scholarly journals Analysis of aberrant pre-messenger RNA splicing resulting from mutations inATP8B1and efficientin vitrorescue by adapted U1 small nuclear RNA

Hepatology ◽  
2015 ◽  
Vol 61 (4) ◽  
pp. 1382-1391 ◽  
Author(s):  
Wendy L. van der Woerd ◽  
Johanna Mulder ◽  
Franco Pagani ◽  
Ulrich Beuers ◽  
Roderick H.J. Houwen ◽  
...  
Keyword(s):  
Rna Splicing ◽  
Messenger Rna ◽  
Small Nuclear Rna ◽  
Nuclear Rna

How Is Precursor Messenger RNA Spliced by the Spliceosome?

2020 ◽  
Vol 89 (1) ◽  
pp. 333-358 ◽  
Author(s):  
Ruixue Wan ◽  
Rui Bai ◽  
Xiechao Zhan ◽  
Yigong Shi
Keyword(s):  
Active Site ◽  
Rna Splicing ◽  
Messenger Rna ◽  
Small Nuclear Rna ◽  
Exon Ligation ◽  
Nuclear Rna ◽  
Catalytic Metal ◽  
Protein Components ◽  
Intron Removal ◽  
Duplex Formation

Splicing of the precursor messenger RNA, involving intron removal and exon ligation, is mediated by the spliceosome. Together with biochemical and genetic investigations of the past four decades, structural studies of the intact spliceosome at atomic resolution since 2015 have led to mechanistic delineation of RNA splicing with remarkable insights. The spliceosome is proven to be a protein-orchestrated metalloribozyme. Conserved elements of small nuclear RNA (snRNA) constitute the splicing active site with two catalytic metal ions and recognize three conserved intron elements through duplex formation, which are delivered into the splicing active site for branching and exon ligation. The protein components of the spliceosome stabilize the conformation of the snRNA, drive spliceosome remodeling, orchestrate the movement of the RNA elements, and facilitate the splicing reaction. The overall organization of the spliceosome and the configuration of the splicing active site are strictly conserved between human and yeast.

scholarly journals Novel models for RNA splicing that involve a small nuclear RNA.

1981 ◽  
Vol 78 (7) ◽  
pp. 4471-4474 ◽  
Author(s):  
Y. Ohshima ◽  
M. Itoh ◽  
N. Okada ◽  
T. Miyata
Keyword(s):  
Rna Splicing ◽  
Small Nuclear Rna ◽  
Nuclear Rna

Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3′ end of H3 messenger RNA

Nature ◽  
1986 ◽  
Vol 323 (6091) ◽  
pp. 777-781 ◽  
Author(s):  
Fred Schaufele ◽  
Gregory M. Gilmartin ◽  
Willi Bannwarth ◽  
Max L. Birnstiel
Keyword(s):  
Messenger Rna ◽  
Small Nuclear Rna ◽  
Base Pairing ◽  
Nuclear Rna ◽  
Compensatory Mutations
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