Isolation and functional identification of a novel human hepatic growth factor: Hepatopoietin Cn

Hepatology ◽  
2008 ◽  
Vol 47 (3) ◽  
pp. 986-995 ◽  
Author(s):  
Chun-Ping Cui ◽  
Da-Jin Zhang ◽  
Bing-Xing Shi ◽  
Shao-Jun Du ◽  
Dan-Li Wu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Functional Identification ◽  
Hepatic Growth Factor

scholarly journals Investigating the Relationship between Serum Level of s-Met (Soluble Hepatic Growth Factor Receptor) and Preeclampsia in the First and Second Trimesters of Pregnancy

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Farshad Naghshvar ◽  
Zhila Torabizadeh ◽  
Narges Moslemi Zadeh ◽  
Hooman Mirbaha ◽  
Parand Gheshlaghi
Keyword(s):  
Growth Factor ◽  
Serum Level ◽  
Growth Factor Receptor ◽  
Serum Levels ◽  
Severe Preeclampsia ◽  
Soluble Form ◽  
Control Group ◽  
Case Group ◽  
Mortality And Morbidity ◽  
Hepatic Growth Factor

Introduction. Preeclampsia (PE) is an important cause of mortality and morbidity for mothers, fetuses, and the newborns. Placenta plays a pivotal role in pathogenesis of PE. Hepatic growth factor (HGF) is a cytokine expressed by the mesenchymal stalk of placental villi during pregnancy and assumes a paracrine role in trophoblasts which express its receptor (c-MET). In the present study, we investigate the diagnostic value of s-Met (the soluble form of the receptor) in the first and second trimesters of pregnancy for early diagnosis of preeclampsia. Method and Materials. This is a case-control study conducted on 95 pregnant women. The serum level of s-Met was measured in the first and second trimesters, and the participants were followed until delivery. 44 individuals with preeclampsia (the case group) and 51 individuals without preeclampsia (the control group) were evaluated. Results. Serum level of s-Met in preeclamptic participants was lower than that of the control group in both the first and the second trimesters (P<0.0001). In addition, serum levels of s-Met were significantly lower during the first and second trimesters in patients with early, severe preeclampsia compared to those with late, mild preeclampsia (P<0.0001). The sensitivity and specificity of s-Met in the first and second trimesters were, respectively, (83%, 94%) and (77%, 94%) for early preeclampsia and (88%, 92%) and (86%, 98%) for severe preeclampsia. Conclusion. Considering our findings, serum level of s-Met may be used as a predictive factor for early detection of preeclampsia. Further research is required to corroborate the functional and therapeutic value of s-Met in preeclampsia.

scholarly journals Gene similarity between hepatitis C virus and human proteins: A blood transfusion problem

2005 ◽  
Vol 58 (11-12) ◽  
pp. 582-586 ◽  
Author(s):  
Nada Vasiljevic ◽  
Ljiljana Markovic
Keyword(s):  
Hepatitis C ◽  
Growth Factor ◽  
Tyrosine Kinase ◽  
Nitrogen Oxide ◽  
Molecular Mimicry ◽  
Highly Sensitive ◽  
Nitrogen Oxide Synthase ◽  
Human Proteins ◽  
Hepatic Growth Factor ◽  
Oxide Synthase

Introduction Hepatitis C is a post-transfusion hepatitis which causes serious problems in blood transfusion. Blood testing requires highly sensitive and specific assays with high predictive value. Genomic characteristics of hepatitis C virus According to recommendations of International Association for the study of Liver Diseases etiological diagnosis of hepatitis is based on highly sensitive third generation assays: epitopes in the NS5 region comprising noncoding sequence UTR with 324-341 well conserved pair of homologous basis in 92% HCV genomes, therefore appropriate for virus RNA detection. Development of assays for hepatitis virus The first generation of immunoenzyme tests (IET) were based on detection of antibodies on antigen c 100-3, which is a part of the NS4 region of HCV genome. The second generation of tests with two recombinant proteins - c22-3 and c200, achieved higher sensitivity of assays. The third generation included epitopes from NS5 region, and removed the antigen c100-3. Development of autoimmunity Autoimmunity is a pathophysiological mechanism that's leads to chronic inflammatory diseases. Autoimunity is characterized by loss of tolerance towards self-antigens. Viral hepatitis C is associated with development of autoimmune phenomena. Molecular mimicry Molecular mimicry, as a mechanism of autoimmunity, was investigated to establish cross-Reactive immune reactions between HCV antigen and human nitrogen-oxide synthase, Tyrosine kinase Lck, and hepatic growth factor activator. Cross reactivity between HCV proteins and human proteins HCV capsid proteins initiate the autoimmune process in the liver because of cross reaction of antibodies with human Gor protein 19-27, which causes autoimmune chronic hepatitis. However, analysis of human protein from protein basis Swissprot shows homology between NS5 region and 3 human protein nitrogen oxide synthases, tyrosine kinase-Lck, proto-oncogene and hepatic growth factor activator. According to protein data analysis and competitive in vitro experiments, it was concluded that presence of auto-antibodies is probably the consequence of cross reactive immune response. Conclusion Homology of amino acid sequences in the NS5 region of the HCV genome with nitrogen-oxide synthase, tyrosine kinase-Lck, and hepatic growth factor activator, causes auto-immune phenomena in HC, and can be a model for researching autoimmunity and human virus-induced autoimmune diseases.

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