scholarly journals Ammonia induces RNA oxidation in cultured astrocytes and brainin vivo

Hepatology ◽  
2008 ◽  
Vol 48 (2) ◽  
pp. 567-579 ◽  
Author(s):  
Boris Görg ◽  
Natalia Qvartskhava ◽  
Verena Keitel ◽  
Hans J. Bidmon ◽  
Oliver Selbach ◽  
...  
Keyword(s):  
Rna Oxidation ◽  
Cultured Astrocytes

Ammonia increases RNA oxidation in cultured astrocytes and brain in vivo

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
B Görg ◽  
N Qvartskhava ◽  
V Keitel ◽  
HJ Bidmon ◽  
F Schliess ◽  
...  
Keyword(s):  
Rna Oxidation ◽  
Cultured Astrocytes

Preparation of cultured astrocytes for x-ray microanalysis

1989 ◽  
Vol 47 ◽  
pp. 988-989
Author(s):  
N.K.R. Smith ◽  
K.E. Hunter ◽  
P. Mobley ◽  
L.P. Felpel
Keyword(s):  
Cultured Cells ◽  
Elemental Content ◽  
Elemental Distribution ◽  
Sprague Dawley ◽  
X Ray ◽  
Cultured Astrocytes ◽  
Freeze Dried ◽  
Ultimate Objective

Electron probe energy dispersive x-ray microanalysis (XRMA) offers a powerful tool for the determination of intracellular elemental content of biological tissue. However, preparation of the tissue specimen , particularly excitable central nervous system (CNS) tissue , for XRMA is rather difficult, as dissection of a sample from the intact organism frequently results in artefacts in elemental distribution. To circumvent the problems inherent in the in vivo preparation, we turned to an in vitro preparation of astrocytes grown in tissue culture. However, preparations of in vitro samples offer a new and unique set of problems. Generally, cultured cells, growing in monolayer, must be harvested by either mechanical or enzymatic procedures, resulting in variable degrees of damage to the cells and compromised intracel1ular elemental distribution. The ultimate objective is to process and analyze unperturbed cells. With the objective of sparing others from some of the same efforts, we are reporting the considerable difficulties we have encountered in attempting to prepare astrocytes for XRMA.Tissue cultures of astrocytes from newborn C57 mice or Sprague Dawley rats were prepared and cultured by standard techniques, usually in T25 flasks, except as noted differently on Cytodex beads or on gelatin. After different preparative procedures, all samples were frozen on brass pins in liquid propane, stored in liquid nitrogen, cryosectioned (0.1 μm), freeze dried, and microanalyzed as previously reported.

Two Metabolic Fuels, Glucose and Lactate, Differentially Modulate Exocytotic Glutamate Release from Cultured Astrocytes

2021 ◽  
Author(s):  
Vedrana Montana ◽  
Daniel Flint ◽  
Helle S. Waagepetersen ◽  
Arne Schousboe ◽  
Vladimir Parpura
Keyword(s):  
Glutamate Release ◽  
Cultured Astrocytes

scholarly journals Thyroxine-dependent modulation of actin polymerization in cultured astrocytes. A novel, extranuclear action of thyroid hormone.

1990 ◽  
Vol 265 (9) ◽  
pp. 5296-5302
Author(s):  
C A Siegrist-Kaiser ◽  
C Juge-Aubry ◽  
M P Tranter ◽  
D M Ekenbarger ◽  
J L Leonard
Keyword(s):  
Thyroid Hormone ◽  
Actin Polymerization ◽  
Cultured Astrocytes

Ryanodine-induced intracellular calcium mobilisation in cultured astrocytes

Glia ◽  
1994 ◽  
Vol 12 (2) ◽  
pp. 128-134 ◽  
Author(s):  
David Langley ◽  
Brian Pearce
Keyword(s):  
Intracellular Calcium ◽  
Cultured Astrocytes

Mechanistic studies of DNA and RNA oxidation by macrocyclic nickel complexes.

1993 ◽  
Vol 51 (1-2) ◽  
pp. 517
Author(s):  
James G. Muller ◽  
Ping Zheng ◽  
Chien-Chung Cheng ◽  
Ren-Hwa Yeh ◽  
Steven E. Rokita ◽  
...  
Keyword(s):  
Nickel Complexes ◽  
Mechanistic Studies ◽  
Dna And Rna ◽  
Rna Oxidation ◽  
Macrocyclic Nickel
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