scholarly journals Characterization of the hepatitis C virus E2 epitope defined by the broadly neutralizing monoclonal antibody AP33

Hepatology ◽  
2006 ◽  
Vol 43 (3) ◽  
pp. 592-601 ◽  
Author(s):  
Alexander W. Tarr ◽  
Ania M. Owsianka ◽  
Judith M. Timms ◽  
C. Patrick McClure ◽  
Richard J. P. Brown ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Neutralizing Monoclonal Antibody

scholarly journals Identification and Characterization of Broadly Neutralizing Human Monoclonal Antibodies Directed against the E2 Envelope Glycoprotein of Hepatitis C Virus

2009 ◽  
Vol 83 (23) ◽  
pp. 12473-12482 ◽  
Author(s):  
Teresa J. Broering ◽  
Kerry A. Garrity ◽  
Naomi K. Boatright ◽  
Susan E. Sloan ◽  
Frantisek Sandor ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Glycoprotein ◽  
Cross Reactivity ◽  
Human Antibody ◽  
Human Monoclonal Antibodies ◽  
Genotype 1A ◽  
Neutralizing Monoclonal Antibody

ABSTRACT Nearly all livers transplanted into hepatitis C virus (HCV)-positive patients become infected with HCV, and 10 to 25% of reinfected livers develop cirrhosis within 5 years. Neutralizing monoclonal antibody could be an effective therapy for the prevention of infection in a transplant setting. To pursue this treatment modality, we developed human monoclonal antibodies (HuMAbs) directed against the HCV E2 envelope glycoprotein and assessed the capacity of these HuMAbs to neutralize a broad panel of HCV genotypes. HuMAb antibodies were generated by immunizing transgenic mice containing human antibody genes (HuMAb mice; Medarex Inc.) with soluble E2 envelope glycoprotein derived from a genotype 1a virus (H77). Two HuMAbs, HCV1 and 95-2, were selected for further study based on initial cross-reactivity with soluble E2 glycoproteins derived from genotypes 1a and 1b, as well as neutralization of lentivirus pseudotyped with HCV 1a and 1b envelope glycoproteins. Additionally, HuMAbs HCV1 and 95-2 potently neutralized pseudoviruses from all genotypes tested (1a, 1b, 2b, 3a, and 4a). Epitope mapping with mammalian and bacterially expressed proteins, as well as synthetic peptides, revealed that HuMAbs HCV1 and 95-2 recognize a highly conserved linear epitope spanning amino acids 412 to 423 of the E2 glycoprotein. The capacity to recognize and neutralize a broad range of genotypes, the highly conserved E2 epitope, and the fully human nature of the antibodies make HuMAbs HCV1 and 95-2 excellent candidates for treatment of HCV-positive individuals undergoing liver transplantation.

scholarly journals Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1

Virology ◽  
2018 ◽  
Vol 514 ◽  
pp. 30-41 ◽  
Author(s):  
Ahmed Atef Mesalam ◽  
Isabelle Desombere ◽  
Ali Farhoudi ◽  
Freya Van Houtte ◽  
Lieven Verhoye ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Envelope Glycoprotein ◽  
Human Monoclonal Antibody ◽  
Terminal Region ◽  
Virus Envelope ◽  
Antibody Targeting

Production and Characterization of Monoclonal Antibody Specific for NS3 Helicase of Hepatitis C Virus

Hybridoma ◽  
2008 ◽  
Vol 27 (3) ◽  
pp. 181-186 ◽  
Author(s):  
Jing Yang ◽  
Ying-Feng Lei ◽  
Wen Yin ◽  
San-Hua Wei ◽  
Qun-Xing An ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Hepatitis C Virus ◽  
Hepatitis C

scholarly journals Characterization of a hepatitis C virus genotype 1 divergent isolate from an HIV-1 coinfected individual in Germany assigned to a new subtype 1o

2019 ◽  
Vol 16 (1) ◽  
Author(s):  
Bo Wang ◽  
Luise Krüger ◽  
Patrycja Machnowska ◽  
Amare Eshetu ◽  
Barbara Gunsenheimer-Bartmeyer ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Genotype 1 ◽  
Virus Genotype ◽  
Hiv 1

scholarly journals Characterization of Resistance to Non-obligate Chain-terminating Ribonucleoside Analogs That Inhibit Hepatitis C Virus Replicationin Vitro

2003 ◽  
Vol 278 (49) ◽  
pp. 49164-49170 ◽  
Author(s):  
Giovanni Migliaccio ◽  
Joanne E. Tomassini ◽  
Steven S. Carroll ◽  
Licia Tomei ◽  
Sergio Altamura ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C
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