Letter to the Editor: Can the ratio of serum HBV RNA to DNA reflect the reverse transcription efficiency of viral pgRNA?

Hepatology ◽  
2020 ◽  
Author(s):  
Wanying Li ◽  
Rui Deng ◽  
Shi Liu ◽  
Haitao Guo ◽  
Jian Sun
Keyword(s):  
Reverse Transcription ◽  
Letter To The Editor ◽  
Transcription Efficiency

scholarly journals eEF1A demonstrates paralog specific effects on HIV-1 reverse transcription efficiency

Virology ◽  
2019 ◽  
Vol 530 ◽  
pp. 65-74 ◽  
Author(s):  
Dongsheng Li ◽  
Daniel J. Rawle ◽  
Zhonglan Wu ◽  
Hongping Jin ◽  
Min-Hsuan Lin ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Specific Effects ◽  
Transcription Efficiency ◽  
Hiv 1

scholarly journals Properties of the Reverse Transcription Reaction in mRNA Quantification

2004 ◽  
Vol 50 (3) ◽  
pp. 509-515 ◽  
Author(s):  
Anders Ståhlberg ◽  
Joakim Håkansson ◽  
Xiaojie Xian ◽  
Henrik Semb ◽  
Mikael Kubista
Keyword(s):  
Gene Expression ◽  
Reverse Transcription ◽  
Experimental Accuracy ◽  
Reaction Conditions ◽  
Quantitative Measurements ◽  
Experimental Variation ◽  
Reverse Transcription Reaction ◽  
Transcription Efficiency ◽  
Reverse Transcription Step ◽  
Rna Concentration

Abstract Background: In most measurements of gene expression, mRNA is first reverse-transcribed into cDNA. We studied the reverse transcription reaction and its consequences for quantitative measurements of gene expression. Methods: We used SYBR green I-based quantitative real-time PCR (QPCR) to measure the properties of reverse transcription reaction for the β-tubulin, glyceraldehyde-3-phosphate dehydrogenase, Glut2, CaV1D, and insulin II genes, using random hexamers, oligo(dT), and gene-specific reverse transcription primers. Results: Experimental variation in reverse transcription-QPCR (RT-QPCR) was mainly attributable to the reverse transcription step. Reverse transcription efficiency depended on priming strategy, and the dependence was different for the five genes studied. Reverse transcription yields also depended on total RNA concentration. Conclusions: RT-QPCR gene expression measurements are comparable only when the same priming strategy and reaction conditions are used in all experiments and the samples contain the same total amount of RNA. Experimental accuracy is improved by running samples in (at least) duplicate starting with the reverse transcription reaction.

Letter to the Editor

1978 ◽  
Vol 9 (3) ◽  
pp. 197-200
Author(s):  
Peter B. Smith
Keyword(s):  
Letter To The Editor

Letter to the Editor

1994 ◽  
Vol 3 (1) ◽  
pp. 89-89
Author(s):  
Lawrence I. Shotland
Keyword(s):  
Letter To The Editor

Letter to the Editor

1994 ◽  
Vol 3 (1) ◽  
pp. 87-87
Author(s):  
David Cieliczka
Keyword(s):  
Letter To The Editor

Letter to the Editor

1994 ◽  
Vol 3 (1) ◽  
pp. 92-93
Author(s):  
Larry Engelmann
Keyword(s):  
Letter To The Editor

Letter to the Editor

1994 ◽  
Vol 3 (1) ◽  
pp. 92-92
Author(s):  
Gregory Frazer
Keyword(s):  
Letter To The Editor
Sign in / Sign up

Export Citation Format

Share Document