scholarly journals Plasma phospholipid transfer protein: A multifaceted protein with a key role in the assembly and secretion of apolipoprotein B-containing lipoproteins by the liver

Hepatology ◽  
2012 ◽  
Vol 56 (2) ◽  
pp. 415-418 ◽  
Author(s):  
Laurent Lagrost
Keyword(s):  
Apolipoprotein B ◽  
Protein A ◽  
Plasma Phospholipid ◽  
Phospholipid Transfer Protein ◽  
Transfer Protein ◽  
Phospholipid Transfer

scholarly journals Biosynthesis and secretion of human plasma phospholipid transfer protein

1998 ◽  
Vol 39 (10) ◽  
pp. 2021-2030 ◽  
Author(s):  
Jarkko Huuskonen ◽  
Matti Jauhiainen ◽  
Christian Ehnholm ◽  
Vesa M. Olkkonen
Keyword(s):  
Human Plasma ◽  
Plasma Phospholipid ◽  
Phospholipid Transfer Protein ◽  
Transfer Protein ◽  
Phospholipid Transfer

New therapeutic horizons for plasma phospholipid transfer protein (PLTP): Targeting endotoxemia, infection and sepsis

2021 ◽  
pp. 108105
Author(s):  
Thomas Gautier ◽  
Valérie Deckert ◽  
Maxime Nguyen ◽  
Catherine Desrumaux ◽  
David Masson ◽  
...  
Keyword(s):  
Plasma Phospholipid ◽  
Phospholipid Transfer Protein ◽  
Transfer Protein ◽  
Phospholipid Transfer

Plasma phospholipid transfer protein prevents vascular endothelium dysfunction by delivering α‐tocopherol to endothelial cells

1999 ◽  
Vol 13 (8) ◽  
pp. 883-892 ◽  
Author(s):  
Catherine Desrumaux ◽  
Valérie Deckert ◽  
Anne Athias ◽  
David Masson ◽  
Gérard Lizard ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelium ◽  
Plasma Phospholipid ◽  
Phospholipid Transfer Protein ◽  
Endothelium Dysfunction ◽  
Transfer Protein ◽  
Phospholipid Transfer

scholarly journals Measurement of Human Plasma Phospholipid Transfer Protein by Sandwich ELISA

2000 ◽  
Vol 46 (9) ◽  
pp. 1357-1364 ◽  
Author(s):  
Tomoichiro Oka ◽  
Takeshi Kujiraoka ◽  
Mayumi Ito ◽  
Makoto Nagano ◽  
Mitsuaki Ishihara ◽  
...  
Keyword(s):  
Chinese Hamster Ovary Cells ◽  
Sandwich Elisa ◽  
Chinese Hamster ◽  
Plasma Phospholipid ◽  
Hdl Cholesterol ◽  
Phospholipid Transfer Protein ◽  
Ovary Cells ◽  
Transfer Protein ◽  
Phospholipid Transfer ◽  
Hdl2 Cholesterol

Abstract Background: Plasma phospholipid transfer protein (PLTP) plays a central role in the remodeling of HDLs. Reliable and accurate methods for assaying PLTP concentration are required. Methods: A sandwich ELISA for PLTP has been developed, using two monoclonal antibodies against recombinant human PLTP (rhPLTP) expressed in Chinese hamster ovary cells. The ELISA allows for the quantification of PLTP in the range 0.625–15.0 ng/assay (1.2–30.0 mg/L). Intra- and interassay CVs were <3.0% and <4.2% respectively. The assay was used to quantify plasma PLTP concentrations in 132 Japanese subjects (75 males and 57 females). Results: PLTP concentrations were 12.0 ± 3.0 mg/L (mean ± SD; range, 4.9–20.5 mg/L). No sex difference was observed. Plasma PLTP concentration was positively correlated with HDL-cholesterol (r = 0.72; P <0.001), apolipoprotein (apo) A-I (r = 0.62; P <0.001) and HDL2-cholesterol (r = 0.72; P <0.001), and was negatively correlated with triacylglycerol (r = −0.45; P <0.001). There was no correlation with plasma apo A-II. These results agree with other evidence that plasma PLTP is associated with large apo A-I-containing lipoproteins. There was no correlation (r = −0.01) between plasma PLTP and plasma phosphatidylcholine transfer activity (range, 3.5–10.5 μmol · mL−1 · h−1), suggesting that PLTP may exist in active and inactive forms. Conclusion: This new ELISA will be of value for further studies of PLTP in health and disease.

scholarly journals Elevated Phospholipid Transfer Protein in Subjects with Multiple Sclerosis

2015 ◽  
Vol 2015 ◽  
pp. 1-5
Author(s):  
Roy A. Garvin
Keyword(s):  
Multiple Sclerosis ◽  
Structural Changes ◽  
Protein A ◽  
High Density Lipoproteins ◽  
Phospholipid Transfer Protein ◽  
Lipid Uptake ◽  
Myelin Lipid ◽  
Transfer Protein ◽  
Transfer Activity ◽  
Phospholipid Transfer

An anomaly in the plasma proteins of patients with multiple sclerosis detectable on SDS-PAGE has been reported. The molecular weight of the anomaly was the same as the phospholipid transfer protein. A metabolic protein was involved in lipid homeostasis and remodeling of the high density lipoproteins. We have identified the anomaly as the phospholipid transfer protein by western blot using antiphospholipid transfer antibodies. Activity assays showed that the phospholipid transfer activity was elevated in fasted plasma samples from subjects with MS compared to controls. Sequence analysis of the gene encoding the phospholipid transfer protein did not identify any mutations in the genetic structure, suggesting that the increase in activity was not due to structural changes in the protein, but may be due to one of the other proteins with which it forms active complexes. Altered phospholipid transfer activity is important because it could be implicated in the decreased lipid uptake and abnormal myelin lipids observed in multiple sclerosis. It has been shown that alteration in myelin lipid content is an epitope for autoimmunity. Therefore, lipid changes due to a defect in phospholipid transfer and/or uptake could potentially influence the course of the disease. Further research is needed to elucidate the role of the phospholipid transfer protein in subjects with multiple sclerosis.

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