scholarly journals Tumor progression locus 2/Cot is required for activation of extracellular regulated kinase in liver injury and toll-like receptor-induced TIMP-1 gene transcription in hepatic stellate cells in mice

Hepatology ◽  
2013 ◽  
Vol 57 (3) ◽  
pp. 1238-1249 ◽  
Author(s):  
Maria J. Perugorria ◽  
Lindsay B. Murphy ◽  
Nicola Fullard ◽  
Jayashree B. Chakraborty ◽  
Dimitra Vyrla ◽  
...  
Keyword(s):  
Liver Injury ◽  
Tumor Progression ◽  
Gene Transcription ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Toll Like Receptor ◽  
Extracellular Regulated Kinase

Selective activation of Toll-like receptor 7 in activated hepatic stellate cells may modulate their profibrogenic phenotype

2012 ◽  
Vol 447 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Ming-Huei Chou ◽  
Ying-Hsien Huang ◽  
Tsun-Mei Lin ◽  
Yung-Ying Du ◽  
Po-Chin Tsai ◽  
...  
Keyword(s):  
Liver Injury ◽  
Obstructive Jaundice ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Toll Like Receptor ◽  
Selective Activation ◽  
Down Regulation ◽  
Duct Ligation ◽  
Cholestatic Liver Injury ◽  
Tgf Β1

Cholestatic liver injury may activate HSCs (hepatic stellate cells) to a profibrogenic phenotype, contributing to liver fibrogenesis. We have previously demonstrated the involvement of TLR (Toll-like receptor) 7 in the pathogenesis of biliary atresia. In the present study we investigated the ability of TLR7 to modulate the profibrogenic phenotype in HSCs. Obstructive jaundice was associated with significant down-regulation of TLR7. Primary HSCs isolated from BDL (bile duct ligation) rats with obstructive jaundice exhibited reduced expression of TLR7 and increased expression of α-SMA (α-smooth muscle actin) and collagen-α1 compared with sham rats, reflecting HSC-mediated changes. Treatment of primary activated rat HSCs and rat T6 cells with CL075, a TLR7 and TLR8 ligand, significantly decreased expression of MCP-1 (monocyte chemotactic protein-1), TGF-β1 (transforming growth factor-β1), collagen-α1 and MMP-2 (matrix metalloproteinase-2), and inhibited cell proliferation and migration. In contrast, silencing TLR7 expression with shRNA (short hairpin RNA) in T6 cells effectively blocked the effects of CL075 stimulation, reversing the changes in MCP-1, TGF-β1 and collagen-α1 expression and accelerating cell migration. Our results indicate that obstructive jaundice is associated with down-regulation of TLR7 and up-regulation of profibrogenic gene expression in HSCs. Selective activation of TLR7 may modulate the profibrogenic phenotype in activated HSCs associated with cholestatic liver injury.

scholarly journals Differential regulation of TGF-β signal in hepatic stellate cells between acute and chronic rat liver injury

Hepatology ◽  
2002 ◽  
Vol 35 (1) ◽  
pp. 49-61 ◽  
Author(s):  
Yoshiya Tahashi ◽  
Koichi Matsuzaki ◽  
Masataka Date ◽  
Katsunori Yoshida ◽  
Fukiko Furukawa ◽  
...  
Keyword(s):  
Liver Injury ◽  
Rat Liver ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Differential Regulation

Mesalamine-induced B7-H1 expression on hepatic stellate cells attenuates autoimmune liver injury

2010 ◽  
Vol 41 (1) ◽  
pp. 79-86 ◽  
Author(s):  
Jeong-Hoon Lee ◽  
Jung-Hwan Yoon ◽  
Yu-Jeong Lee ◽  
Jong In Yang ◽  
Min-Sun Kwak ◽  
...  
Keyword(s):  
Liver Injury ◽  
Hepatic Stellate Cells ◽  
Stellate Cells
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