly journals Secondary Hyperpigmentation During Interferon Alfa Treatment for Chronic Hepatitis C Virus Infection

2013 ◽  
Vol 149 (6) ◽  
pp. 675 ◽  
Author(s):  
Katerina Tsilika ◽  
Albert Tran ◽  
Régine Trucchi ◽  
Sinziana Pop ◽  
Rodolphe Anty ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Chronic Hepatitis C ◽  
Interferon Alfa ◽  
Hepatitis C Virus Infection ◽  
Chronic Hepatitis C Virus
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