VPAC1 expression is regulated by FXR agonists in the human gallbladder epithelium

Hepatology ◽  
2005 ◽  
Vol 42 (3) ◽  
pp. 549-557 ◽  
Author(s):  
Nicolas Chignard ◽  
Martine Mergey ◽  
Véronique Barbu ◽  
Laetitia Finzi ◽  
Emmanuel Tiret ◽  
...  
Keyword(s):  
Gallbladder Epithelium ◽  
Human Gallbladder ◽  
Human Gallbladder Epithelium

Bile salts potentiate adenylyl cyclase activity and cAMP-regulated secretion in human gallbladder epithelium

2003 ◽  
Vol 284 (2) ◽  
pp. G205-G212 ◽  
Author(s):  
Nicolas Chignard ◽  
Martine Mergey ◽  
Danielle Veissière ◽  
Raoul Poupon ◽  
Jacqueline Capeau ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Adenylyl Cyclase ◽  
Bile Salts ◽  
Primary Cultures ◽  
Cyclase Activity ◽  
Gallbladder Epithelium ◽  
Camp Production ◽  
Adenylyl Cyclase Activity ◽  
Human Gallbladder ◽  
Human Gallbladder Epithelium

Fluid and ion secretion in the gallbladder is mainly triggered by the intracellular second messenger cAMP. We examined the action of bile salts on the cAMP-dependent pathway in the gallbladder epithelium. Primary cultures of human gallbladder epithelial cells were exposed to agonists of the cAMP pathway and/or to bile salts. Taurochenodeoxycholate and tauroursodeoxycholate increased forskolin-induced cAMP accumulation to a similar extent, without affecting cAMP basal levels. This potentiating effect was abrogated after PKC inhibition, whereas both taurochenodeoxycholate and tauroursodeoxycholate induced PKC-α and -δ translocation to cell membranes. Consistent with a PKC-mediated stimulation of cAMP production, the expression of six adenylyl cyclase isoforms, including PKC-regulated isoforms 5 and 7, was identified in human gallbladder epithelial cells. cAMP-dependent chloride secretion induced by isoproterenol, a β-adrenergic agonist, was significantly increased by taurochenodeoxycholate and by tauroursodeoxycholate. In conclusion, endogenous and therapeutic bile salts via PKC regulation of adenylyl cyclase activity potentiate cAMP production in the human gallbladder epithelium. Through this action, bile salts may increase fluid secretion in the gallbladder after feeding.

Bacterial adherence to human gallbladder epithelium

Surgery Today ◽  
1992 ◽  
Vol 22 (6) ◽  
pp. 504-507 ◽  
Author(s):  
Satoshi Sakurai ◽  
Nagao Shinagawa ◽  
Takuji Fukui ◽  
Jiro Yura
Keyword(s):  
Bacterial Adherence ◽  
Gallbladder Epithelium ◽  
Human Gallbladder ◽  
Human Gallbladder Epithelium

scholarly journals Subcellular localization of the ABCG2 transporter in normal and malignant human gallbladder epithelium

2004 ◽  
Vol 84 (8) ◽  
pp. 1024-1036 ◽  
Author(s):  
Sylvia Aust ◽  
Peter Obrist ◽  
Walter Jaeger ◽  
Martin Klimpfinger ◽  
Gerhard Tucek ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Gallbladder Epithelium ◽  
Human Gallbladder ◽  
Human Gallbladder Epithelium

Primary culture of human gallbladder epithelium: A new tool for studying gallstone formation

1998 ◽  
Vol 114 ◽  
pp. A533
Author(s):  
KE Morgenstern ◽  
GB Balsara ◽  
ZR Abedin ◽  
JJ Roslyn ◽  
MZ Abedin ◽  
...  
Keyword(s):  
Primary Culture ◽  
Gallstone Formation ◽  
Gallbladder Epithelium ◽  
Human Gallbladder ◽  
Human Gallbladder Epithelium

Organotypic Culture of Human Gallbladder Epithelium

1995 ◽  
Vol 63 (1) ◽  
pp. 16-22 ◽  
Author(s):  
Dolphine P. Oda ◽  
Lydia Eng ◽  
Christopher E. Savard ◽  
Mike Newcomer ◽  
W.Geoffrey Haigh ◽  
...  
Keyword(s):  
Organotypic Culture ◽  
Gallbladder Epithelium ◽  
Human Gallbladder ◽  
Human Gallbladder Epithelium

S1248 Expression of Peroxisome Proliferator-Activator Receptor Gamma in Human Gallbladder Epithelium and Gallstones

2010 ◽  
Vol 138 (5) ◽  
pp. S-213
Author(s):  
Hyoung-Chul Oh ◽  
Jae Hyuk Do ◽  
Beom Jin Kim ◽  
Jeong Wook Kim ◽  
Jae G. Kim ◽  
...  
Keyword(s):  
Peroxisome Proliferator ◽  
Gallbladder Epithelium ◽  
Human Gallbladder ◽  
Human Gallbladder Epithelium
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