scholarly journals Down-regulated microRNA-152 induces aberrant DNA methylation in hepatitis B virus-related hepatocellular carcinoma by targeting DNA methyltransferase 1

Hepatology ◽  
2010 ◽  
Vol 52 (1) ◽  
pp. 60-70 ◽  
Author(s):  
Jinfeng Huang ◽  
Yue Wang ◽  
Yingjun Guo ◽  
Shuhan Sun
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Methylation ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Methyltransferase ◽  
Dna Methyltransferase 1 ◽  
B Virus ◽  
Aberrant Dna Methylation ◽  
Methyltransferase 1

scholarly journals Hepatitis B virus X protein represses E-cadherin expression via activation of DNA methyltransferase 1

Oncogene ◽  
2005 ◽  
Vol 24 (44) ◽  
pp. 6617-6625 ◽  
Author(s):  
Jung-Ok Lee ◽  
Hyun Jin Kwun ◽  
Jin Kyu Jung ◽  
Kyung Hee Choi ◽  
Do Sik Min ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Dna Methyltransferase ◽  
Dna Methyltransferase 1 ◽  
X Protein ◽  
B Virus ◽  
E Cadherin ◽  
Methyltransferase 1

Aberrant DNA methylation profile of hepatitis B virus infection

2018 ◽  
Vol 91 (1) ◽  
pp. 81-92 ◽  
Author(s):  
Jinglan Jin ◽  
Hongqin Xu ◽  
Ruihong Wu ◽  
Junqi Niu ◽  
Shibo Li
Keyword(s):  
Dna Methylation ◽  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Methylation Profile ◽  
Hepatitis B Virus Infection ◽  
B Virus ◽  
Dna Methylation Profile ◽  
Aberrant Dna Methylation

scholarly journals Hepatitis B virus X protein suppresses caveolin-1 expression in hepatocellular carcinoma by regulating DNA methylation

BMC Cancer ◽  
2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Jun Yan ◽  
Qian Lu ◽  
Jiahong Dong ◽  
Xiaowu Li ◽  
Kuansheng Ma ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Methylation ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
X Protein ◽  
Caveolin 1 ◽  
B Virus

scholarly journals Differential DNA methylation associated with hepatitis B virus infection in hepatocellular carcinoma

2007 ◽  
Vol 121 (6) ◽  
pp. 1257-1264 ◽  
Author(s):  
Pei-Fen Su ◽  
Te-Chang Lee ◽  
Pei-Ju Lin ◽  
Po-Huang Lee ◽  
Yung-Ming Jeng ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Methylation ◽  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Hepatitis B Virus Infection ◽  
B Virus

scholarly journals DNA Methylation of Hepatitis B Virus (HBV) Genome Associated with the Development of Hepatocellular Carcinoma and Occult HBV Infection

2010 ◽  
Vol 202 (5) ◽  
pp. 700-704 ◽  
Author(s):  
Pushpinder Kaur ◽  
Anupam Paliwal ◽  
David Durantel ◽  
Pierre Hainaut ◽  
Jean‐Yves Scoazec ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Methylation ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hbv Infection ◽  
Occult Hbv Infection ◽  
Occult Hbv ◽  
B Virus

scholarly journals Hepatitis B Virus Demethylates PD-1 and Modulates the Changes of the Tumor Microenvironment in Hepatocellular Carcinoma Patients

2021 ◽  
Author(s):  
Weiguang Lian ◽  
Ruixue Lai ◽  
Jianhua Wu ◽  
Jingjing Zhang ◽  
Shengchao Li ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Methylation ◽  
T Cells ◽  
Hepatitis B Virus ◽  
Tumor Microenvironment ◽  
Hepatitis B ◽  
Hbv Infection ◽  
Chronic Hbv Infection ◽  
B Virus ◽  
Chronic Hbv

Abstract Background: Hepatitis B virus (HBV) constitutes a major global health burden. Previously study has found the expression of programmed cell death 1 (PD-1) is up-regulated during the chronic HBV infection. However, the mechanism of how HBV infection modulates the expression of PD-1 on CD8+T cells are not well understood. In the present study, we aimed to analyzed the role of DNA methylation in regulating the expression of PD-1 on CD8+T cells during HBV infection, we also aimed to evaluate the HBV induced changes of tumor microenvironment (TME).Methods: The methylation microarray was used to assess the profile changes of gene methylation upon chronic HBV infection. CD8+T cells were separated from peripheral blood of health volunteers and HCC patients including HBV related HCC (HBV-HCC) and non-viral HCC. The immune microenvironments of hepatocellular carcinoma (HCC) was interrogated by using Immunofluorescence staining. The PD-1 expression of CD8+T cells from peripheral blood were examined by western blot and flow cytometry. T cells function was determined by cytokine measurement. Sequenom MassARRAY platform was used to detected the DNA methylation status of PD-1 promoter.Results: HBV could drive 413 genes methylated while 3,023 genes including PD-1 demethylated, the reduced PD-1 expression and increased PD-1 demethylation was proved in HBV-HCC tissue. The subsequent analysis indicate that the expression of PD-1 differed by stages of HBV infection, it reduced on the cell membrane of HBV-transfected CD8+T cells upon transient HBV plasmid transfection which might mimic acute HBV infection, while upregulated on the HBV specific CD8+T cells by demethylation during chronic HBV infection. Interferon-gamma increased in the medium of HBV transfected CD8+T cells and interleukin-10 increased in the blood of chronic HBV infection patients. Increased tumor associated macrophage (TAM) cells and T regulatory cells (Tregs) invasion was identified in HBV-HCC tissue when compared with those from non-viral HCC tissue Conclusion: Our data suggested that the expression of PD-1 varies upon the stage of HBV infection, chronic HBV infection could drove the TME more immunosuppressive through PD-1 demethylation related CD8+T cells exhaustion as well as promotion of invaded Treg and TAM cells thereby to attenuating therapy of immune checkpoint inhibitors.

Sign in / Sign up

Export Citation Format

Share Document