Caveolin-1 is essential for protecting against binge drinking-induced liver damage through inhibiting reactive nitrogen species

Hepatology ◽  
2014 ◽  
Vol 60 (2) ◽  
pp. 687-699 ◽  
Author(s):  
Lei Gao ◽  
Yingchun Zhou ◽  
Weichao Zhong ◽  
Xiaohua Zhao ◽  
Chun Chen ◽  
...  
Keyword(s):  
Binge Drinking ◽  
Liver Damage ◽  
Reactive Nitrogen Species ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Caveolin 1

scholarly journals Important roles of endothelial caveolin-1 in endothelium-dependent hyperpolarization and ischemic angiogenesis in mice

2019 ◽  
Vol 316 (4) ◽  
pp. H900-H910 ◽  
Author(s):  
Akiyo Ito ◽  
Takashi Shiroto ◽  
Shigeo Godo ◽  
Hiroki Saito ◽  
Shuhei Tanaka ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cardiovascular Diseases ◽  
Reactive Nitrogen Species ◽  
Reactive Nitrogen ◽  
Oxygen Species ◽  
Nitrogen Species ◽  
Hindlimb Ischemia ◽  
Protein Modifications ◽  
Caveolin 1

Although increased levels of reactive oxygen species (ROS) are involved in the pathogenesis of cardiovascular diseases, the importance of physiological ROS has also been emerging. We have previously demonstrated that endothelium-derived H2O2 is an endothelium-dependent hyperpolarization (EDH) factor and that loss of endothelial caveolin-1 reduces EDH/H2O2 in the microcirculation. Caveolin-1 (Cav-1) is a scaffolding/regulatory protein that interacts with diverse signaling pathways, including angiogenesis. However, it remains unclear whether endothelial Cav-1 plays a role in ischemic angiogenesis by modulating EDH/H2O2. In the present study, we thus addressed this issue in a mouse model of hindlimb ischemia using male endothelium-specific Cav-1 (eCav-1) knockout (KO) mice. In isometric tension experiments with femoral arteries from eCav-1-KO mice, reduced EDH-mediated relaxations to acetylcholine and desensitization of sodium nitroprusside-mediated endothelium-independent relaxations were noted ( n = 4~6). An ex vivo aortic ring assay also showed that the extent of microvessel sprouting was significantly reduced in eCav-1-KO mice compared with wild-type (WT) littermates ( n = 12 each). Blood flow recovery at 4 wk assessed with a laser speckle flowmeter after femoral artery ligation was significantly impaired in eCav-1-KO mice compared with WT littermates ( n = 10 each) and was associated with reduced capillary density and muscle fibrosis in the legs ( n = 6 each). Importantly, posttranslational protein modifications by reactive nitrogen species and ROS, as evaluated by thiol glutathione adducts and nitrotyrosine, respectively, were both increased in eCav-1-KO mice ( n = 6~7 each). These results indicate that endothelial Cav-1 plays an important role in EDH-mediated vasodilatation and ischemic angiogenesis through posttranslational protein modifications by nitrooxidative stress in mice in vivo. NEW & NOTEWORTHY Although increased levels of reactive oxygen species (ROS) are involved in the pathogenesis of cardiovascular diseases, the importance of physiological ROS has also been emerging. The present study provides a line of novel evidence that endothelial caveolin-1 plays important roles in endothelium-dependent hyperpolarization and ischemic angiogenesis in hindlimb ischemia in mice through posttranslational protein modifications by reactive nitrogen species and ROS in mice in vivo.

scholarly journals Azanone (HNO): generation, stabilization and detection

2021 ◽  
Author(s):  
Cecilia Mariel Gallego ◽  
Agostina Mazzeo ◽  
Paola Vargas ◽  
Sebastián Suárez ◽  
Juan Pellegrino ◽  
...  
Keyword(s):  
Reactive Nitrogen Species ◽  
High Reactivity ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Biologically Relevant

HNO (nitroxyl, azanone), joined the ‘biologically relevant reactive nitrogen species’ family in the 2000s. Azanone is impossible to store due to its high reactivity and inherent low stability. Consequently, its...

Plasmonic Enhancement of Nitric Oxide Generation

Nanoscale ◽  
2021 ◽  
Author(s):  
Rachael Knoblauch ◽  
Chris Geddes
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Reactive Nitrogen Species ◽  
Reactive Oxygen ◽  
Reactive Nitrogen ◽  
Oxygen Species ◽  
Nitrogen Species ◽  
Plasmonic Enhancement ◽  
Cancer Treatments

While the utility of reactive oxygen species in photodynamic therapies for both cancer treatments and antimicrobial applications has received much attention, the inherent potential of reactive nitrogen species (RNS) including...

scholarly journals A Generator of Peroxynitrite Activatable with Red Light

2021 ◽  
Author(s):  
Cristina Parisi ◽  
Mariacristina Failla ◽  
Aurore Fraix ◽  
Luca Menilli ◽  
Francesca Moret ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Nitrogen Species ◽  
Red Light ◽  
Reactive Oxygen ◽  
Reactive Nitrogen ◽  
Oxygen Species ◽  
Nitrogen Species ◽  
New Horizons ◽  
Light Stimuli ◽  
Spatiotemporal Control

The generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) as “unconventional” therapeutics with precise spatiotemporal control by using light stimuli may open entirely new horizons for innovative...

scholarly journals What Did We Accomplish in Fighting Radical Species in Human Health?

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 466
Author(s):  
Rachid Skouta
Keyword(s):  
Reactive Oxygen Species ◽  
Human Health ◽  
Reactive Nitrogen Species ◽  
Reactive Oxygen ◽  
The Body ◽  
Reactive Nitrogen ◽  
Oxygen Species ◽  
Nitrogen Species ◽  
Radical Species ◽  
Physiological Level

Maintaining the physiological level of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in the body is highly important in the fight against radical species in the context of human health [...]

scholarly journals Uptake and diffusion of plasma-generated reactive nitrogen species through keratinized membrane

2019 ◽  
Vol 52 (19) ◽  
pp. 195201 ◽  
Author(s):  
Yeon-Ho Im ◽  
Zilan Xiong ◽  
Daniel T Elg ◽  
David B Graves
Keyword(s):  
Reactive Nitrogen Species ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
And Diffusion
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