scholarly journals Physiological ranges of matrix rigidity modulate primary mouse hepatocyte function in part through hepatocyte nuclear factor 4 alpha

Hepatology ◽  
2016 ◽  
Vol 64 (1) ◽  
pp. 261-275 ◽  
Author(s):  
Seema S. Desai ◽  
Jason C. Tung ◽  
Vivian X. Zhou ◽  
James P. Grenert ◽  
Yann Malato ◽  
...  
Keyword(s):  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Matrix Rigidity ◽  
Mouse Hepatocyte ◽  
Primary Mouse ◽  
Hepatocyte Nuclear Factor 4 ◽  
Primary Mouse Hepatocyte

Identification of two splice isoforms of mRNA for mouse hepatocyte nuclear factor 4 (HNF-4)

1995 ◽  
Vol 1260 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Shingo Hata ◽  
Takayuki Inoue ◽  
Kenichi Kosuga ◽  
Takayuki Nakashima ◽  
Toshiro Tsukamoto ◽  
...  
Keyword(s):  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Splice Isoforms ◽  
Mouse Hepatocyte ◽  
Hepatocyte Nuclear Factor 4

scholarly journals A missense mutation in the hepatocyte nuclear factor 4 alpha gene in a UK pedigree with maturity-onset diabetes of the young

Diabetologia ◽  
1997 ◽  
Vol 40 (7) ◽  
pp. 859-862 ◽  
Author(s):  
M. P. Bulman ◽  
M. J. Dronsfield ◽  
T. Frayling ◽  
M. Appleton ◽  
S. C. Bain ◽  
...  
Keyword(s):  
Missense Mutation ◽  
Nuclear Factor ◽  
Maturity Onset Diabetes ◽  
Hepatocyte Nuclear Factor ◽  
Onset Diabetes ◽  
Hepatocyte Nuclear Factor 4 ◽  
Alpha Gene

Tissue-specific regulation of mouse hepatocyte nuclear factor 4 expression

1994 ◽  
Vol 14 (11) ◽  
pp. 7276-7284
Author(s):  
W Zhong ◽  
J Mirkovitch ◽  
J E Darnell
Keyword(s):  
Transcription Factor ◽  
Transgenic Mice ◽  
Transient Transfection ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Distal Enhancer ◽  
Specific Expression ◽  
Tissue Specific ◽  
Hypersensitive Sites ◽  
Hepatocyte Nuclear Factor 4

Hepatocyte nuclear factor 4 (HNF-4) is a liver-enriched transcription factor and a member of the steroid hormone receptor superfamily. HNF-4 is required for the hepatoma-specific expression of HNF-1 alpha, another liver-enriched transcription factor, suggesting the early participation of HNF-4 in development. To prepare for further study of HNF-4 in development, the tissue-specific expression of the mouse HNF-4 gene was studied by analyzing the promoter region for required DNA elements. DNase-hypersensitive sites in the gene in liver and kidney tissues were found in regions both distal and proximal to the RNA start that were absent in tissues in which HNF-4 expression did not occur. By use of reporter constructs in transient-transfection assays and with transgenic mice, a region sufficient to drive liver-specific expression of HNF-4 was identified. While an HNF-1 binding site between bp -98 and -68 played an important role in the hepatoma-specific promoter activity of HNF-4 in transient-transfection assays, it was not sufficient for the liver-specific expression of a reporter gene in transgenic mice. Distal enhancer elements indicated by the presence of DNase I-hypersensitive sites at kb -5.5 and -6.5, while not functional in transient-transfection assays, were required for the correct expression of the mouse HNF-4 gene in animals.

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