scholarly journals Quantification of genotype 4 hepatitis C virus RNA by the COBAS AmpliPrep/COBAS TaqMan hepatitis C virus test

Hepatology ◽  
2009 ◽  
Vol 50 (5) ◽  
pp. 1679-1680 ◽  
Author(s):  
Jeffrey J. Germer ◽  
Carl E. Bommersbach ◽  
Dana M. Schmidt ◽  
Jordan L. Bendel ◽  
Joseph D. C. Yao
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatitis C Virus Rna ◽  
Genotype 4 ◽  
Cobas Taqman ◽  
Virus Rna

Differences between quantification of genotype 3 hepatitis C virus RNA by Versions 1.0 and 2.0 of the COBAS AmpliPrep/COBAS TaqMan HCV Test

2017 ◽  
Vol 55 (7) ◽  
Author(s):  
Virginia M. Pierce ◽  
Jacqueline S. Eversley ◽  
Thuy K. Tran ◽  
Eric S. Rosenberg
Keyword(s):  
Hepatitis C Virus ◽  
Viral Load ◽  
Hepatitis C ◽  
Genotype 3 ◽  
Hepatitis C Virus Rna ◽  
Hcv Genotype ◽  
Cobas Taqman ◽  
Viral Loads ◽  
Rna Quantification ◽  
Virus Rna

AbstractBackground:Differences between the designs of hepatitis C virus (HCV) viral load assays can result in genotype-related variability in RNA quantification. We tested paired aliquots of plasma specimens from HCV-infected individuals using two versions (v1.0 and v2.0) of the Roche COBAS AmpliPrep/COBAS TaqMan HCV Test (CAP/CTM HCV) and noted variability between results for a subset of specimens; we then sought to determine whether discrepant results were more prevalent among specific HCV genotypes.Methods:Archived and prospectively-collected plasma samples from 114 unique patients were tested using CAP/CTM HCV v1.0 and v2.0. The HCV genotype result for each patient was determined by retrospectively reviewing laboratory records.Results:All (46/46) specimens with quantifiable viral loads from patients with genotype 1 or 2 infection had CAP/CTM HCV v1.0 and v2.0 results that were within 0.5 logConclusions:In patients infected with HCV genotype 3, sequential CAP/CTM HCV viral load results should be compared with caution and interpreted in the context of the specific assay version used.

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