scholarly journals The polarized hepatic human/rat hybrid WIF 12-1 and WIF-B cells communicate efficientlyin vitro via connexin 32-constituted gap junctions

Hepatology ◽  
1998 ◽  
Vol 28 (1) ◽  
pp. 164-172 ◽  
Author(s):  
Catherine Chaumontet ◽  
Giovanna Mazzoleni ◽  
Catherine Decaens ◽  
Val�rie Bex ◽  
Doris Cassio ◽  
...  
Keyword(s):  
B Cells ◽  
Gap Junctions ◽  
Connexin 32

In vivo modulation of gap junctions and dye coupling between B-cells of the intact pancreatic islet

Diabetes ◽  
1983 ◽  
Vol 32 (9) ◽  
pp. 858-868 ◽  
Author(s):  
P. Meda ◽  
R. L. Michaels ◽  
P. A. Halban ◽  
L. Orci ◽  
J. D. Sheridan
Keyword(s):  
B Cells ◽  
Gap Junctions ◽  
Pancreatic Islet ◽  
Dye Coupling

scholarly journals Gap-Junction Communication Pathways in Germinal Center Reactions

1998 ◽  
Vol 6 (1-2) ◽  
pp. 111-118 ◽  
Author(s):  
Tibor Krenacs ◽  
Martin Rosendaal
Keyword(s):  
B Cells ◽  
Gap Junctions ◽  
B Cell ◽  
Germinal Center ◽  
Lucifer Yellow ◽  
Cell Communication ◽  
Germinal Centers ◽  
Lymphoid Tissues ◽  
Human Tonsil ◽  
Cell Fractions

Intercellular channels called gap junctions enable multicellular organisms to exchange information rapidly between cells. Though gap junctions are held to be ubiquitous in solid tissues, we have only recently found them in the lymphoid organs. Functional direct cell-cell communication has now been confirmed by us and other groups in bone marrow, thymus, and in secondary lymphoid tissues. What functions do they serve in the lymphoreticular system where, so far, only cytokines/growth factors and adhesion molecules have been considered as regulators? Here we show evidence for and refer to published work about functional direct cellcell communication through gap junctions in germinal center reactions and make proposals for their role in the immune response.We found a large amount of the connexin43 (Cx43) gap junctions in the germinal centers of secondary lymphoid follicles. Ultrastructurally and immunohistologically, most of the junctions were detected on the processes of follicular dendritic cells (FDC) enveloping nondividing centrocytes in the light zone of germinal centers where B-cell selection is thought to take place. Further support for this finding came by revealing the Cx43 mRNAin situat the same location as the protein. On antigen challenge, gap junctions appeared on the FDC as they formed meshworks in germinal centers. In order to find out which germinal center cells communicate directly, we separated FDC-rich, low-density, B-cell fractions from human tonsil. In culture, we injected single FDC with the low-molecular-weight fluorescent dye, Lucifer Yellow (Mr 457 Da), which passed between adjacent FDC and sometimes from FDC to B cells.Based on these findings and their assigned functions in other tissues, gap junctions may contribute to germinal center reactions in the following ways: (1) they may regulate follicle pattern formation by controlling FDC growth, (2) they may be involved in FDC-B-cell signaling contributing to the final rescue of selected B cells from apoptosis, and (3) they may enable FDC to work as a functional syncytium providing a cellular internet for integrating germinal center events. Data supporting these interpretations are briefly discussed.

In Vivo Modulation of Gap Junctions and Dye Coupling Between B-Cells of the Intact Pancreatic Islet

Diabetes ◽  
1983 ◽  
Vol 32 (9) ◽  
pp. 858-868 ◽  
Author(s):  
P. Meda ◽  
R. L. Michaels ◽  
P. A. Halban ◽  
L. Orci ◽  
J. D. Sheridan
Keyword(s):  
B Cells ◽  
Gap Junctions ◽  
Pancreatic Islet ◽  
Dye Coupling

scholarly journals Increase of gap junctions between pancreatic B-cells during stimulation of insulin secretion.

1979 ◽  
Vol 82 (2) ◽  
pp. 441-448 ◽  
Author(s):  
P Meda ◽  
A Perrelet ◽  
L Orci
Keyword(s):  
Insulin Secretion ◽  
B Cells ◽  
Gap Junctions ◽  
Freeze Fracture ◽  
Secretory Activity ◽  
Isolated Rat Islets ◽  
Pancreatic B Cells ◽  
Stimulation Of

The development of gap junctions between pancreatic B-cells was quantitatively assessed in freeze-fracture replicas of isolated rat islets under different conditions of insulin secretion. The results show that in resting B-cells, gap junctions are small and scarce but that these junctions increase when insulin secretion is stimulated. Both a short (90 min) stimulation by glucose in vitro and a prolonged (2.5 d) stimulation by glibenclamide in vivo raise the number of gap junctions; in addition, the glibenclamide stimulation causes an increase in the size of individual gap junctions. As a consequence, the total area occupied by gap junctions on the B-cell membrane and the ratio of this area to the cell volume were found significantly increased in the latter condition. The slight increase of these values observed after the glucose stimulation did not reach significance. These data indicate a change of gap junctions during the secretory activity of the pancreatic B-cells. The possibility that the coupling of the cells is affected by the treatment is discussed.

Connexin 32 gap junctions enhance stimulation of glucose output by glucagon and noradrenaline in mouse liver

Hepatology ◽  
1998 ◽  
Vol 28 (6) ◽  
pp. 1616-1620 ◽  
Author(s):  
Frank Stümpel ◽  
Thomas Ott ◽  
Klaus Willecke ◽  
Kurt Jungermann
Keyword(s):  
Gap Junctions ◽  
Mouse Liver ◽  
Connexin 32 ◽  
Glucose Output ◽  
Stimulation Of

scholarly journals Involvement of gap junctions in tumorigenesis: transfection of tumor cells with connexin 32 cDNA retards growth in vivo.

1991 ◽  
Vol 88 (23) ◽  
pp. 10701-10705 ◽  
Author(s):  
B. Eghbali ◽  
J. A. Kessler ◽  
L. M. Reid ◽  
C. Roy ◽  
D. C. Spray
Keyword(s):  
Gap Junctions ◽  
Tumor Cells ◽  
Connexin 32

Connexin 32 and 43 gap junctions differentially modulate tenocyte response to cyclic mechanical load

2006 ◽  
Vol 85 (11) ◽  
pp. 1145-1154 ◽  
Author(s):  
Andrew D. Waggett ◽  
Michael Benjamin ◽  
James R. Ralphs
Keyword(s):  
Gap Junctions ◽  
Mechanical Load ◽  
Connexin 32 ◽  
Cyclic Mechanical Load
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